Pathogenicity of Metarhizium rileyi (Hypocreales: Clavicipitaceae) against Tenebrio molitor (Coleoptera: Tenebrionidae).
Tenebrio molitor
eco-friendly insecticide
entomopathogenic fungi
glutathione S-transferase
stored grain
superoxide dismutase
Journal
Journal of basic microbiology
ISSN: 1521-4028
Titre abrégé: J Basic Microbiol
Pays: Germany
ID NLM: 8503885
Informations de publication
Date de publication:
11 Mar 2024
11 Mar 2024
Historique:
revised:
11
02
2024
received:
18
12
2023
accepted:
24
02
2024
medline:
11
3
2024
pubmed:
11
3
2024
entrez:
11
3
2024
Statut:
aheadofprint
Résumé
Tenebrio molitor L., also known as the mealworm, is a polyphagous insect pest that infests various stored grains worldwide. Both the adult and larval stages can cause significant damage to stored grains. The present study focused on isolating entomopathogenic fungi from an infected larval cadaver under environmental conditions. Fungal pathogenicity was tested on T. molitor larvae and pupae for 12 days. Entomopathogenic fungi were identified using biotechnological methods based on their morphology and the sequence of their nuclear ribosomal internal transcribed spacer (ITS). The results of the insecticidal activity indicate that the virulence of fungi varies between the larval and pupal stages. In comparison to the larval stage, the pupal stage is highly susceptible to Metarhizium rileyi, exhibiting 100% mortality rates after 12 days (lethal concentration 50 [LC
Identifiants
pubmed: 38466146
doi: 10.1002/jobm.202300744
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2300744Subventions
Organisme : Researchers Supporting Project
ID : RSP2024R230
Informations de copyright
© 2024 Wiley-VCH GmbH.
Références
Falcon WP, Naylor RL, Shankar ND. Rethinking global food demand for 2050. Popul Dev Rev. 2022;48:921-957.
Chegere MJ. Post-harvest losses reduction by small-scale maize farmers: the role of handling practices. Food Policy. 2018;77:103-115.
Fufa N, Zeleke T, Melese D, Daba T. Assessing storage insect pests and post-harvest loss of maize in major producing areas of Ethiopia. Int J Agric Sci Food Technol. 2021;7:193-198.
Debebe S. Post-harvest losses of crops and its determinants in Ethiopia: Tobit model analysis. Agriculture & Food Security. 2022;11:13.
Perumal V, Kannan S, Alford L, Pittarate S, Geedi R, Elangovan D, et al. First report on the enzymatic and immune response of Metarhizium majus bag formulated conidia against Spodoptera frugiperda: an ecofriendly microbial insecticide. Front Microbiol. 2023;14:1104079.
Perumal V, Kannan S, Alford L, Pittarate S, Mekchay S, Reddy GVP, et al. Biocontrol effect of entomopathogenic fungi Metarhizium anisopliae ethyl acetate-derived chemical molecules: an eco-friendly anti-malarial drug and insecticide. Arch Insect Biochem Physiol. 2023;114:1-19.
Perumal V, Kannan S, Pittarate S, Chinnasamy R, Krutmuang P. Essential oils from Acacia nilotica (Fabales: Fabaceae) seeds: May have insecticidal effects? Heliyon. 2023;9:e14808.
Swathy K, Parmar MK, Vivekanandhan P. Biocontrol efficacy of entomopathogenic fungi Beauveria bassiana conidia against agricultural insect pests. Environ Qual Manag. 2023. https://doi.org/10.1002/tqem.22174
Balumahendhiran K, Vivekanandhan P, Shivakumar MS. Mosquito control potential of secondary metabolites isolated from Aspergillus flavus and Aspergillus fumigatus. Biocatal Agric Biotechnol. 2019;21:101334.
Logeswaran C, Vivekanandhan P, Shivakumar MS. Chemical constituents of thermal stress induced Ganoderma applantum (Per.) secondary metabolites on larvae of Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus and histopathological effects in mosquito larvae. Biocatal Agric Biotechnol. 2019;20:101253.
Neethirajan S, Karunakaran C, Jayas DS, White NDG. Detection techniques for stored-product insects in grain. Food Control. 2007;18:157-162.
Plata-Rueda A, Martínez LC, Santos MHD, Fernandes FL, Wilcken CF, Soares MA, et al. Insecticidal activity of garlic essential oil and their constituents against the mealworm beetle, Tenebrio molitor Linnaeus (Coleoptera: Tenebrionidae). Sci Rep. 2017;7:46406.
Athanassiou CG, Kavallieratos NG, Boukouvala MC, Mavroforos ME, Kontodimas DC. Efficacy of alpha-cypermethrin and thiamethoxam against Trogoderma granarium everts (Coleoptera: Dermestidae) and Tenebrio molitor L. (Coleoptera: Tenebrionidae) on concrete. J Stored Prod Res. 2015;62:101-107.
Vivekanandhan P, Thendralmanikandan A, Kweka EJ, Mahande AM. Resistance to temephos in Anopheles stephensi larvae is associated with increased cytochrome P450 and α-esterase genes overexpression. Int J Trop Insect Sci. 2021;41:2543-2548.
