Improvement in the quality and productivity of Codonopsis pilosula seedlings by dazomet soil fumigation.
Codonopsis pilosula
Dazomet
Quality
Seedlings
Yield
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
05 Mar 2024
05 Mar 2024
Historique:
received:
14
03
2023
accepted:
01
03
2024
medline:
6
3
2024
pubmed:
6
3
2024
entrez:
5
3
2024
Statut:
epublish
Résumé
Dazomet is a dry powder formulation that releases toxic gas containing methyl isothiocyanate, which controls soil-borne pests and weeds, improving crop yields when applied to moist soils. To explore the efficacy of dazomet fumigation in the cultivation of the perennial herb Codonopsis pilosula, four typical cultivars (G1, G2, W1 and TCK) in Gansu Province were selected for seedling cultivation after soil fumigation (F) by dazomet, and non-fumigated soil was used as a control (CK). The experiments took 2 years to complete. The functional diversity of the soil enzymes and microorganisms, seedling emergence and physiological characteristics, and the quality and yield of Codonopsis seedlings and Radix were assessed. The results showed that the seed emergence rate, seedling re-green rate and several antioxidant enzymatic activities improved in the treatments involving soil fumigation with dazomet, and membrane lipid peroxidation in the seedlings decreased. On average, compared with those of the respective controls, the root viability and yield of the seedlings of the tested cultivars also increased by 34.87% and 42.4%, respectively, and the incidence of root rot in the seedlings was reduced by 83.9%, compared with their respective controls. After harvest, the yield increased by 23.9%, the incidence of root rot decreased by 61.3%, increase in yield and a 61.3% reduction in incidence, and the medicinal materials were determined to be safe and residue-free. The effects of fumigation were cultivar-specific and were especially prominent in G2. Therefore, soil fumigation with dazomet could improve the quality and productivity of Codonopsis pilosula seedlings. Taken together, these findings suggest that when herbs are bred by seedling transplantation, especially cultivars of good quality but poor resistance or species with rare germplasm resources, soil fumigation provides a way to improve cultivation effectiveness and, more importantly, ensures the probability of successfully breeding the species.
Identifiants
pubmed: 38443552
doi: 10.1038/s41598-024-56093-3
pii: 10.1038/s41598-024-56093-3
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
5407Subventions
Organisme : The "Star of Innovation" project for graduate students in Gansu Province in 2023
ID : 2023CXZX-649
Organisme : the Ministry of Agriculture and Rural Affairs of the People's Republic of China Foundation for Traditional Chinese Medicinal Materials Advantaged Characteristic Industry Cluster Construction of Improved Variety Breeding
ID : 2022GSCYJQ07-33
Organisme : A Grant for a Chief Expert of Traditional Chinese Medicinal Industry in Modern Agricultural Industry System &Safety to Yuan Chen by Department of Agriculture and Rural Affairs of Gansu Province
ID : GARS-ZYC-1
Organisme : Breeding and Base Construction of New Varieties of Chinese Medicinal Materials
ID : 7010722087
Organisme : Lanzhou youth science and technology talent innovation project
ID : 2023-QN-162
Informations de copyright
© 2024. The Author(s).
Références
Chen, L. & Huang, G. L. The antiviral activity of polysaccharides and their derivatives. Int. J. Biol. Macromol. 115, 77–82 (2018).
pubmed: 29654857
doi: 10.1016/j.ijbiomac.2018.04.056
Liu, W., Lv, X., Huang, W. H., Yao, W. B. & Gao, X. D. Characterization and hypoglycemic effect of a neutral polysaccharide extracted from the residue of Codonopsis Pilosula. Carbohyd. Polym. 197, 215–226 (2018).
doi: 10.1016/j.carbpol.2018.05.067
CP Committee. Edition of Chinese Pharmacopoeia 293–294 (Chemical Industry Press, 2020).
Chai, R. J. Textual research on the herbal history of Codonopsis pilosula. Chin. J. Integr. Med. Cardio-Cerebro. Dis. 6, 43–45 (1991).
Gao, S. M. et al. Traditional uses, phytochemistry, pharmacology and toxicology of codonopsis: A review. J. Ethnopharmacol. 20, 25 (2018).
