Oral mucosal changes in tight junction proteins in patients with oral lichen planus.
oral epithelial barrier
oral lichen planus
tight junction
transcriptome analysis
Journal
Oral diseases
ISSN: 1601-0825
Titre abrégé: Oral Dis
Pays: Denmark
ID NLM: 9508565
Informations de publication
Date de publication:
04 Mar 2024
04 Mar 2024
Historique:
revised:
30
01
2024
received:
18
11
2023
accepted:
19
02
2024
medline:
5
3
2024
pubmed:
5
3
2024
entrez:
4
3
2024
Statut:
aheadofprint
Résumé
This study aimed to investigate the expression of tight junction, its distribution pattern in oral lichen planus samples and its potential association with the severity of oral lichen planus. Cross-sectional study designs were conducted. Transcriptome sequencing was conducted using oral mucosal tissues from 22 patients with oral lichen planus and 11 healthy controls. Immunohistochemistry and quantitative reverse transcription PCR were performed to verify the expression of claudin-1, claudin-4, occludin and zonula occludens-1 in oral mucosal tissues from another 30 patients with oral lichen planus and 26 healthy controls. The relationship between tight junction protein expression and oral lichen planus severity was explored using correlation analysis. 5603 and 2475 differentially expressed genes were upregulated and downregulated respectively, in oral lichen planus tissues. KEGG analysis showed that tight junctions including CLDN1, CLDN4, OCLN and TJP1 were downregulated in oral lichen planus. Claudin-1, claudin-4, occludin and zonula occludens-1 expression was verified to be significantly lower in oral lichen planus. Furthermore, correlation analyses showed that decreased occludin expression was positively related to oral lichen planus severity. Decreased expression of TJ barrier proteins may be associated with the development of oral lichen planus.
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : National Natural Science Foundation of China
ID : 81730030
Organisme : Young People Fund of Peking University School and Hospital of Stomatology
ID : PKUSS20220103
Organisme : Program for New Clinical Techniques and Therapies of Peking University School and Hospital of Stomatology
ID : PKUSSNCT-22B11
Informations de copyright
© 2024 Wiley Periodicals LLC.
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