ScISOr-ATAC reveals convergent and divergent splicing and chromatin specificities between matched cell types across cortical regions, evolution, and in Alzheimer's Disease.


Journal

bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187

Informations de publication

Date de publication:
28 Feb 2024
Historique:
pubmed: 11 3 2024
medline: 11 3 2024
entrez: 11 3 2024
Statut: epublish

Résumé

Multimodal measurements have become widespread in genomics, however measuring open chromatin accessibility and splicing simultaneously in frozen brain tissues remains unconquered. Hence, we devised Single-Cell-ISOform-RNA sequencing coupled with the Assay-for-Transposase-Accessible-Chromatin (ScISOr-ATAC). We utilized ScISOr-ATAC to assess whether chromatin and splicing alterations in the brain convergently affect the same cell types or divergently different ones. We applied ScISOr-ATAC to three major conditions: comparing (i) the Rhesus macaque (

Identifiants

pubmed: 38464236
doi: 10.1101/2024.02.24.581897
pmc: PMC10925193
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM135247
Pays : United States
Organisme : NIDA NIH HHS
ID : U01 DA053625
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH134391
Pays : United States
Organisme : NIMH NIH HHS
ID : RF1 MH121267
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH125956
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS123562
Pays : United States
Organisme : NIDA NIH HHS
ID : U01 DA058527
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH130197
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH125737
Pays : United States
Organisme : NIDA NIH HHS
ID : T32 DA039080
Pays : United States

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Auteurs

Wen Hu (W)

Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY, USA.
Center for Neurogenetics, Weill Cornell Medicine, New York, NY, USA.

Careen Foord (C)

Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY, USA.
Center for Neurogenetics, Weill Cornell Medicine, New York, NY, USA.

Justine Hsu (J)

Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY, USA.
Center for Neurogenetics, Weill Cornell Medicine, New York, NY, USA.

Li Fan (L)

Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY, USA.
Helen and Robert Appel Alzheimer's Disease Research Institute.

Michael J Corley (MJ)

Department of Medicine, Division of Infectious Diseases, Weill Cornell Medicine, New York, NY, USA.

Tarun N Bhatia (TN)

Department of Human Genetics, Emory University School of Medicine, Atlanta, GA, USA.

Siwei Xu (S)

Department of Computer Science, University of California, Irvine, CA, USA.

Natan Belchikov (N)

Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY, USA.
Center for Neurogenetics, Weill Cornell Medicine, New York, NY, USA.
Physiology, Biophysics & Systems Biology Program, Weill Cornell Medicine, New York, NY, USA.

Yi He (Y)

Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY, USA.
Center for Neurogenetics, Weill Cornell Medicine, New York, NY, USA.

Alina Ps Pang (AP)

Department of Medicine, Division of Infectious Diseases, Weill Cornell Medicine, New York, NY, USA.

Samantha N Lanjewar (SN)

Department of Human Genetics, Emory University School of Medicine, Atlanta, GA, USA.

Julien Jarroux (J)

Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY, USA.
Center for Neurogenetics, Weill Cornell Medicine, New York, NY, USA.

Anoushka Joglekar (A)

Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY, USA.
Center for Neurogenetics, Weill Cornell Medicine, New York, NY, USA.

Teresa A Milner (TA)

Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY, USA.

Lishomwa C Ndhlovu (LC)

Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY, USA.
Department of Medicine, Division of Infectious Diseases, Weill Cornell Medicine, New York, NY, USA.

Jing Zhang (J)

Department of Computer Science, University of California, Irvine, CA, USA.

Eduardo Butelman (E)

Neuropsychoimaging of Addiction and Related Conditions Research Program, Dept. of Psychiatry, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

Steven A Sloan (SA)

Department of Human Genetics, Emory University School of Medicine, Atlanta, GA, USA.

Virginia My Lee (VM)

Center for Neurodegenerative Disease Research, University of Pennsylvania School of Medicine, Philadelphia, PA, USA.

Li Gan (L)

Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY, USA.
Helen and Robert Appel Alzheimer's Disease Research Institute.

Hagen U Tilgner (HU)

Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY, USA.
Center for Neurogenetics, Weill Cornell Medicine, New York, NY, USA.

Classifications MeSH