Synchrotron-based propagation-based imaging (PBI) is ideally suited for lung imaging and has successfully been applied in a variety of Here, we demonstrate our findings of using PBI-based planar and CT imaging with two different detectors-MÖNCH 0.3 direct conversion detector and a complementary metal-oxide-semiconductor (CMOS) detector (Photonics Science)-in an Ovalbumin induced experimental allergic airway disease mouse model in comparison with healthy controls. The mice were imaged free breathing under isoflurane anesthesia. At x-ray dose levels below those once used by commercial small animal CT devices at similar spatial resolutions, we were able to resolve structural changes at a pixel size down to 25 μm and demonstrate the reduction in elastic recoil in the asthmatic mice in cinematic planar x-ray imaging with a frame rate of up to 100 fps. Thus, we believe that our approach will permit longitudinal small animal lung disease studies, closely following the mice over longer time spans.

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Copyright © 2024 Dullin, Albers, Tagat, Lorenzon, D'Amico, Chiriotti, Sodini, Dreossi, Alves, Bergamaschi and Tromba.

Déclaration de conflit d'intérêts

SC and AL are now working for companies but worked for public research organizations during duration of the experiment. AL was employed by Innova S.p.A. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.