Detection of Mycobacterium tuberculosis DNA in CD34

CD34(+) cells. IGRA Mycobacterium tuberculosis Tuberculosis infection ddPCR tuberculosis niche

Journal

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
ISSN: 1878-3511
Titre abrégé: Int J Infect Dis
Pays: Canada
ID NLM: 9610933

Informations de publication

Date de publication:
06 Mar 2024
Historique:
received: 25 01 2024
revised: 22 02 2024
accepted: 04 03 2024
medline: 9 3 2024
pubmed: 9 3 2024
entrez: 8 3 2024
Statut: aheadofprint

Résumé

To investigate whether Mycobacterium tuberculosis (Mtb) DNA is detected in peripheral blood mononuclear cells (PBMC) of subjects with tuberculosis (TB) or TB infection (TBI) living in a low TB burden country. We prospectively enrolled 57 TB-patients, 41 TBI-subjects and 39 controls in Rome, Italy. PBMC were isolated, CD34 We detected Mtb DNA at low copy number in CD34 Mtb DNA was detected at low frequency and levels in PBMC of TBI-subjects and TB-donors living in a low TB burden country. In particular, Mtb DNA was detected more frequently in CD34

Identifiants

DOI: 10.1016/j.ijid.2024.106999 PMID: 38458427
pubmed: 38458427
pii: S1201-9712(24)00070-5
doi: 10.1016/j.ijid.2024.106999
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

106999

Informations de copyright

Copyright © 2024. Published by Elsevier Ltd.

Déclaration de conflit d'intérêts

Declaration of competing interest DG reported the following conflict of interest: PBD Biotech. EG reported the following conflict of interest: Research Grants from Gilead Sciences and Mylan, not related to the presents work; Speakers fees for Gilead Sciences and ViiV, not related to this work. The remaining authors have no competing interests to declare.

Auteurs

Federica Repele (F)

Translational Research Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy.

Tonino Alonzi (T)

Translational Research Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy.

Assunta Navarra (A)

Department of Epidemiology, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy.

Chiara Farroni (C)

Translational Research Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy.

Andrea Salmi (A)

Translational Research Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy.

Gilda Cuzzi (G)

Translational Research Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy.

Giovanni Delogu (G)

Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie-Sezione di Microbiologia, Università Cattolica del Sacro Cuore, 00168, Rome, Italy; Mater Olbia Hospital, 07026, Olbia, Italy.

Gina Gualano (G)

Respiratory Infectious Diseases Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy.

Vincenzo Puro (V)

Department of Epidemiology, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy.

Gabriella De Carli (G)

Department of Epidemiology, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy.

Enrico Girardi (E)

Scientific Direction, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy.

Fabrizio Palmieri (F)

Respiratory Infectious Diseases Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy.

Adrian R Martineau (AR)

Centre for Immunobiology, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.

Delia Goletti (D)

Translational Research Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy. Electronic address: delia.goletti@inmi.it.

Classifications MeSH