PSF-Radon transform algorithm: Measurement of the point-spread function from the Radon transform of the line-spread function.
Radon transform
image resolution
line spread function
microscopy
point spread function
Journal
Microscopy research and technique
ISSN: 1097-0029
Titre abrégé: Microsc Res Tech
Pays: United States
ID NLM: 9203012
Informations de publication
Date de publication:
28 Feb 2024
28 Feb 2024
Historique:
revised:
06
01
2024
received:
28
11
2023
accepted:
10
02
2024
medline:
29
2
2024
pubmed:
29
2
2024
entrez:
29
2
2024
Statut:
aheadofprint
Résumé
In this article, we present a new method called point spread function (PSF)-Radon transform algorithm. This algorithm consists on recovering the instrument PSF from the Radon transform (in the line direction axis) of the line spread function (i.e., the image of a line). We present the method and tested with synthetic images, and real images from macro lens camera and microscopy. A stand-alone program along with a tutorial is available, for any interested user, in Martinez (PSF-Radon transform algorithm, standalone program). RESEARCH HIGHLIGHTS: Determining the instrument PSF is a key issue. Precise PSF determinations are mandatory if image improvement is performed numerically by deconvolution. Much less exposure time to achieve the same performance than a measurement of the PSF from a very small bead. Does not require having to adjust the PSF by an analytical function to overcome the noise uncertainties.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Agencia Nacional de Promoción Científica y Tecnológica
ID : PICT 2020-SERIEA-00617
Organisme : Consejo Nacional de Investigaciones Científicas y Técnicas
ID : PIP 2021-2024 112202000101184
Organisme : Universidad de Buenos Aires
ID : ubacyt 2023 20020220200032BA
Organisme : Universidad de Buenos Aires
ID : PIDAE 2022/41
Informations de copyright
© 2024 Wiley Periodicals LLC.
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