Probing the legitimate initiation of RNA synthesis by Qβ replicase with oligonucleotide primers.
5′-triphosphate
RNA secondary structure
RNA-directed RNA polymerase
elongation factor EF-Tu
single-stranded RNA phage
template recognition
Journal
FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157
Informations de publication
Date de publication:
26 Feb 2024
26 Feb 2024
Historique:
revised:
24
01
2024
received:
14
11
2023
accepted:
31
01
2024
medline:
27
2
2024
pubmed:
27
2
2024
entrez:
26
2
2024
Statut:
aheadofprint
Résumé
Oligoribonucleotides complementary to the template 3' terminus were tested for their ability to initiate RNA synthesis on legitimate templates capable of exponential amplification by Qβ replicase. Oligonucleotides shorter than the distance to the nearest predicted template hairpin proved able to serve as primers, with the optimal length varying for different templates, suggesting that during initiation the template retains its native fold incorporating the 3' terminus. The priming activity of an oligonucleotide is greatly enhanced by its 5'-triphosphate group, the effect being strongly dependent on Mg
Identifiants
pubmed: 38408766
doi: 10.1002/1873-3468.14833
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Russian Foundation for Basic Research
ID : 20-04-00194
Informations de copyright
© 2024 Federation of European Biochemical Societies.
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