CSF N-Glycomics Using MALDI MS Techniques.

AD CSF MALDI MS MCI N-glycans

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2024
Historique:
medline: 1 3 2024
pubmed: 1 3 2024
entrez: 1 3 2024
Statut: ppublish

Résumé

In this chapter, we will present the methodology currently applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF) N-glycome. N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak

Identifiants

pubmed: 38427187
doi: 10.1007/978-1-0716-3774-6_4
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

49-65

Informations de copyright

© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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Auteurs

Angela Messina (A)

CNR, Istituto per i Polimeri, Compositi e i Biomateriali Catania, Catania, Italy.

Donata Agata Romeo (DA)

CNR, Istituto per i Polimeri, Compositi e i Biomateriali Catania, Catania, Italy.

Rita Barone (R)

CNR, Istituto per i Polimeri, Compositi e i Biomateriali Catania, Catania, Italy.
Pediatric Neurology Unit, Department of Pediatrics, University of Catania, Catania, Italy.

Luisa Sturiale (L)

CNR, Istituto per i Polimeri, Compositi e i Biomateriali Catania, Catania, Italy.

Angelo Palmigiano (A)

CNR, Istituto per i Polimeri, Compositi e i Biomateriali Catania, Catania, Italy.

Mario Zappia (M)

Section of Neurosciences-Department GF Ingrassia, University of Catania, Catania, Italy.

Domenico Garozzo (D)

CNR, Istituto per i Polimeri, Compositi e i Biomateriali Catania, Catania, Italy. domenico.garozzo@cnr.it.

Classifications MeSH