Molecular effects of photodynamic therapy with curcumin on Leishmania major promastigotes.
Leishmania
Curcumin
Gene expression
PDT
Photodynamic therapy
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
29 Feb 2024
29 Feb 2024
Historique:
received:
12
09
2023
accepted:
05
02
2024
medline:
29
2
2024
pubmed:
29
2
2024
entrez:
28
2
2024
Statut:
epublish
Résumé
Leishmaniasis is a neglected disease mainly affecting low-income populations. Conventional treatment involves several side effects, is expensive, and, in addition, protozoa can develop resistance. Photodynamic therapy (PDT) is a promising alternative in treating the disease. PDT involves applying light at a specific wavelength to activate a photosensitive compound (photosensitizer, PS), to produce reactive oxygen species (ROS). Curcumin and its photochemical characteristics make it a good candidate for photodynamic therapy. Studies evaluating gene expression can help to understand the molecular events involved in the cell death caused by PDT. In the present study, RNA was extracted from promastigotes from the control and treated groups after applying PDT. RT-qPCR was performed to verify the expression of the putative ATPase beta subunit (ATPS), ATP synthase subunit A (F0F1), argininosuccinate synthase 1 (ASS), ATP-binding cassette subfamily G member 2 (ABCG2), glycoprotein 63 (GP63), superoxide dismutase (FeSODA), and glucose-6-phosphate dehydrogenase (G6PDH) genes (QR). The results suggest that PDT altered the expression of genes that participate in oxidative stress and cell death pathways, such as ATPS, FeSODA, and G6PD. The ATP-F0F1, ASS, and GP63 genes did not have their expression altered. However, it is essential to highlight that other genes may be involved in the molecular mechanisms of oxidative stress and, consequently, in the death of parasites.
Identifiants
pubmed: 38418645
doi: 10.1007/s00436-024-08155-8
pii: 10.1007/s00436-024-08155-8
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
146Subventions
Organisme : Coordination for the Improvement of Higher Education Personnel - Brazil (CAPES)
ID : 001
Organisme : São Paulo State Research Support Foundation (FAPESP)
ID : 2016/12211-4
Organisme : São Paulo State Research Support Foundation (FAPESP)
ID : 2016/12211-4
Organisme : São Paulo State Research Support Foundation (FAPESP)
ID : 2016/12211-4
Organisme : São Paulo State Research Support Foundation (FAPESP)/CEPOF
ID : 2013/07276-1
Organisme : São Paulo State Research Support Foundation (FAPESP)/CEPOF
ID : 2013/07276-1
Organisme : Financiadora de Estudos e Projetos
ID : 01.13.0275.00
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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