Zou, Y. F. et al. Structural features and complement fixing activity of polysaccharides from Codonopsis pilosula nannf. Var. modesta L. T. Shen roots. Carbohyd. Polym. 113, 420–429 (2014).
doi: 10.1016/j.carbpol.2014.07.036
He, J. Y., Zhu, S., Komatsu, K., Goda, Y. & Cai, S. Q. Genetic polymorphism of medicinally-used Codonopsis species in an internal transcribed spacer sequence of nuclear ribosomal DNA and its application to authenticate Codonopsis Radix. J. Nat. Mde-Tokyo. 68, 112–124 (2014).
doi: 10.1007/s11418-013-0780-1
Sun, Q. L. et al. Structural characterization of three polysaccharides from the roots of Codonopsis pilosula and their immunomodulatory effects on RAW264.7 macrophages. Int. J. Biol. Macromol. 130, 556–563 (2019).
pubmed: 30831168
doi: 10.1016/j.ijbiomac.2019.02.165
Moon, K. G. et al. Effect of organic fertilizer application on growth characteristics and saponin content in Codonopsis lanceolata. Hortic. Environ. Biotech. 59, 125–130 (2018).
doi: 10.1007/s13580-018-0013-3
Boo, H. O. et al. Codonopsis lanceolata and Nelumbo nucifera Gaertn root extracts for functional food: Metabolic profiling by MS, FTIR and fluorescence and evaluation of cytotoxicity and anti-obesity properties on 3T3-L1 cell line. Eur. Food. Res. Technol. 243, 689–700 (2017).
doi: 10.1007/s00217-016-2782-1
Liu, D. J. et al. EDEM simulation study on the performance of a mechanized ditching device for Codonopsis planting. MDPI Agr. 12, 1238–1253 (2022).
Guo, H. B., Lu, B. R., Wu, Q. H., Chen, J. K. & Zhou, T. S. Abundant genetic diversity in cultivated codonopsis pilosula populations revealed by RAPD polymorphisms. Genet. Resour. Crop Evol. 54, 917–924 (2007).
doi: 10.1007/s10722-006-9163-8
Yang, J. et al. Establishment of in vitro culture system for Codonopsis pilosula transgenic hairy roots. 3 Biotech 10, 137–145 (2010).
doi: 10.1007/s13205-020-2130-9
Zhou, X. G., Yu, G. B. & Wu, F. Z. Effects of intercropping cucumber with onion or garlic on soil enzyme activities, microbial communities and cucumber yield. Eur. J. Soil Bio. 47, 279–287 (2011).
doi: 10.1016/j.ejsobi.2011.07.001
Sainju, U. M., Lenssen, A. W., Allen, B. L., Stevens, W. B. & Jabro, J. D. Soil total carbon and nitrogen and crop yields after eight years of tillage, crop rotation, and cultural practice. Heliyon 3, e00481–e00503 (2017).
pubmed: 29322104
doi: 10.1016/j.heliyon.2017.e00481
Ling, N., Zhang, W. W., Tan, S. Y., Huang, Q. W. & Shen, Q. R. Effect of the nursery application of bioorganic fertilizer on spatial distribution of Fusarium oxysporum f. sp. niveum and its antagonistic bacterium in the rhizosphere of watermelon. Appl. Soil Ecol. 59, 13–19 (2012).
doi: 10.1016/j.apsoil.2012.05.001
Yao, S. R., Merwin, I. A., Abawi, G. S. & Thies, J. E. Soil fumigation and compost amendment alter soil microbial community composition but do not improve tree growth or yield in an apple replant site. Soil Biol. Biochem. 38, 587–599 (2006).
doi: 10.1016/j.soilbio.2005.06.026
Shin, J., Kathuria, A. & Lee, Y. S. Effect of hydrophilic and hydrophobic cyclodextrins on the release of encapsulated allyl isothiocyanate (AITC) and their potential application for plastic film extrusion. J. Appl. Poly. Sci. 136, 48137–48146 (2019).
doi: 10.1002/app.48137
Liu, E. T., Li, Y. Y., Hu, Y. L., Sun, C. X. & Mao, L. Q. Effects of dazomet on edaphon and growth of Malus hupehensis rehd under continuous apple cropping. Acta Ecol. Sin. 34, 847–852 (2014).
Cha, S. et al. Pre-Plant fumigation of soils for nematode control affects the seedling production and morphological properties of pine and larch seedlings. MDPI For. 10, 843–854 (2019).
Fang, W. S. et al. Responses of nitrogen-cycling microorganisms to dazomet fumigation. Front. Microbiol. 9, 2529–2542 (2018).
pubmed: 30405582
pmcid: 6206233
doi: 10.3389/fmicb.2018.02529
Prider, J. & Williams, A. Using dazomet to reduce broomrape seed banks in soils with low moisture content. Crop Prot. 59, 43–50 (2014).
doi: 10.1016/j.cropro.2014.01.013
Mao, L. G. et al. Evaluation of the combination of dimethyl disulfide and dazomet as an efficient methyl bromide alternative for cucumber production in China. J. Agric. Food Chem. 62, 4864–4869 (2014).
