BmPGPR-L4 is a negative regulator of the humoral immune response in the silkworm Bombyx mori.
BmPGPR-L4
Bombyx mori
antimicrobial peptides
immune response
peptidoglycan recognition proteins
Journal
Archives of insect biochemistry and physiology
ISSN: 1520-6327
Titre abrégé: Arch Insect Biochem Physiol
Pays: United States
ID NLM: 8501752
Informations de publication
Date de publication:
Feb 2024
Feb 2024
Historique:
revised:
07
01
2024
received:
16
11
2023
accepted:
11
02
2024
medline:
27
2
2024
pubmed:
27
2
2024
entrez:
27
2
2024
Statut:
ppublish
Résumé
Toll, immune deficiency and prophenoloxidase cascade represent vital immune signaling pathways in insects. Peptidoglycan recognition proteins (PGRPs) are innate immune receptors that activate and regulate the immune signaling pathways. Previously, we reported that BmPGPR-L4 was induced in the silkworm Bombyx mori larvae by bacteria and peptidoglycan challenges. Here, we focused on the function of BmPGRP-L4 in regulating the expression of antimicrobial peptides (AMPs). The hemolymph from BmPGRP-L4-silenced larvae exhibited an enhanced inhibitory effect on the growth of Escherichia coli, either by growth curve or inhibitory zone experiments. Coincidentally, most of the AMP genes were upregulated after RNAi of BmPGRP-L4. Oral administration of heat-inactivated E. coli and Staphylococcus aureus after RNAi of BmPGRP-L4 resulted in the increased expression of BmPGRP-L4 in different tissues of the silkworm larvae, revealing an auto-regulatory mechanism. By contrast, the expression of most AMP genes was downregulated by oral bacterial administration after RNAi of BmPGRP-L4. The above results demonstrate that BmPGRP-L4 recognizes bacterial pathogen-associated molecular patterns and negatively regulates AMP expression to achieve immunological homeostasis. As a negative regulator, BmPGPR-L4 is proposed to be involved in the feedback regulation of the immune signaling pathways of the silkworm to prevent excessive activation of the immune response.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e22093Subventions
Organisme : Science and Technology Program of Guangzhou
ID : 202102010465
Organisme : National Natural Science Foundation of China
ID : 31501898
Organisme : Guangzhou University Graduate Innovation Ability Cultivation Funding Program
ID : 2022GDJC-M17
Informations de copyright
© 2024 Wiley Periodicals LLC.
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