3D printing of gellan-dextran methacrylate IPNs in glycerol and their bioadhesion by RGD derivatives.
3D printing
IPN
RGD
dextran methacrylate
gellan
tissue engineering
Journal
Journal of biomedical materials research. Part A
ISSN: 1552-4965
Titre abrégé: J Biomed Mater Res A
Pays: United States
ID NLM: 101234237
Informations de publication
Date de publication:
03 Mar 2024
03 Mar 2024
Historique:
revised:
14
02
2024
received:
22
11
2023
accepted:
22
02
2024
medline:
4
3
2024
pubmed:
4
3
2024
entrez:
4
3
2024
Statut:
aheadofprint
Résumé
The ever-growing need for new tissue and organ replacement approaches paved the way for tissue engineering. Successful tissue regeneration requires an appropriate scaffold, which allows cell adhesion and provides mechanical support during tissue repair. In this light, an interpenetrating polymer network (IPN) system based on biocompatible polysaccharides, dextran (Dex) and gellan (Ge), was designed and proposed as a surface that facilitates cell adhesion in tissue engineering applications. The new matrix was developed in glycerol, an unconventional solvent, before the chemical functionalization of the polymer backbone, which provides the system with enhanced properties, such as increased stiffness and bioadhesiveness. Dex was modified introducing methacrylic groups, which are known to be sensitive to UV light. At the same time, Ge was functionalized with RGD moieties, known as promoters for cell adhesion. The printability of the systems was evaluated by exploiting the ability of glycerol to act as a co-initiator in the process, speeding up the kinetics of crosslinking. Following semi-IPNs formation, the solvent was removed by extensive solvent exchange with HEPES and CaCl
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : "Ricerche Ateneo" RM12117A81AEF242 and the CY Initiative (grant "Investissements d'avenir" ANR-16-IDEX-0008)
Informations de copyright
© 2024 Wiley Periodicals LLC.
Références
Ikada Y. Challenges in tissue engineering. J R Soc Interface. 2006;3:589-601. doi:10.1098/rsif.2006.0124
Langer R, Vacanti JP. Tissue engineering. Science. 1979;260(1993):920-926. doi:10.1126/science.8493529
Khademhosseini A, Langer R. A decade of progress in tissue engineering. Nat Protoc. 2016;11:1775-1781. doi:10.1038/nprot.2016.123
Patrick CW, Mikos AG, Mcintire LV. Chapter I-prospectus of tissue engineering. In: Patrick CWJ, Mikos AG, McIntire LV, Langer RS, eds. Frontiers in Tissue Engineering. Pergamon; 1998:3-11.
Derakhshanfar S, Mbeleck R, Xu K, Zhang X, Zhong W, Xing M. 3D bioprinting for biomedical devices and tissue engineering: a review of recent trends and advances. Bioact Mater. 2018;3:144-156. doi:10.1016/j.bioactmat.2017.11.008
Spector M. Biomaterials-based tissue engineering and regenerative medicine solutions to musculoskeletal problems. Swiss Med Wkly. 2006;136:293-301. doi:10.4414/smw.2006.11310
McKee TJ, Perlman G, Morris M, Komarova SV. Extracellular matrix composition of connective tissues: a systematic review and meta-analysis. Sci Rep. 2019;9:10542. doi:10.1038/s41598-019-46896-0
Chan BP, Leong KW. Scaffolding in tissue engineering: general approaches and tissue-specific considerations. Eur Spine J. 2008;17:467-479. doi:10.1007/s00586-008-0745-3