pubmed: 24820184
doi: 10.1021/jf501255w
Mao, L. G., Jiang, H. Y., Wang, Q. X., Yan, D. D. & Cao, A. C. Efficacy of soil fumigation with dazomet for controlling ginger bacterial wilt (Ralstonia solanacearum) in China. Crop Prot. 100, 111–116 (2017).
doi: 10.1016/j.cropro.2017.06.013
Harris, D. C. A comparison of dazomet, chloropicrin and methyl bromide as soil disinfestants for strawberries. J. Hortic. Sci. 66, 51–58 (1991).
doi: 10.1080/00221589.1991.11516124
Gilreath, J. P. & Santos, B. M. Efficacy of methyl bromide alternatives on purple nutsedge (Cyperus rotundus) control in tomato and pepper. Weed Technol. 18, 341–345 (2004).
doi: 10.1614/WT-03-086R2
Eo, J. & Park, K. C. Effects of dazomet on soil organisms and recolonisation of fumigated soil. Pedobiologia 57, 147–154 (2014).
doi: 10.1016/j.pedobi.2014.01.008
He, J. Y. et al. The genus Codonopsis (Campanulaceae): A review of phytochemistry, bioactivity and quality control. J. Nat. Med. 69, 1–21 (2014).
pubmed: 25099952
pmcid: 4287686
doi: 10.1007/s11418-014-0861-9
Liu, Y., Ren, X. X. & Jeong, B. R. Manipulating the difference between the day and night temperatures can enhance the quality of Astragalus membranaceus and Codonopsis lanceolata plug seedlings. MDPI Agr. 9, 654–668 (2019).
Xiao, S. X. et al. Effects of different seedling grades on yield and commercial quality of Codonopsis pilosula. Agric. Biotech. 9, 18–21 (2020).
Bai, B. et al. Summarization of research on seedling raising technology of Codonopsis pilosula. Gansu Agric. Sci. Technol. 9, 69–73 (2018).
Jin, X. S. et al. Control effect and safety of dazomet 98% MG against weeds in Ginseng field. Agrochemistry 57, 682–686 (2018).
People’s Republic of China. Technical code of practice for dazomet soil disinfestation. NY/T 3129-2017.
Guan, S. Y. Soil Enzymes and Their Research Methods (Agricultural Press, 1986).
Zak, J. C., Willig, M. R., Moorhead, D. L. & Wildman, H. G. Functional diversity of microbial communities: A quantitative approach. Soil Biol. Biochem. 26, 1101–1108 (1994).
doi: 10.1016/0038-0717(94)90131-7
Garland, J. L. & Mills, A. L. Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization. Appl. Environ. Microbiol. 57, 2351–2359 (1991).
pubmed: 16348543
pmcid: 183575
doi: 10.1128/aem.57.8.2351-2359.1991
Garland, J. L. Analysis and interpretation of community-level physiological profiles in microbial ecology. FEMS Microbiol. Ecol. 24, 289–300 (1997).
doi: 10.1111/j.1574-6941.1997.tb00446.x
Dalal, M. & Khanna-Chopra, R. Lipid peroxidation is an early event in necrosis of wheat hybrid. Biochem. Bioph. Res. Co. 262, 109–112 (1999).
doi: 10.1006/bbrc.1999.1149
Dhindsa, R. S. & Wandekayi, M. Drought tolerance in two mosses: Correlated with enzymatic defence against lipid peroxidation. J. Exp. Bot. 1, 79–91 (1981).
doi: 10.1093/jxb/32.1.79
Li, B. L. & Mei, H. S. Relationship between oat leaf senescence and activated oxygen metabolism. Acta Phytol. Sin. 15, 6–12 (1989).
Guo, F. X. et al. Relation of several antioxidant enzymes to rapid freezing resistance in suspension cultured cells from alpine Chorispora bungeana. Cryobiology 52, 241–250 (2006).
pubmed: 16426599
doi: 10.1016/j.cryobiol.2005.12.001
Guo, F. X. et al. Initiation of early bolting by pre-enhancing anthocyanin and catalase activity in Angelica sinensis tender leaf during medicine formation cultivation year. Russ. J. Plant Physl. 68, 763–773 (2021).
doi: 10.1134/S1021443721040063
Niu, J. et al. The compensation effects of physiology and yield in cotton after drought stress. J. Plant Physl. S0176–1617(18), 30039–30047 (2018).
Inspection and Quarantine of the People's Republic of China. Industry standard for entry-exit inspection and quarantine of the People's Republic of China: Determination of crude polysaccharides in vegetable foods for export—Phenol-sulfuric acid method: SNT 4260–2015.
