Fecal microbiota impacts development of Cryptosporidium parvum in the mouse.

Journal

Scientific reports

ISSN: 2045-2322

Titre abrégé: Sci Rep

Pays: England

ID NLM: 101563288

Informations de publication

Date de publication:
06 Mar 2024

Historique:

received: 30 08 2023

accepted: 03 03 2024

medline: 7 3 2024

pubmed: 7 3 2024

entrez: 6 3 2024

Statut: epublish

Résumé

The dependence of Cryptosporidium parasites on host cell metabolites suggests that the development of nutritional interventions to limit parasite proliferation should be feasible. Based on this concept, we are testing dietary interventions to affect the enterocytes' metabolism in a manner that limits intracellular multiplication of the parasite. We hypothesize that changes in the metabolic pathways encoded by the gastro-intestinal tract microbiota may restrict parasite proliferation. To identify taxonomic and metabolic features of the microbiota associated with severity of cryptosporidiosis, as determined by estimating oocyst output, we characterized the fecal microbiota from mice experimentally infected with Cryptosporidium parvum. To eliminate the confounding effect of the interaction between co-housed mice, as well as facilitate the identification of microbiota markers associated with severity of cryptosporidiosis, fecal microbiota from individually caged mice were analyzed. Variation partitioning analysis applied to 16S sequence data from 25 mice belonging to four experiments shows that experiment was by far the biggest source of microbiota variation. Severity of cryptosporidiosis explained a smaller, though significant, fraction of microbiota variation. Notably, this effect was significant in the pre-patent phase of the infection, before mice excreted oocysts. These results are consistent with the pre-patent intestinal microbiota having a modest, but measurable, effect on cryptosporidiosis.

Identifiants

DOI: 10.1038/s41598-024-56184-1 PMID: 38448682

pubmed: 38448682

doi: 10.1038/s41598-024-56184-1

pii: 10.1038/s41598-024-56184-1

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

5498

Subventions

Organisme : National Institute of Allergy and Infectious Diseases

ID : 1R21AI144521

Informations de copyright

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