Identification and pathogenicity of Fusarium species associated with wilting and crown rot in almond (Prunus dulcis).

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
08 Mar 2024
Historique:
received: 14 10 2023
accepted: 05 03 2024
medline: 9 3 2024
pubmed: 9 3 2024
entrez: 8 3 2024
Statut: epublish

Résumé

Severe Fusarium wilt and crown root symptoms were observed in almond orchards in Portugal. The present study elucidates the etiology of the disease through molecular, phenotypic, and pathogenic characterization. Three Fusarium isolates from Portugal were tested and 12 Fusarium isolates from almond from Spain were included for comparative purposes. Their identity was inferred by phylogenetic analysis combining tef1 and rpb2 sequences. The Portuguese isolates were identified as Fusarium oxysporum sensu stricto (s.s.), and the Spanish isolates as Fusarium nirenbergiae, F. oxysporum (s.s.), Fusarium proliferatum, Fusarium redolens (s.s.), Fusarium sambucinum (s.s.), and Fusarium sp. Fungal colonies and conidia were characterized on potato dextrose agar (PDA) and on Synthetischer Nährstoffarmer agar, respectively. The colonies had a variable morphology and their color ranged from white to pale violet. Typical Fusarium micro- and macroconidia were characterized. Temperature effect on mycelial growth was evaluated on PDA from 5 to 35 °C, with optimal growth temperature ranging between 16.8 and 26.4 °C. The pathogenicity of F. oxysporum was demonstrated by inoculating almond plants ('Lauranne') grafted on GF-677 or Rootpac 20 rootstocks. A significant reduction in plant growth, wilting, and xylem discoloration was observed, with Rootpac 20 being more susceptible than GF-677. Infections were also reproduced using naturally infested soils. Almond plants ('Lauranne') were inoculated with isolates of all Fusarium species, with F. redolens from Spain and F. oxysporum from Portugal being the most aggressive.

Identifiants

DOI: 10.1038/s41598-024-56350-5 PMID: 38459251
pubmed: 38459251
doi: 10.1038/s41598-024-56350-5
pii: 10.1038/s41598-024-56350-5
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

5720

Subventions

Organisme : Junta de Andalucía
ID : PP.TRA.TRA2019.002
Organisme : Junta de Andalucía
ID : ProyExcel_00327
Organisme : Agencia Estatal de Investigación
ID : CEX2019-000968-M

Informations de copyright

© 2024. The Author(s).

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Auteurs

Ana López-Moral (A)

Departamento de Agronomía (Unit of Excellence 'María de Maeztu' 2020-2024), ETSIAM, Universidad de Córdoba, Campus de Rabanales, Edif. C4, 14071, Córdoba, Spain.

Begoña Isabel Antón-Domínguez (BI)

Departamento de Agronomía (Unit of Excellence 'María de Maeztu' 2020-2024), ETSIAM, Universidad de Córdoba, Campus de Rabanales, Edif. C4, 14071, Córdoba, Spain.

María Lovera (M)

Departamento de Fruticultura Mediterránea, IFAPA, Alameda del Obispo, 14004, Córdoba, Spain.

Octavio Arquero (O)

Departamento de Fruticultura Mediterránea, IFAPA, Alameda del Obispo, 14004, Córdoba, Spain.

Antonio Trapero (A)

Departamento de Agronomía (Unit of Excellence 'María de Maeztu' 2020-2024), ETSIAM, Universidad de Córdoba, Campus de Rabanales, Edif. C4, 14071, Córdoba, Spain.

Carlos Agustí-Brisach (C)

Departamento de Agronomía (Unit of Excellence 'María de Maeztu' 2020-2024), ETSIAM, Universidad de Córdoba, Campus de Rabanales, Edif. C4, 14071, Córdoba, Spain. cagusti@uco.es.

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