Tchobanian A, Van Oosterwyck H, Fardim P. Polysaccharides for tissue engineering: current landscape and future prospects. Carbohydr Polym. 2019;205:601-625. doi:10.1016/j.carbpol.2018.10.039
Jin M, Shi J, Zhu W, Yao H, Wang DA. Polysaccharide-based biomaterials in tissue engineering: a review. Tissue Eng Part B Rev. 2021;27:604-626. doi:10.1089/ten.teb.2020.0208
Sood A, Gupta A, Agrawal G. Recent advances in polysaccharides based biomaterials for drug delivery and tissue engineering applications. Carbohydrate Polymer Technologies and Applications. 2021;2:100067. doi:10.1016/j.carpta.2021.100067
Khan F, Ahmad SR. Polysaccharides and their derivatives for versatile tissue engineering application. Macromol Biosci. 2013;13:395-421. doi:10.1002/mabi.201200409
Nguyen KT, West JL. Photopolymerizable hydrogels for tissue engineering applications. Biomaterials. 2002;23:4307-4314. doi:10.1016/s0142-9612
Koivisto JT, Gering C, Karvinen J, et al. Mechanically biomimetic gelatin-Gellan gum hydrogels for 3D culture of beating human Cardiomyocytes. ACS Appl Mater Interfaces. 2019;11:20589-20602. doi:10.1021/acsami.8b22343
Yang Q, Peng J, Xiao H, Xu X, Qian Z. Polysaccharide hydrogels: functionalization, construction and served as scaffold for tissue engineering. Carbohydr Polym. 2022;278:118952. doi:10.1016/j.carbpol.2021.118952
Hersel U, Dahmen C, Kessler H. RGD modified polymers: biomaterials for stimulated cell adhesion and beyond. Biomaterials. 2003;24:4385-4415. doi:10.1016/S0142-9612
Ku SH, Lee JS, Park CB. Spatial control of cell adhesion and patterning through mussel-inspired surface modification by polydopamine. Langmuir. 2010;26:15104-15108. doi:10.1021/la102825p
Getzoff ED, Tainer JA, Weiner PK, et al. New perspectives in cell adhesion: RGD and integrins. Science. 1979;238(1987):491-497. doi:10.1126/science.2821619
Davidenko N, Schuster CF, Bax DV, et al. Evaluation of cell binding to collagen and gelatin: a study of the effect of 2D and 3D architecture and surface chemistry. J Mater Sci Mater Med. 2016;27:148. doi:10.1007/s10856-016-5763-9
Kolasa M, Czerczak K, Fraczyk J, et al. Evaluation of polysaccharide-peptide conjugates containing the RGD motif for potential use in muscle tissue regeneration. Materials. 2022;15. doi:10.3390/ma15186432
Simon-Yarza T, Labour MN, Aid R, Letourneur D. Channeled polysaccharide-based hydrogel reveals influence of curvature to guide endothelial cell arrangement in vessel-like structures. Mater Sci Eng. 2021;118:111369. doi:10.1016/j.msec.2020.111369
Matricardi P, Di Meo C, Coviello T, Hennink WE, Alhaique F. Interpenetrating polymer networks polysaccharide hydrogels for drug delivery and tissue engineering. Adv Drug Deliv Rev. 2013;65:1172-1187. doi:10.1016/j.addr.2013.04.002
Brigham MD, Eng M, Bick A, et al. Mechanically robust and bioadhesive collagen and Photocrosslinkable hyaluronic acid semi-interpenetrating networks. Tissue Eng Part A. 2009;15:1645-1653. doi:10.1089/ten.tea.2008.0441
Du M, Lu W, Zhang Y, Mata A, Fang Y. Natural polymer-sourced interpenetrating network hydrogels: fabrication, properties, mechanism and food applications. Trends Food Sci Technol. 2021;116:342-356. doi:10.1016/j.tifs.2021.07.031
Kim YJ, Min J. Property modulation of the alginate-based hydrogel via semi-interpenetrating polymer network (semi-IPN) with poly(vinyl alcohol). Int J Biol Macromol. 2021;193:1068-1077. doi:10.1016/j.ijbiomac.2021.11.069
