A single amino acid at position 31 in the N-terminus of the coat protein of cucumber mosaic virus determines its avirulence function for RCY1-conferred virus resistance.
Journal
Archives of virology
ISSN: 1432-8798
Titre abrégé: Arch Virol
Pays: Austria
ID NLM: 7506870
Informations de publication
Date de publication:
05 Mar 2024
05 Mar 2024
Historique:
received:
01
09
2023
accepted:
01
12
2023
medline:
6
3
2024
pubmed:
5
3
2024
entrez:
5
3
2024
Statut:
epublish
Résumé
The coat protein (CP) of the cucumber mosaic virus (CMV) yellow strain [CMV(Y)], but not the CMV B2 strain [CMV(B2)], serves as an avirulence determinant against the NB-LRR class RCY1 of Arabidopsis thaliana. To investigate the avirulence function, a series of binary vectors were constructed by partially exchanging the CP coding sequence between CMV(Y) and CMV(B2) or introducing nucleotide substitutions. These vectors were transiently expressed in Nicotiana benthamiana leaves transformed with modified RCY1 cDNA. Analysis of hypersensitive resistance-cell death (HCD), CP accumulation, and defense gene expression at leaf sites infiltrated with Agrobacterium indicated that a single amino acid at position 31 of the CP seems to determine the avirulence function.
Identifiants
pubmed: 38441697
doi: 10.1007/s00705-024-05961-4
pii: 10.1007/s00705-024-05961-4
doi:
Substances chimiques
Amino Acids
0
DNA, Complementary
0
RCY1 protein, Arabidopsis
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
61Subventions
Organisme : Japan Society for the Promotion of Science
ID : 19H02953
Organisme : Japan Society for the Promotion of Science
ID : 22KK0081
Organisme : Japan Society for the Promotion of Science
ID : 21H04721
Organisme : Japan Society for the Promotion of Science
ID : 19K22300
Organisme : Japan Society for the Promotion of Science
ID : 23K18017
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
Références
Cooley MB, Pathirana S, Wu H-J, Kachroo P, Klessig DF (2000) Members of the Arabidopsis HRT/RPP8 family of resistance genes confer resistance to both viral and oomycete pathogens. Plant Cell 12:663–676
doi: 10.1105/tpc.12.5.663
pubmed: 10810142
pmcid: 139919
Hull R (2013) Plant virology, 5th edn. Academic Press, San-Diego. ISBN: 9780123848710
Kang S-H, Qu F, Morris TJ (2015) A spectrum of HRT-dependent hypersensitive responses elicited by the 52 amino acid N-terminus of turnip crinkle virus capsid protein and its mutants. Virus Res 200:30–34
doi: 10.1016/j.virusres.2015.01.020
pubmed: 25656064
McDowell JM, Dhandaydham M, Long TA, Aarts MGM, Goff S, Holub EB, Dangl JL (1998) Intragenic recombination and diversifying selection contribute to the evolution of downy mildew resistance at the RPP8 locus of Arabidopsis. Plant Cell 10:1861–1874
doi: 10.1105/tpc.10.11.1861
pubmed: 9811794
pmcid: 143965
Palukaitis P, García-Arenal F (2019) Cucumber mosaic virus. APS Press, St. Paul. ISBN: 978-0890546093
doi: 10.1094/9780890546109
Sambrook JF, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
Sekine K-T, Ishihara T, Hase S, Kusano T, Shah J, Takahashi H (2006) Single amino acid alterations in Arabidopsis thaliana RCY1 compromise resistance to Cucumber mosaic virus, but differentially suppress hypersensitive response-like cell death. Plant Mol Biol 62:669–682
doi: 10.1007/s11103-006-9048-4
pubmed: 16941217
Sekine K-T, Kawakami S, Kubota M, Ichinose Y, Shah J, Kang H-G, Klessig DF, Takahashi H (2008) High-level expression of a virus resistance gene, RCY1 confers extreme resistance to cucumber mosaic virus in Arabidopsis thaliana. Mol Plant-Microbe Interact 21:1398–1407
doi: 10.1094/MPMI-21-11-1398
pubmed: 18842090
Smith TJ, Chase E, Schmidt T, Perry KL (2000) The structure of cucumber mosaic virus and comparison to cowpea chlorotic mottle virus. J Virol 74:7578–7586
doi: 10.1128/JVI.74.16.7578-7586.2000
pubmed: 10906212
pmcid: 112279
Takahashi H, Goto N, Ehara Y (1994) Hypersensitive response in cucumber mosaic virus-inoculated Arabidopsis thaliana. Plant J 6:369–377
doi: 10.1046/j.1365-313X.1994.06030369.x
Takahashi H, Miller J, Nozaki Y, Sukamto TM, Shah J, Hase S et al (2002) RCY1, an Arabidopsis thaliana RPP8/HRT family resistance gene, conferring resistance to cucumber mosaic virus requires salicylic acid, ethylene and a novel signal transduction mechanism. Plant J 32:655–667
doi: 10.1046/j.1365-313X.2002.01453.x
pubmed: 12472683
Takahashi H, Nakaho K, Ishihara T, Ando S, Wada T, Kanayamam Y, Asano S et al (2014) Transcriptional profile of tomato roots exhibiting Bacillus thuringiensis-induced resistance to Ralstonia solanacearum. Plant Cell Rep 33:99–110
doi: 10.1007/s00299-013-1515-1
pubmed: 24121643
Takahashi H, Shoji H, Ando S, Kanayama Y, Kusano T, Takeshita M et al (2012) RCY1-mediated resistance to cucumber mosaic virus is regulated by LRR domain-mediated interaction with CMV(Y) following degradation of RCY1. Mol Plant-Microbe Interact 25:1171–1185
doi: 10.1094/MPMI-04-12-0076-R
pubmed: 22852808
Takahashi H, Suzuki M, Natsuaki K, Shigyo T, Hino K, Teraoka T, Hosokawa D, Ehara Y (2001) Mapping the virus and host genes involved in the resistance response in cucumber mosaic virus-infected Arabidopsis thaliana. Plant Cell Physiol 42:340–347
doi: 10.1093/pcp/pce039
pubmed: 11266586
Takahashi H, Tabara M, Miyashita S, Ando S, Kawano S, Kanayama Y, Fukuhara T, Kormelink R (2022) CMV infection in Arabidopsis: a conditional mutualistic symbiont? Front Microbiol 12:770925
doi: 10.3389/fmicb.2021.770925
pubmed: 35069476
pmcid: 8776717
Tian A, Miyashita S, Ando S, Takahashi H (2020) Single amino acid substitutions in the cucumber mosaic virus 1a protein induce necrotic cell death in virus-inoculated leaves without affecting virus multiplication. Viruses 12:91
doi: 10.3390/v12010091
pubmed: 31941092
pmcid: 7019621
Zhao Y, DelGrosso L, Yigit E, Dempsey DA, Klessig DF, Wobbe KK (2000) The amino terminus of the coat protein of turnip crinkle virus is the AVR factor recognized by resistant Arabidopsis. Mol Plant Microbe Interact 13:1015–1018
doi: 10.1094/MPMI.2000.13.9.1015
pubmed: 10975658
Zhu S, Jeong RD, Venugopal SC, Lapchyk L, Navarre D et al (2011) SAG101 forms a ternary complex with EDS1 and PAD4 and is required for resistance signaling against turnip crinkle virus. PLoS Pathog 7:e1002318
doi: 10.1371/journal.ppat.1002318
pubmed: 22072959
pmcid: 3207898