Functional characterization and transcriptional analysis of degQ of Xanthomonas campestris pathovar campestris.
Xanthomonas
bacterial attachment
degQ
pathogenicity
stress tolerance
transcription
Journal
Journal of basic microbiology
ISSN: 1521-4028
Titre abrégé: J Basic Microbiol
Pays: Germany
ID NLM: 8503885
Informations de publication
Date de publication:
12 Mar 2024
12 Mar 2024
Historique:
revised:
07
01
2024
received:
02
08
2023
accepted:
20
01
2024
medline:
12
3
2024
pubmed:
12
3
2024
entrez:
12
3
2024
Statut:
aheadofprint
Résumé
High-temperature-requirement protein A (HtrA) family proteins play important roles in controlling protein quality and are recognized as virulence factors in numerous animal and human bacterial pathogens. The role of HtrA family proteins in plant pathogens remains largely unexplored. Here, we investigated the HtrA family protein, DegQ, in the crucifer black rot pathogen Xanthomonas campestris pathovar campestris (Xcc). DegQ is essential for bacterial attachment and full virulence of Xcc. Moreover, the degQ mutant strain showed increased sensitivity to heat treatment and sodium dodecyl sulfate. Expressing the intact degQ gene in trans in the degQ mutant could reverse the observed phenotypic changes. In addition, we demonstrated that the DegQ protein exhibited chaperone-like activity. Transcriptional analysis displayed that degQ expression was induced under heat treatment. Our results contribute to understanding the function and expression of DegQ of Xcc for the first time and provide a novel perspective about HtrA family proteins in plant pathogen.
Identifiants
pubmed: 38470163
doi: 10.1002/jobm.202300441
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202300441Subventions
Organisme : National Science and Technology Council
ID : NSTC112-2313-B-166-001
Organisme : Ministry of Science and Technology, Taiwan
ID : MOST111-2313-B-166-001
Informations de copyright
© 2024 Wiley-VCH GmbH.
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