Detection of feline immunodeficiency virus by neutral red-based loop-mediated isothermal amplification assay.
feline immunodeficiency virus
loop-mediated isothermal amplification
molecular diagnosis
neutral red
Journal
Veterinary world
ISSN: 0972-8988
Titre abrégé: Vet World
Pays: India
ID NLM: 101504872
Informations de publication
Date de publication:
Jan 2024
Jan 2024
Historique:
received:
20
09
2023
accepted:
13
12
2023
medline:
26
2
2024
pubmed:
26
2
2024
entrez:
26
2
2024
Statut:
ppublish
Résumé
Feline immunodeficiency virus (FIV) is a retroviral pathogen globally responsible for immunodeficiency disease in cats. However, the current diagnosis based on antibody detection has limitations and can also produce false-positive results. This study aimed to develop a one-pot loop-mediated isothermal amplification (LAMP) process integrated with neutral red (NR-LAMP) assay for detection of FIV proviral DNA. We developed a one-pot, The developed NR-LAMP was capable of amplifying at an optimum temperature of 65°C for 40 min. No cross-amplification was detected between FIV and other feline viruses tested, indicating the high specificity (98.44%) of the novel FIV-LAMP primer. Our NR-LAMP assay has a detection limit of 4.2 × 10 These results support the suitability of NR-LAMP as a potential future alternative clinical molecular approach for further use in the diagnosis of FIV-infected cats.
Sections du résumé
Background and Aim
UNASSIGNED
Feline immunodeficiency virus (FIV) is a retroviral pathogen globally responsible for immunodeficiency disease in cats. However, the current diagnosis based on antibody detection has limitations and can also produce false-positive results. This study aimed to develop a one-pot loop-mediated isothermal amplification (LAMP) process integrated with neutral red (NR-LAMP) assay for detection of FIV proviral DNA.
Materials and Methods
UNASSIGNED
We developed a one-pot,
Results
UNASSIGNED
The developed NR-LAMP was capable of amplifying at an optimum temperature of 65°C for 40 min. No cross-amplification was detected between FIV and other feline viruses tested, indicating the high specificity (98.44%) of the novel FIV-LAMP primer. Our NR-LAMP assay has a detection limit of 4.2 × 10
Conclusion
UNASSIGNED
These results support the suitability of NR-LAMP as a potential future alternative clinical molecular approach for further use in the diagnosis of FIV-infected cats.
Identifiants
pubmed: 38406374
doi: 10.14202/vetworld.2024.72-81
pii: Vetworld-17-72
pmc: PMC10884571
doi:
Types de publication
Journal Article
Langues
eng
Pagination
72-81Informations de copyright
Copyright: © Saejung, et al.
Déclaration de conflit d'intérêts
The authors declare that they have no competing interests.