Variants of a major DNA satellite discriminate parental subgenomes in a hybrid parthenogenetic lizard Darevskia unisexualis (Darevsky, 1966).
centromere
chromosome
fluorescence in situ hybridization
hybrid
parthenogenesis
satellite DNA
tandem repeats
Journal
Journal of experimental zoology. Part B, Molecular and developmental evolution
ISSN: 1552-5015
Titre abrégé: J Exp Zool B Mol Dev Evol
Pays: United States
ID NLM: 101168228
Informations de publication
Date de publication:
26 Feb 2024
26 Feb 2024
Historique:
revised:
12
12
2023
received:
31
08
2023
accepted:
30
01
2024
medline:
26
2
2024
pubmed:
26
2
2024
entrez:
26
2
2024
Statut:
aheadofprint
Résumé
Hybrid parthenogenetic animals are an exceptionally interesting model for studying the mechanisms and evolution of sexual and asexual reproduction. A diploid parthenogenetic lizard Darevskia unisexualis is a result of an ancestral cross between a maternal species Darevskia raddei nairensis and a paternal species Darevskia valentini and presents a unique opportunity for a cytogenetic and computational analysis of a hybrid karyotype. Our previous results demonstrated a significant divergence between the pericentromeric DNA sequences of the parental Darevskia species; however, an in-depth comparative study of their pericentromeres is still lacking. Here, using target sequencing of microdissected pericentromeric regions, we reveal and compare the repertoires of the pericentromeric tandem repeats of the parental Darevskia lizards. We found species-specific sequences of the major pericentromeric tandem repeat CLsat, which allowed computational prediction and experimental validation of fluorescent DNA probes discriminating parental chromosomes within the hybrid karyotype of D. unisexualis. Moreover, we have implemented a generalizable computational method, based on the optimization of the Levenshtein distance between tandem repeat monomers, for finding species-specific fluorescent probes for pericentromere staining. In total, we anticipate that our comparative analysis of Darevskia pericentromeric repeats, the species-specific fluorescent probes that we found and the pipeline that we developed will form a basis for the future detailed cytogenomic studies of a wide range of natural and laboratory hybrids.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : State contract of VIGG RAS (cytogenetic experiments)
ID : 0092-2022-0002
Organisme : Science Committee of Republic of Armenia (microscopy)
ID : 23IRF-1F06
Organisme : Russian National Foundation (expeditions and keeping the animals)
ID : 22-14-00227
Informations de copyright
© 2024 Wiley Periodicals LLC.
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