Suo H, Zhang D, Yin J, Qian J, Wu ZL, Fu J. Interpenetrating polymer network hydrogels composed of chitosan and photocrosslinkable gelatin with enhanced mechanical properties for tissue engineering. Mater Sci Eng. 2018;92:612-620. doi:10.1016/j.msec.2018.07.016
Pescosolido L, Vermonden T, Malda J, et al. In situ forming IPN hydrogels of calcium alginate and dextran-HEMA for biomedical applications. Acta Biomater. 2011;7:1627-1633. doi:10.1016/j.actbio.2010.11.040
Pescosolido L, Piro T, Vermonden T, et al. Biodegradable IPNs based on oxidized alginate and dextran-HEMA for controlled release of proteins. Carbohydr Polym. 2011;86:208-213. doi:10.1016/j.carbpol.2011.04.033
Malda J, Visser J, Melchels FP, et al. 25th anniversary article: engineering hydrogels for biofabrication. Adv Mater. 2013;25:5011-5028. doi:10.1002/adma.201302042
Schwab A, Levato R, D'Este M, Piluso S, Eglin D, Malda J. Printability and shape Fidelity of bioinks in 3D bioprinting. Chem Rev. 2020;120:11028-11055. doi:10.1021/acs.chemrev.0c00084
Suo H, Zhang J, Xu M, Wang L. Low-temperature 3D printing of collagen and chitosan composite for tissue engineering. Mater Sci Eng. 2021;123:111963. doi:10.1016/j.msec.2021.111963
Zoratto N, Matassa R, Montanari E, et al. Glycerol as a green solvent for enhancing the formulation of dextran methacrylate and gellan-based semi-interpenetrating polymer networks. J Mater Sci. 2020;55:9562-9577. doi:10.1007/s10853-020-04732-1
Pescosolido L, Miatto S, Di Meo C, et al. Injectable and in situ gelling hydrogels for modified protein release. Eur Biophys J. 2010;39:903-909. doi:10.1007/s00249-009-0440-2
Pescosolido L, Schuurman W, Malda J, et al. Hyaluronic acid and dextran-based semi-IPN hydrogels as biomaterials for bioprinting. Biomacromolecules. 2011;12:1831-1838. doi:10.1021/bm200178w
Van Dijk-Wolthuis WNE, 0 Franssen P, Talsma H, Van Steenbergenj MJ, Den Bosch JJK-V, Henninkt WE. Synthesis, characterization, and polymerization of Glycidyl methacrylate Derivatized dextran. Macromolecules. 1995;28:6317-6322. doi:10.1021/ma00122a044
Pandey S, Alcaro MC, Scrima M, et al. Designed glucopeptides mimetics of myelin protein epitopes as synthetic probes for the detection of autoantibodies, biomarkers of multiple sclerosis. J Med Chem. 2012;55:10437-10447. doi:10.1021/jm301031r
Bondioli P, Della Bella L. An alternative spectrophotometric method for the determination of free glycerol in biodiesel. Eur J Lipid Sci Technol. 2005;107:153-157. doi:10.1002/ejlt.200401054
Oliveira JT, Santos TC, Martins L, et al. Gellan Gum Injectable Hydrogels for Cartilage Tissue Engineering Applications: In Vitro Studies and Preliminary In Vivo Evaluation. Tissue Eng, Part A. 2010;16:343-353. doi:10.1089/ten.tea.2009.0117
Oliveira JT, Martins L, Picciochi R, et al. Gellan gum: a new biomaterial for cartilage tissue engineering applications. J Biomed Mater Res A. 2010;93:852-863. doi:10.1002/jbm.a.32574
Stevens LR, Gilmore KJ, Wallace GG, in het Panhuis M. Tissue engineering with gellan gum. Biomater Sci. 2016;4:1276-1290. doi:10.1039/x0xx00000x
Morris ER, Nishinari K, Rinaudo M. Gelation of gellan-a review. Food Hydrocoll. 2012;28:373-411. doi:10.1016/j.foodhyd.2012.01.004
