Organotypic model of the gingiva for studying bacterial and viral pathogens implicated in periodontitis.
Organotypic
Porphyromonas gingivalis
biofilm
gingival model
gingival tissue
herpes simplex virus-1
infection
persistence
Journal
Journal of oral microbiology
ISSN: 2000-2297
Titre abrégé: J Oral Microbiol
Pays: United States
ID NLM: 101551049
Informations de publication
Date de publication:
2024
2024
Historique:
medline:
8
3
2024
pubmed:
8
3
2024
entrez:
8
3
2024
Statut:
epublish
Résumé
Three-dimensional (3D) tissue models bridge the gap between conventional two-dimensional cell cultures and animal models. The aim of this study was to develop an organotypic 3D gingival (OTG) model to provide a tool to investigate bacterial and viral pathogens in periodontitis. The OTG model composed of gingival fibroblasts (GFs) and telomerase-immortalized gingival keratinocytes (TIGKs) was constructed and applied to study infections by The OTG model resembled the morphology of the human gingiva. During infection, both pathogens penetrated deep into the tissue and persisted for a few days with Presented OTG model constitutes a simple and convenient tool to study the interaction between bacterial and viral pathogens within the gingival tissue, including penetration, persistence and biofilm formation. It is also suitable to examine the efficiency of antimicrobial drugs.
Sections du résumé
Background
UNASSIGNED
Three-dimensional (3D) tissue models bridge the gap between conventional two-dimensional cell cultures and animal models. The aim of this study was to develop an organotypic 3D gingival (OTG) model to provide a tool to investigate bacterial and viral pathogens in periodontitis.
Methods
UNASSIGNED
The OTG model composed of gingival fibroblasts (GFs) and telomerase-immortalized gingival keratinocytes (TIGKs) was constructed and applied to study infections by
Results
UNASSIGNED
The OTG model resembled the morphology of the human gingiva. During infection, both pathogens penetrated deep into the tissue and persisted for a few days with
Conclusions
UNASSIGNED
Presented OTG model constitutes a simple and convenient tool to study the interaction between bacterial and viral pathogens within the gingival tissue, including penetration, persistence and biofilm formation. It is also suitable to examine the efficiency of antimicrobial drugs.
Identifiants
pubmed: 38456121
doi: 10.1080/20002297.2023.2292382
pii: 2292382
pmc: PMC10919308
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2292382Informations de copyright
© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
Déclaration de conflit d'intérêts
No potential conflict of interest was reported by the author(s).