Acremonium sp. diglycosidase-aid chemical diversification: valorization of industry by-products.
Glycosylation/hydrolysis selectivity
Polyalcohols
Substrate specificity
Transglycosylation
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
02 Mar 2024
02 Mar 2024
Historique:
received:
22
08
2023
accepted:
29
11
2023
revised:
28
11
2023
medline:
2
3
2024
pubmed:
2
3
2024
entrez:
2
3
2024
Statut:
epublish
Résumé
The fungal diglycosidase α-rhamnosyl-β-glucosidase I (αRβG I) from Acremonium sp. DSM 24697 catalyzes the glycosylation of various OH-acceptors using the citrus flavanone hesperidin. We successfully applied a one-pot biocatalysis process to synthesize 4-methylumbellipheryl rutinoside (4-MUR) and glyceryl rutinoside using a citrus peel residue as sugar donor. This residue, which contained 3.5 % [w/w] hesperidin, is the remaining of citrus processing after producing orange juice, essential oil, and peel-juice. The low-cost compound glycerol was utilized in the synthesis of glyceryl rutinoside. We implemented a simple method for the obtention of glyceryl rutinoside with 99 % yield, and its purification involving activated charcoal, which also facilitated the recovery of the by-product hesperetin through liquid-liquid extraction. This process presents a promising alternative for biorefinery operations, highlighting the valuable role of αRβG I in valorizing glycerol and agricultural by-products. KEYPOINTS: • αRβG I catalyzed the synthesis of rutinosides using a suspension of OPW as sugar donor. • The glycosylation of aliphatic polyalcohols by the αRβG I resulted in products bearing a single rutinose moiety. • αRβG I catalyzed the synthesis of glyceryl rutinoside with high glycosylation/hydrolysis selectivity (99 % yield).
Identifiants
pubmed: 38430417
doi: 10.1007/s00253-023-12957-8
pii: 10.1007/s00253-023-12957-8
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
250Informations de copyright
© 2024. The Author(s).
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