Smith AM, Shelton RM, Perrie Y, Harris JJ. An initial evaluation of gellan gum as a material for tissue engineering applications. J Biomater Appl. 2007;22:241-254. doi:10.1177/0885328207076522
Akkineni A, Ahlfeld T, Funk A, Waske A, Lode A, Gelinsky M. Highly concentrated alginate-Gellan gum composites for 3D plotting of complex tissue engineering scaffolds. Polymers (Basel). 2016;8:170. doi:10.3390/polym8050170
Muthukumar T, Song JE, Khang G. Biological role of gellan gum in improving scaffold drug delivery, cell adhesion properties for tissue engineering applications. Molecules. 2019;24(24):4514. doi:10.3390/molecules24244514
Gong JP. Why are double network hydrogels so tough? Soft Matter. 2010;6:2583-2590. doi:10.1039/b924290b
Cernencu AI, Ioniță M. The current state of the art in gellan-based printing inks in tissue engineering. Carbohydr Polym. 2023;309:120676. doi:10.1016/j.carbpol.2023.120676
Shin H, Olsen BD, Khademhosseini A. The mechanical properties and cytotoxicity of cell-laden double-network hydrogels based on photocrosslinkable gelatin and gellan gum biomacromolecules. Biomaterials. 2012;33:3143-3152. doi:10.1016/j.biomaterials.2011.12.050
Wen C, Lu L, Li X. An interpenetrating network biohydrogel of gelatin and gellan gum by using a combination of enzymatic and ionic crosslinking approaches. Polym Int. 2014;63:1643-1649. doi:10.1002/pi.4683
Wu D, Yu Y, Tan J, et al. 3D bioprinting of gellan gum and poly (ethylene glycol) diacrylate based hydrogels to produce human-scale constructs with high-fidelity. Mater Des. 2018;160:486-495. doi:10.1016/j.matdes.2018.09.040
Gong Y, Wang C, Lai RC, Su K, Zhang F, Wang DA. An improved injectable polysaccharide hydrogel: modified gellan gum for long-term cartilage regeneration in vitro. J Mater Chem. 2009;19:1968-1977. doi:10.1039/b818090c
Ferris CJ, Gilmore KJ, Wallace, M GG, In het Panhuis M. Modified gellan gum hydrogels for tissue engineering applications. Soft Matter. 2013;9:3705-3711. doi:10.1039/b000000x
Gering C, Koivisto JT, Parraga J, et al. Design of modular gellan gum hydrogel functionalized with avidin and biotinylated adhesive ligands for cell culture applications. PLoS One. 2019;14:e0221931. doi:10.1371/journal.pone.0221931
Silva NA, Cooke MJ, Tam RY, et al. The effects of peptide modified gellan gum and olfactory ensheathing glia cells on neural stem/progenitor cell fate. Biomaterials. 2012;33:6345-6354. doi:10.1016/j.biomaterials.2012.05.050
Wang C, Gong Y, Lin Y, Shen J, Wang DA. A novel gellan gel-based microcarrier for anchorage-dependent cell delivery. Acta Biomater. 2008;4:1226-1234. doi:10.1016/j.actbio.2008.03.008
Abreu CM, Lago MEL, Pires J, Reis RL, da Silva LP, Marques AP. Gellan gum-based hydrogels support the recreation of the dermal papilla microenvironment. Biomater Adv. 2023;150:213437. doi:10.1016/j.bioadv.2023.213437
Van Dijk-Wolthuis WNE, Kettenes-Van Den Bosch JJ, Van Der Kerk-Van Hoof A, Hennink WE. Reaction of dextran with Glycidyl methacrylate: an unexpected transesterification. Macromolecules. 1997;30:3411-3413. doi:10.1021/ma961764v
Fernandes BS, Carlos Pinto J, Cabral-Albuquerque ECM, Fialho RL. Free-radical polymerization of urea, acrylic acid, and glycerol in aqueous solutions. Polym Eng Sci. 2015;55:1219-1229. doi:10.1002/pen.24081
