Hand2 Immunohistochemistry in the Diagnosis of Paragangliomas and Other Neuroendocrine Neoplasms.
GATA3
Hand2
Neuroendocrine tumors
Paraganglioma
Phox2B
dHand
Journal
Endocrine pathology
ISSN: 1559-0097
Titre abrégé: Endocr Pathol
Pays: United States
ID NLM: 9009288
Informations de publication
Date de publication:
Mar 2024
Mar 2024
Historique:
accepted:
08
02
2024
medline:
18
3
2024
pubmed:
28
2
2024
entrez:
28
2
2024
Statut:
ppublish
Résumé
Hand2 is a core transcription factor responsible for chromaffin cell differentiation. However, its potential utility in surgical pathology has not been studied. Thus, we aimed to investigate its expression in paragangliomas, other neuroendocrine neoplasms (NENs), and additional non-neuroendocrine tumors. We calibrated Hand2 immunohistochemistry on adrenal medulla cells and analyzed H-scores in 46 paragangliomas (PGs), 9 metastatic PGs, 21 cauda equina neuroendocrine tumors (CENETs), 48 neuroendocrine carcinomas (NECs), 8 olfactory neuroblastomas (ONBs), 110 well-differentiated NETs (WDNETs), 10 adrenal cortical carcinomas, 29 adrenal cortical adenomas, 8 melanomas, 41 different carcinomas, and 10 gastrointestinal stromal tumors (GISTs). Both tissue microarrays (TMAs) and whole sections (WSs) were studied. In 171 NENs, previously published data on Phox2B and GATA3 were correlated with Hand2. Hand2 was positive in 98.1% (54/55) PGs, but only rarely in WDNETs (9.6%, 10/104), CENETs (9.5%, 2/21), NECs (4.2%, 2/48), or ONBs (12.5%, 1/8). Any Hand2 positivity was 98.1% sensitive and 91.7% specific for the diagnosis of PG. The Hand2 H-score was significantly higher in primary PGs compared to Hand2-positive WDNETs (median 166.3 vs. 7.5; p < 0.0001). Metastatic PGs were positive in 88.9% (8/9). No Hand2 positivity was observed in any adrenal cortical neoplasm or other non-neuroendocrine tumors, with exception of 8/10 GISTs. Parasympathetic PGs showed a higher Hand2 H-score compared to sympathetic PGs (median H-scores 280 vs. 104, p < 0.0001). Hand2 positivity in NENs serves as a reliable marker of primary and metastatic PG, since other NENs only rarely exhibit limited Hand2 positivity.
Identifiants
pubmed: 38416360
doi: 10.1007/s12022-024-09803-6
pii: 10.1007/s12022-024-09803-6
doi:
Substances chimiques
Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
14-24Subventions
Organisme : BBMRI-CZ
ID : LM2023033
Organisme : Univerzita Karlova v Praze
ID : Cooperatio DIAG
Organisme : Univerzita Karlova v Praze
ID : Cooperatio METD
Organisme : European Regional Development Fund-Project BBMRI-CZ
ID : EF16_013/0001674
Organisme : Ministerstvo Zdravotnictví Ceské Republiky
ID : conceptual development of research organization 00064165
Organisme : Ministerstvo Zdravotnictví Ceské Republiky
ID : NU23-01-00323
Organisme : Grantová Agentura České Republiky
ID : 22-07091S
Organisme : Ministerstvo Obrany České Republiky
ID : MO 1012
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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