The protective effects of Gamma-linolenic acid against indomethacin-induced gastric ulcer in rats.

The primary goal of the investigation was to analyze the anti-inflammatory, and antioxidant properties of Gamma-linolenic acid (GLA) on rats with Indomethacin (IND)-induced gastric ulcers. Thirty rats were divided into five groups: Control, IND (50 mg/kg, p.o.), IND pretreated with GLA 100 mg/kg (p.o. for 14 days), IND pretreated with GLA 150 mg/kg (p.o. for 14 days), and IND pretreated with omeprazole (20 mg/kg, p.o. for 14 days). The stomach tissues were examined to calculate the ulcer index, and pH, and analyze biochemical markers (PGE2, COX1, TNF-1, IL-6, and ICAM1), and oxidative stress parameters (MDA, SOD, GSH, and CAT) as well as undergo histopathological assessment. GLA 100 and 150 mg/kg showed a protective effect against IND-induced gastric damage. It reduced levels of COX1, TNF-1, IL-6, and ICAM and increased PGE2 levels. GLA also normalized antioxidant function by modulating MDA, SOD, GSH, and CAT. GLA intervention protects against IND-induced Gastric ulcers (GU) by restoring oxidant/antioxidant balance and reducing inflammation.