Vivekanandhan P, Swathy K, Shivakumar MS. Stability of insecticidal molecule aucubin and their toxicity on Anopheles stephensi, Aedes aegypti, Culex quinquefasciatus and Artemia salina. Int J Trop Insect Sci. 2022;42:3403-3417.
Vivekanandhan P, Swathy K, Alford L, Pittarate S, Subala SPRR, Mekchay S, et al. Toxicity of Metarhizium flavoviride conidia virulence against Spodoptera litura (Lepidoptera: Noctuidae) and its impact on physiological and biochemical activities. Sci Rep. 2022;12:16775.
Vivekanandhan P, Swathy K, Murugan AC, Krutmuang P. Insecticidal efficacy of Metarhizium anisopliae derived chemical constituents against disease-vector mosquitoes. J Fungi. 2022;8:300.
Vivekanandhan P, Swathy K, Bedini S, Shivakumar MS. Bioprospecting of Metarhizium anisopliae derived crude extract: a ecofriendly insecticide against insect pest. Int J Trop Insect Sci. 2023;43:429-440.
Vivekanandhan P, Swathy K, Lucy A, Sarayut P, Patcharin K. Entomopathogenic fungi based microbial insecticides and their physiological and biochemical effects on Spodoptera frugiperda (JE Smith). Front Cell Infect Microbiol. 2023;13:1254475.
Chinnasamy R, Chinnaperumal K, Cherian T, Thamilchelvan K, Govindasamy B, Vetrivel C, et al. Eco-friendly phytofabrication of silver nanoparticles using aqueous extract of Aristolochia bracteolata Lam: its antioxidant potential, antibacterial activities against clinical pathogens and malarial larvicidal effects. Biomass Convers Bior. 2023:1-16. https://doi.org/10.1007/s13399-023-03750-8
Chinnasamy R, Govindasamy B, Venkatesh M, Magudeeswaran S, Dhanarajan A, Devarajan N, et al. Bio-efficacy of insecticidal molecule emodin against dengue, filariasis, and malaria vectors. Environ Sci Pollut Res. 2023;30:61842-61862.
Krutmuang P, Rajula J, Pittarate S, Chanbang Y, Perumal V, Alford L, et al. Biocontrol efficacy of Beauveria bassiana in combination with tobacco short stem and modified lure traps. Int J Trop Insect Sci. 2023;43:1591-1600.
Mannino G, Caradonna F, Cruciata I, Lauria A, Perrone A, Gentile C. Melatonin reduces inflammatory response in human intestinal epithelial cells stimulated by interleukin-1β. J Pineal Res. 2019;67:e12598.
Mantzoukas S, Kitsiou F, Natsiopoulos D, Eliopoulos PA. Entomopathogenic fungi: interactions and applications. Encyclopedia. 2022;2:646-656.
Shah ZA, Shahzad M. Fluctuation patterns of different developmental stages of Helicoverpa armigera (Lepidoptera: Noctuidae) on chickpea (Cicer arietinum) and their relationship with the environment. Entomol Fenn. 2005;16:201-206.
Adatia A, Johnson D, Entz S. Pathogenicity of two new isolates of Metarhizium anisopliae from Canadian soil to Melanoplus bivittatus (Orthoptera: Acrididae) and Tenebrio molitor (Coleoptera: Tenebrionidae). Can Entomol. 2010;142:128-134.
Maistrou S, Paris V, Jensen AB, Rolff J, Meyling NV, Zanchi C. A constitutively expressed antifungal peptide protects Tenebrio molitor during a natural infection by the entomopathogenic fungus Beauveria bassiana. Dev Comp Immunol. 2018;86:26-33.
Altahawi A, Ibrahim A, Eid F, Ahmed S, Mahfouz H. Efficacy of new isolates of entomopathogenic fungus, Metarhizium anisopliae (Metsch.), from Sinai Peninsula against yellow mealworm Tenebrio molitor L. (Coleoptera: Tenebrionidae) under laboratory conditions. J Plant Protect Pathol. 2020;11:687-691.
Kannan S, Vadesha N, Vivekanandhan P. Bioprospecting efficacy of biocontrol fungi Trichoderma harzianum: a suppression of fungal disease. TechRxiv. 2023. https://doi.org/10.22541/au.169640526.61583951/v1
Oreste M, Bubici G, Poliseno M, Triggiani O, Tarasco E. Pathogenicity of Beauveria bassiana (Bals.-Criv.) Vuill. and Metarhizium anisopliae (Metschn.) Sorokin against Galleria mellonella L. and Tenebrio molitor L. in laboratory assays. Redia. 2012;95:43-48.
Rodríguez-Gómez D, Loera O, Saucedo-Castañeda G, Viniegra-González G. Substrate influence on physiology and virulence of Beauveria bassiana acting on larvae and adults of Tenebrio molitor. World J Microbiol Biotechnol. 2009;25:513-518.
Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4:406-425.
Nei M, Kumar S. Molecular evolution and phylogenetics. New York, NY: Oxford University Press; 2000.
Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974;47:469-474.
Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. J Biol Chem. 1974;249:7130-7139.
Abbott WS. A method of computing the effectiveness of an insecticide. J Econ Entomol. 1925;18:265-267.
Goldsmith A, Loftin K, Steinkraus D, Szalanski A, Cleary D, Castrillo L. Isolation of Metarhizium guizhouense and Metarhizium robertsii strains from soil-exposed Amblyomma americanum (Acarina: Ixodidae) from northwest Arkansas, USA. Fla Entomol. 2021;104:205-212.
Acharya R, Lee JY, Hwang HS, Kim MK, Lee SY, Jung HY, et al. Identification of entomopathogenic fungus Metarhizium rileyi infested in fall armyworm in the cornfield of Korea, and evaluation of its virulence. Arch Insect Biochem Physiol. 2022;111:e21965.
Lingg AJ, Donaldson MD. Biotic and abiotic factors affecting stability of Beauveria bassiana conidia in soil. J Invertebr Pathol. 1981;38:191-200.
Raid RN, Cherry RH. Pathogenicity of Metarhizium anisopliae var. major (Metschnikoff) Sorokin to a sugarcane grub Ligyrus subtropicus (Blatchley) (Coleoptera: Scarabaeidae). J Agric Entomol. 1992;9:11-16.
Kilic E, Guven O, Baydar R, Karaca I. The mortality effects of some entomopathogenic fungi against Helicoverpa armigera, Spodoptera littoralis, Tenebrio molitor and Blattella germanica. Kafkas Univ Vet Fak Derg. 2019;25:33-37.
Setiawan A. Entomopathogenic fungi of soils of freshwater swamps, tidal lowlands, peatlands, and highlands of south. Biodiversity. 2018;19:2365-2373.
Simamora M, Basyuni M, Lisnawita L. Potency of secondary metabolites of Trichoderma asperellum and Pseudomonas fluorescens in the growth of cocoa plants affected by vascular streak dieback. Biodiversitas J Biol Divers. 2021;22:1-7.
Fang W, Feng J, Fan Y, Zhang Y, Bidochka MJ, Leger RJS, et al. Expressing a fusion protein with protease and chitinase activities increases the virulence of the insect pathogen Beauveria bassiana. J Invertebr Pathol. 2009;102:155-159.
Fang W, Pei Y, Bidochka MJ. A regulator of a G protein signalling (RGS) gene, cag8, from the insect-pathogenic fungus Metarhizium anisopliae is involved in conidiation, virulence and hydrophobin synthesis. Microbiology. 2007;153:1017-1025.
Dubovskiy IM, Grizanova EV, Ershova NS, Rantala MJ, Glupov VV. The effects of dietary nickel on the detoxification enzymes, innate immunity and resistance to the fungus Beauveria bassiana in the larvae of the greater wax moth Galleria mellonella. Chemosphere. 2011;85:92-96.
Kaur T, Kaur J, Kaur A, Kaur S. Larvicidal and growth inhibitory effects of endophytic Aspergillus Niger on a polyphagous pest, Spodoptera litura. Phytoparasitica. 2016;44:465-476.
González-Santoyo I, Córdoba-Aguilar A. Phenoloxidase: A key component of the insect immune system. Entomol Exp Appl. 2012;142:1-16.
Rajendran S, Vasudevan S. Activation of prophenoloxidase and hyperglycemia as indicators of microbial stress in the blue swimmer crab Portunus pelagicus. Mar Pollut Bull. 2020;160:111711.
Pedersen KE, Pedersen NN, Meyling NV, Fredensborg BL, Cedergreen N. Differences in life stage sensitivity of the beetle Tenebrio molitor towards a pyrethroid insecticide explained by stage-specific variations in uptake, elimination and activity of detoxifying enzymes. Pest Biochem Physiol. 2020;162:113-121.
Ansari MA, Butt TM. Evaluation of entomopathogenic fungi and a nematode against the soil-dwelling stages of the crane fly Tipula paludosa. Pest Manag Sci. 2012;68:1337-1344.
Nguyen NH, Suh SO, Blackwell M. Five novel Candida species in insect-associated yeast clades isolated from Neuroptera and other insects. Mycologia. 2007;99:842-858.
Anand R, Tiwary BN. Pathogenicity of entomopathogenic fungi to eggs and larvae of Spodoptera litura, the common cutworm. Biocontrol Sci Technol. 2009;19:919-929.
Beris EI, Papachristos DP, Fytrou A, Antonatos S, Kontodimas DC. Pathogenicity of three entomopathogenic fungi on pupae and adults of the Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae). J Pest Sci. 2013;86:275-284.
Rhodes VL, Thomas MB, Michel K. The interplay between dose and immune system activation determines fungal infection outcome in the African malaria mosquito, Anopheles gambiae. Dev Comp Immunol. 2018;85:125-133.
Vivekanandhan P, Swathy K, Thomas A, Kweka EJ, Rahman A, Pittarate S, et al. Insecticidal efficacy of microbial-mediated synthesized copper nano-pesticide against insect pests and non-target organisms. Int J Environ Res Public Health. 2021;18:10536.