Strategies for Inducing and Validating Zinc Deficiency and Zinc Repletion.

Dietary approach Mouse Model Zinc Deficiency Zinc Repletion

Journal

bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187

Informations de publication

Date de publication:
10 Mar 2024
Historique:
pubmed: 11 3 2024
medline: 11 3 2024
entrez: 11 3 2024
Statut: epublish

Résumé

Given the growing interest in the role of zinc in the onset and progression of diseases, there is a crucial demand for reliable methods to modulate zinc homeostasis. Using a dietary approach, we provide validated strategies to alter whole-body zinc in mice, applicable across species. For confirmation of zinc status, animal growth rates as well as plasma and urine zinc levels were evaluated. The accessible and cost-effective methodology outlined will increase scientific rigor, ensuring reproducibility in studies exploring the impact of zinc deficiency and repletion on the onset and progression of diseases.

Identifiants

pubmed: 38463970
doi: 10.1101/2024.02.28.582542
pmc: PMC10925336
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : NIDDK NIH HHS
ID : R01 DK133698
Pays : United States
Organisme : NIDDK NIH HHS
ID : R21 DK119879
Pays : United States

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

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Auteurs

Tara-Yesomi Wenegieme (TY)

Department of Neuroscience, Cell Biology and Physiology, Wright State University, Dayton, OH 45435 USA.
Technical Contact and.

Dalia Elased (D)

Department of Neuroscience, Cell Biology and Physiology, Wright State University, Dayton, OH 45435 USA.

Kelia E McMichael (KE)

Department of Neuroscience, Cell Biology and Physiology, Wright State University, Dayton, OH 45435 USA.

Jananie Rockwood (J)

Department of Neuroscience, Cell Biology and Physiology, Wright State University, Dayton, OH 45435 USA.

Khanzada Hasrat (K)

Department of Neuroscience, Cell Biology and Physiology, Wright State University, Dayton, OH 45435 USA.

Adaku C Um (AC)

Department of Neuroscience, Cell Biology and Physiology, Wright State University, Dayton, OH 45435 USA.
Department of Pharmacology and Toxicology, Wright State University, Dayton, OH 45435 USA.
Department of Molecular Physiology and Biophysics, Vanderbilt University, 2201 West End Ave, Nashville, TN, 37235, USA.
Department of Medicine, Vanderbilt University, Nashville, TN 37232, USA.
Technical Contact and.
Lead Contact.

Andrea G Marshall (AG)

Department of Molecular Physiology and Biophysics, Vanderbilt University, 2201 West End Ave, Nashville, TN, 37235, USA.

Kit Neikirk (K)

Department of Molecular Physiology and Biophysics, Vanderbilt University, 2201 West End Ave, Nashville, TN, 37235, USA.

Annet Kirabo (A)

Department of Medicine, Vanderbilt University, Nashville, TN 37232, USA.

Khalid M Elased (KM)

Department of Pharmacology and Toxicology, Wright State University, Dayton, OH 45435 USA.

Antentor Hinton (A)

Department of Molecular Physiology and Biophysics, Vanderbilt University, 2201 West End Ave, Nashville, TN, 37235, USA.

Clintoria R Williams (CR)

Department of Neuroscience, Cell Biology and Physiology, Wright State University, Dayton, OH 45435 USA.
Technical Contact and.
Lead Contact.

Classifications MeSH