In-cell NMR suggests that DNA i-motif levels are strongly depleted in living human cells.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
05 Mar 2024
05 Mar 2024
Historique:
received:
10
09
2023
accepted:
13
02
2024
medline:
6
3
2024
pubmed:
6
3
2024
entrez:
5
3
2024
Statut:
epublish
Résumé
I-Motifs (iM) are non-canonical DNA structures potentially forming in the accessible, single-stranded, cytosine-rich genomic regions with regulatory roles. Chromatin, protein interactions, and intracellular properties seem to govern iM formation at sites with i-motif formation propensity (iMFPS) in human cells, yet their specific contributions remain unclear. Using in-cell NMR with oligonucleotide iMFPS models, we monitor iM-associated structural equilibria in asynchronous and cell cycle-synchronized HeLa cells at 37 °C. Our findings show that iMFPS displaying pH
Identifiants
pubmed: 38443388
doi: 10.1038/s41467-024-46221-y
pii: 10.1038/s41467-024-46221-y
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1992Subventions
Organisme : Ministerstvo Školství, Mládeže a Tělovýchovy (Ministry of Education, Youth and Sports)
ID : CZ.02.2.69/0.0/0.0/20_079/0017045
Organisme : Ministerstvo Školství, Mládeže a Tělovýchovy (Ministry of Education, Youth and Sports)
ID : CZ.02.1.01/0.0/0.0/15_003/0000477
Organisme : Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (Conseil de Recherches en Sciences Naturelles et en Génie du Canada)
ID : Discovery Grant
Organisme : Agence Nationale de la Recherche (French National Research Agency)
ID : ANR-21-CE44-0005-01
Informations de copyright
© 2024. The Author(s).
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Viskova, P., Rynes, J., Foldynova-Trantirkova, S. & Trantirek, L. Source data: In-cell NMR suggests that DNA i-motif levels are strongly depleted in living human cells figshare. Dataset. https://doi.org/10.6084/m9.figshare.25011938.v4 (2024).