Guimarães CF, Gasperini L, Marques AP, Reis RL. The stiffness of living tissues and its implications for their engineering. Nat Rev Mater. 2020;5:351-370. doi:10.1038/s41578-019-0169-1
Wiebe JP, Dinsdale CJ. Inhibition of cell proliferation by glycerol. Life Sci. 1991;48:1511-1517. doi:10.1016/0024-3205
Korponyai C, Szél E, Behány Z, et al. Effects of locally applied glycerol and xylitol on the hydration, barrier function and morphological parameters of the skin. Acta Derm Venereol. 2017;97:182-187. doi:10.2340/00015555-2493
Seshadri DR, Bianco ND, Radwan AN, Zorman CA, Bogie KM. An absorbent, flexible, transparent, and scalable substrate for wound dressings. IEEE J Transl Eng Health Med. 2022;10:1-9. doi:10.1109/JTEHM.2022.3172847
Jiang J, Tang Y, Zhu H, Wei D, Sun J, Fan H. Dual functional modification of gellan gum hydrogel by introduction of methyl methacrylate and RGD contained polypeptide. Mater Lett. 2020;264:127341. doi:10.1016/j.matlet.2020.127341
Ferris CJ, Stevens LR, Gilmore KJ, et al. Peptide modification of purified gellan gum. J Mater Chem B. 2015;3:1106-1115. doi:10.1039/c4tb01727g
Dong Y, Hassan WU, Kennedy R, et al. Performance of an in situ formed bioactive hydrogel dressing from a PEG-based hyperbranched multifunctional copolymer. Acta Biomater. 2014;10:2076-2085. doi:10.1016/j.actbio.2013.12.045
Hassan W, Dong Y, Wang W. Encapsulation and 3D culture of human adipose-derived stem cells in an in-situ crosslinked hybrid hydrogel composed of PEG-based hyperbranched copolymer and hyaluronic acid. Stem Cell Res Ther. 2013;4:32. doi:10.1186/scrt182
Bellini D, Cencetti C, Meraner J, Stoppoloni D, D'Abusco AS, Matricardi P. An in situ gelling system for bone regeneration of osteochondral defects. Eur Polym J. 2015;72:642-650. doi:10.1016/j.eurpolymj.2015.02.043
Tajvar S, Hadjizadeh A, Samandari SS. Scaffold degradation in bone tissue engineering: an overview. Int Biodeterior Biodegradation. 2023;180:105599. doi:10.1016/j.ibiod.2023.105599
Jammalamadaka U, Tappa K. Recent advances in biomaterials for 3D printing and tissue engineering. J Funct Biomater. 2018;9(1):22. doi:10.3390/jfb9010022
Zhang XN, Zheng Q, Wu ZL. Recent advances in 3D printing of tough hydrogels: a review. Compos B Eng. 2022;238:109895. doi:10.1016/j.compositesb.2022.109895
Cox WP, Merz EH. Correlation of dynamic and steady flow viscosities. Journal of Polymer Science. 1958;28:619-622. doi:10.1002/pol.1958.1202811812
Lapasin R. Rheological characterization of hydrogels. In: Matricardi P, Alhaique F, Coviello T, eds. Polysaccharide Hydrogels: Characterization and Biomedical Applications. Pan Stanford Publishing Pte. Ltd.; 2015:104-110.
The Soap Detergent Association. Glycerine: An Overview. Vol 10; 1990:16.
Ducy P, Schinke T, Karsenty G. The osteoblast: a sophisticated fibroblast under central surveillance. Science. 1979;289(2000):1501-1504. doi:10.1126/science.289.5484.1501
Grzesik WJ, Robey PG. Bone matrix RGD glycoproteins: immunolocalization and interaction with human primary osteoblastic bone cells in vitro. J Bone Miner Res. 1994;9:487-496. doi:10.1002/jbmr.5650090408
Oliver-Cervelló L, Martin-Gómez H, Mas-Moruno C. New trends in the development of multifunctional peptides to functionalize biomaterials. J Pept Sci. 2022;28:e3335. doi:10.1002/psc.3335