Yang, D. D. et al. Comparative analysis of chemical composition, antioxidant and antimicrobial activities of leaves, leaf tea and root from Codonopsis pilosula. Ind. Crop. Prod. 142, 111844–111852 (2019).
doi: 10.1016/j.indcrop.2019.111844
Gao, S. M. et al. Quantitative and HPLC fingerprint analysis combined with chemometrics for quality evaluation of Codonopsis Radix processed with different methods. Chin. Herb. Med. 2, 160–168 (2019).
Feng, Y. Z., Liu, Y. & Liu, W. Analytical method of dazomet and its metabolite methyl-isothiocyanate residue in tomato and soil. Agrochemicals 54, 588–590 (2015).
Jin, Y. B. et al. Effect of various crop residues on growth and disease resistance of Angelica sinesis seedlings in Min County. Acta Pratacul. Sin. 27, 69–78 (2018).
Qiu, X. Q. et al. Temporal and spatial distribution of root morphology of winter wheat. Sci. Agric. Sin. 46, 2211–2219 (2013).
Benlloch-Gonzalez, M., Berger, J., Bramley, H., Rebetzke, G. & Palta, J. A. The plasticity of the growth and proliferation of wheat root system under elevated CO
doi: 10.1007/s11104-013-1934-3
Wang, Z. H. et al. Effects of biochar on the microenvironment of saline-sodic soil and maize growth. MDPI Agric. 12, 2859–2876 (2022).
Sun, J. L. et al. Biochar and nitrogen fertilizer promote rice yield by altering soil enzyme activity and microbial community structure. GCB Bioenergy 14, 1266–1280 (2022).
doi: 10.1111/gcbb.12995
Wang, G., Ren, Y., Bai, X. J., Su, Y. Y. & Han, J. P. Contributions of beneficial microorganisms in soil remediation and quality improvement of medicinal plants. MDPI Plants 11, 3200–3232 (2022).
doi: 10.3390/plants11233200
Bai, G., Guo, F. X., Chen, Y., Yuan, H. C. & Xiao, W. J. Differences in physiological resistance traits of Angelica sinessis seedlings from uncultivated and cultivated fields in Min County. Acta Pratacul. Sin. 28, 86–95 (2019).
Wang, H. Y. et al. Effects of dazomet soil fumigation on characteristics of weed community in Codonopsis pilosula seedling cultivated fields. Chin. J. Eco-Agric. 29, 1827–1837 (2021).
Nicola, L. et al. Fumigation with dazomet modifies soil microbiota in apple orchards affected by replant disease. Appl. Soil Ecol. 113, 71–79 (2017).
doi: 10.1016/j.apsoil.2017.02.002
Srivastava, R. K., Pandey, P., Rajpoot, R., Rani, A. & Dubey, R. S. Cadmium and lead interactive effects on oxidative stress and antioxidative responses in rice seedlings. Protoplasma 251, 1047–1065 (2014).
pubmed: 24482190
doi: 10.1007/s00709-014-0614-3
Kanazawa, S., Sano, S., Koshiba, T. & Ushimaru, T. Changes in antioxidative enzymes in cucumber cotyledons during natural senescence: Comparison with those during dark-induced senescence. Physiol. Plant. 109, 211–216 (2000).
doi: 10.1034/j.1399-3054.2000.100214.x
Zhu, J. K. Abiotic stress signaling and responses in plants. Cell 167, 313–324 (2016).
pubmed: 27716505
pmcid: 5104190
doi: 10.1016/j.cell.2016.08.029
Jiang, W. T. et al. Effects of dazomet fumigation on growth, biological characteristics of Malus hupehensis seedlings and soil environment. Chin. J. Appl. Ecol. 31, 3085–3092 (2020).
Xu, B. Q., Chen, Y., Guo, F. X., Bai, D. T. & Zhou, D. X. Effect of transplanting densities on growth, development, yield and quality of Astragalus membranaceus var. mongholicus. Chin. J. Exp. Tra. Med. Formul. 26, 135–143 (2020).
Wu, H. M. & Lin, W. X. A commentary and development perspective on the consecutive monoculture problems of medicinal plants. Chin. J. Eco-Agric. 28, 775–793 (2020).
Fang, W. S. et al. Comparative analysis of the effects of five soil fumigants on the abundance of denitrifying microbes and changes in bacterial community composition. Ecotox. Environ. Saf. 187, 109850–109860 (2020).
doi: 10.1016/j.ecoenv.2019.109850
Cai, Z. P., Wang, H. X., Wang, G. X., Mi, Y. W. & Wu, W. G. Effects of different seedling raising modes on the quality of Codonopsis pilosula seedlings in Weiyuan. Gansu. J. Chin. Med. Mater. 40, 779–781 (2017).