A synthetic flavonoid derivate in the plasma membrane transforms the voltage-clamp fluorometry signal of CiHv1.
CiHv1
flavonoid derivate
fluorescence-quenching
membrane fluidity
voltage-clamp fluorometry
Journal
The FEBS journal
ISSN: 1742-4658
Titre abrégé: FEBS J
Pays: England
ID NLM: 101229646
Informations de publication
Date de publication:
02 Mar 2024
02 Mar 2024
Historique:
revised:
28
12
2023
received:
26
08
2023
accepted:
19
02
2024
medline:
3
3
2024
pubmed:
3
3
2024
entrez:
3
3
2024
Statut:
aheadofprint
Résumé
Voltage-clamp fluorometry (VCF) enables the study of voltage-sensitive proteins through fluorescent labeling accompanied by ionic current measurements for voltage-gated ion channels. The heterogeneity of the fluorescent signal represents a significant challenge in VCF. The VCF signal depends on where the cysteine mutation is incorporated, making it difficult to compare data among different mutations and different studies and standardize their interpretation. We have recently shown that the VCF signal originates from quenching amino acids in the vicinity of the attached fluorophores, together with the effect of the lipid microenvironment. Based on these, we performed experiments to test the hypothesis that the VCF signal could be altered by amphiphilic quenching molecules in the cell membrane. Here we show that a phenylalanine-conjugated flavonoid (4-oxo-2-phenyl-4H-chromene-7-yl)-phenylalanine, (later Oxophench) has potent effects on the VCF signals of the Ciona intestinalis H
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Slovenian Research Agency
ID : P1-0208
Organisme : János Bolyai Research Scholarship of the Hungarian Academy of Sciences
ID : BO/00355/21/8
Organisme : 2019-2.1.11-TÉT
ID : 2019-00059
Organisme : OTKA Bridging Fund
ID : 1G3DBKB0BFPF247
Organisme : OTKA
ID : 132906
Organisme : New National Excellence Program of the Ministry for Innovation and Technology
ID : ÚNKP-22-5-DE-420
Informations de copyright
© 2024 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
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