Characterization and immunological effect of outer membrane vesicles from Pasteurella multocida on macrophages.
Inflammatory response
Outer membrane vesicles
Pasteurella multocida
Proteomics
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
26 Feb 2024
26 Feb 2024
Historique:
received:
13
11
2023
accepted:
06
02
2024
revised:
11
01
2024
medline:
26
2
2024
pubmed:
26
2
2024
entrez:
26
2
2024
Statut:
epublish
Résumé
Pasteurella multocida is an important bacterial pathogen that can cause diseases in both animals and humans. Its elevated morbidity and mortality rates in animals result in substantial economic repercussions within the livestock industry. The prevention of diseases caused by P. multocida through immunization is impeded by the absence of a safe and effective vaccine. Outer membrane vesicles (OMVs) secreted from the outer membrane of Gram-negative bacteria are spherical vesicular structures that encompass an array of periplasmic components in conjunction with a diverse assortment of lipids and proteins. These vesicles can induce antibacterial immune responses within the host. P. multocida has been shown to produce OMVs. Nonetheless, the precise characteristics and immunomodulatory functions of P. multocida OMVs have not been fully elucidated. In this study, OMVs were isolated from P. multocida using an ultrafiltration concentration technique, and their morphology, protein constitution, and immunomodulatory properties in RAW264.7 cells were studied. Transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA) revealed that the OMVs exhibited typical spherical and bilayered lipid vesicular architecture, exhibiting an average diameter of approximately 147.5 nm. The yield of OMVs was 2.6 × 10
Identifiants
pubmed: 38407600
doi: 10.1007/s00253-024-13060-2
pii: 10.1007/s00253-024-13060-2
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
238Subventions
Organisme : China Agriculture Research System of MOF and MARA
ID : Grant No. CARS-43-C-2
Organisme : National Natural Science Foundation of China
ID : Grant No. 32002323
Organisme : Key Research and Development Program of Zhejiang Province
ID : No. 2021C02007 and No. 2019C02052
Organisme : Pioneer and Leading Goose R&D Program of Zhejiang
ID : Grant No. 2023C02047
Informations de copyright
© 2024. The Author(s).
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