Chemoenzymatic indican for light-driven denim dyeing.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
27 Feb 2024
27 Feb 2024
Historique:
received:
01
08
2023
accepted:
01
02
2024
medline:
28
2
2024
pubmed:
28
2
2024
entrez:
27
2
2024
Statut:
epublish
Résumé
Blue denim, a billion-dollar industry, is currently dyed with indigo in an unsustainable process requiring harsh reducing and alkaline chemicals. Forming indigo directly in the yarn through indican (indoxyl-β-glucoside) is a promising alternative route with mild conditions. Indican eliminates the requirement for reducing agent while still ending as indigo, the only known molecule yielding the unique hue of blue denim. However, a bulk source of indican is missing. Here, we employ enzyme and process engineering guided by techno-economic analyses to develop an economically viable drop-in indican synthesis technology. Rational engineering of PtUGT1, a glycosyltransferase from the indigo plant, alleviated the severe substrate inactivation observed with the wildtype enzyme at the titers needed for bulk production. We further describe a mild, light-driven dyeing process. Finally, we conduct techno-economic, social sustainability, and comparative life-cycle assessments. These indicate that the presented technologies have the potential to significantly reduce environmental impacts from blue denim dyeing with only a modest cost increase.
Identifiants
pubmed: 38413572
doi: 10.1038/s41467-024-45749-3
pii: 10.1038/s41467-024-45749-3
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1489Subventions
Organisme : Novo Nordisk Fonden (Novo Nordisk Foundation)
ID : NNF10CC1016517
Organisme : Novo Nordisk Fonden (Novo Nordisk Foundation)
ID : NNF20CC0035580
Organisme : Novo Nordisk Fonden (Novo Nordisk Foundation)
ID : NNF16OC0019088
Organisme : Carlsbergfondet (Carlsberg Foundation)
ID : CF18-0631
Informations de copyright
© 2024. The Author(s).
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