Rapid quantitative detection system for measles virus-neutralizing antibodies using HiBiT-tagged virus-like particles.
HiBiT
LgBiT
fusion
hemagglutinin
measles virus
virus neutralization test
virus-like particles
Journal
Microbiology and immunology
ISSN: 1348-0421
Titre abrégé: Microbiol Immunol
Pays: Australia
ID NLM: 7703966
Informations de publication
Date de publication:
27 Feb 2024
27 Feb 2024
Historique:
revised:
31
01
2024
received:
13
10
2023
accepted:
06
02
2024
medline:
28
2
2024
pubmed:
28
2
2024
entrez:
28
2
2024
Statut:
aheadofprint
Résumé
Immunological testing to detect neutralizing antibodies (NAbs) is important in measles (MV) infection control. Currently, the plaque reduction neutralization test is the only credible method for measuring actual virus NAbs; however, its feasibility is hampered by drawbacks, such as long turnaround times, low throughput, and the need for laboratory biosafety equipment. To solve these problems, we developed a simple and rapid MV-NAb detection system using lentivirus-based virus-like particles incorporated with the NanoLuc fragment peptide HiBiT comprising the MV fusion protein and hemagglutinin on their exterior surface. Overall, this simple, safe, and rapid method could be used to detect MV NAbs.
Identifiants
pubmed: 38414102
doi: 10.1111/1348-0421.13122
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Society for the Promotion of Science
Informations de copyright
© 2024 The Societies and John Wiley & Sons Australia, Ltd.
Références
Ota MOC, Badur S, Romano-Mazzotti L, Friedland LR. Impact of COVID-19 pandemic on routine immunization. Ann Med. 2021;53:2286-2297.
Anichini G, Gandolfo C, Fabrizi S, et al. Seroprevalence to measles virus after vaccination or natural infection in an adult population, in Italy. Vaccines. 2020;8:66.
Moss WJ, Scott S. WHO immunological basis for immunization series module xx: measles. 2008 Aug 26. Geneva: Switzerland. Accessed January 31, 2024. Available from: https://www.researchgate.net/profile/David-Bishai/publication/276235744_Measles_Eradication_versus_Measles_Control_An_Economic_Analysis/links/57bf1ac408aeb95224d0fa91/Measles-Eradication-versus-Measles-Control-An-Economic-Analysis.pdf
Haralambieva IH, Ovsyannikova IG, Vierkant RA, Poland GA. Development of a novel efficient fluorescence-based plaque reduction microneutralization assay for measles virus immunity. Clin Vaccine Immunol. 2008;15:1054-1059.
Miyakawa K, Jeremiah SS, Ohtake N, et al. Rapid quantitative screening assay for SARS-CoV-2 neutralizing antibodies using HiBiT-tagged virus-like particles. J Mol Cell Biol. 2020;12:987-990.
Frecha C, Costa C, Nègre D, et al. Stable transduction of quiescent T cells without induction of cycle progression by a novel lentiviral vector pseudotyped with measles virus glycoproteins. Blood. 2008;112:4843-4852.
Tahara M, Takeda M, Yanagi Y. Altered interaction of the matrix protein with the cytoplasmic tail of hemagglutinin modulates measles virus growth by affecting virus assembly and cell-cell fusion. J Virol. 2007;81:6827-6836.
Takeda M, Tahara M, Hashiguchi T, et al. A human lung carcinoma cell line supports efficient measles virus growth and syncytium formation via a SLAM- and CD46-independent mechanism. J Virol. 2007;81:12091-12096.