Biodistribution of Drug/ADA Complexes: The Impact of Immune Complex Formation on Antibody Distribution.

biodistribution immune complexes pharmacokinetics radiolabeling tritium

Journal

The AAPS journal
ISSN: 1550-7416
Titre abrégé: AAPS J
Pays: United States
ID NLM: 101223209

Informations de publication

Date de publication:
13 Mar 2024
Historique:
received: 08 12 2023
accepted: 10 02 2024
medline: 13 3 2024
pubmed: 13 3 2024
entrez: 13 3 2024
Statut: epublish

Résumé

The clinical use of therapeutic monoclonal antibodies (mAbs) for the treatment of cancer, inflammation, and other indications has been successfully established. A critical aspect of drug-antibody pharmacokinetics is immunogenicity, which triggers an immune response via an anti-drug antibody (ADA) and forms drug/ADA immune complexes (ICs). As a consequence, there may be a reduced efficacy upon neutralization by ADA or an accelerated drug clearance. It is therefore important to understand immunogenicity in biological therapies. A drug-like immunoglobulin G (IgG) was radiolabeled with tritium, and ICs were formed using polyclonal ADA, directed against the complementary-determining region of the drug-IgG, to investigate in vivo biodistribution in rodents. It was demonstrated that 65% of the radioactive IC dose was excreted within the first 24 h, compared with only 6% in the control group who received non-complexed

Identifiants

pubmed: 38478197
doi: 10.1208/s12248-024-00899-6
pii: 10.1208/s12248-024-00899-6
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

33

Informations de copyright

© 2024. The Author(s).

Références

Grilo AL, Mantalaris A. The increasingly human and profitable monoclonal antibody market. Trends Biotechnol. 2019;37(1):9–16.
doi: 10.1016/j.tibtech.2018.05.014 pubmed: 29945725
Krishna M, Nadler SG. Immunogenicity to biotherapeutics–the role of anti-drug immune complexes. Front Immunol. 2016;7:21.
doi: 10.3389/fimmu.2016.00021 pubmed: 26870037 pmcid: 4735944
Stubenrauch K, Künzel C, Vogel R, Tuerck D, Schick E, Heinrich J. Epitope characterization of the ADA response directed against a targeted immunocytokine. J Pharm Biomed Anal. 2015;114:296–304.
doi: 10.1016/j.jpba.2015.05.029 pubmed: 26093509
Harding FA, Stickler MM, Razo J, DuBridge R. The immunogenicity of humanized and fully human antibodies: residual immunogenicity resides in the CDR regions. MAbs. 2010;2(3):256–65.
doi: 10.4161/mabs.2.3.11641 pubmed: 20400861 pmcid: 2881252
Cassinotti A, Ardizzone S, Porro GB. Adalimumab for the treatment of Crohn’s disease. Biologics. 2008;2(4):763.
pubmed: 19707457 pmcid: 2727899
Ponce R, Abad L, Amaravadi L, Gelzleichter T, Gore E, Green J, et al. Immunogenicity of biologically-derived therapeutics: assessment and interpretation of nonclinical safety studies. Regul Toxicol Pharmacol. 2009;54(2):164–82.
doi: 10.1016/j.yrtph.2009.03.012 pubmed: 19345250
Opolka-Hoffmann E, Jordan G, Otteneder M, Kieferle R, Lechmann M, Winter G, et al. The impact of immunogenicity on therapeutic antibody pharmacokinetics: a preclinical evaluation of the effect of immune complex formation and antibody effector function on clearance. MAbs. 2021;13(1):1995929.
doi: 10.1080/19420862.2021.1995929 pubmed: 34763611 pmcid: 8726625
Chirmule N, Jawa V, Meibohm B. Immunogenicity to therapeutic proteins: impact on PK/PD and efficacy. AAPS J. 2012;14(2):296–302.
doi: 10.1208/s12248-012-9340-y pubmed: 22407289 pmcid: 3326159
van Schie KA, Kruithof S, Ooijevaar-de Heer P, Derksen NI, van de Bovenkamp FS, Saris A, et al. Restricted immune activation and internalisation of anti-idiotype complexes between drug and antidrug antibodies. Ann Rheum Dis. 2018;77(10):1471–9.
doi: 10.1136/annrheumdis-2018-213299 pubmed: 29945923
Hoffmann E, Jordan G, Lauer M, Ringler P, Kusznir EA, Rufer AC, et al. Generation, characterization, and quantitative bioanalysis of drug/anti-drug antibody immune complexes to facilitate dedicated in vivo studies. Pharm Res. 2019;36(9):1–15.
doi: 10.1007/s11095-019-2661-0
Rojko JL, Evans MG, Price SA, Han B, Waine G, DeWitte M, et al. Formation, clearance, deposition, pathogenicity, and identification of biopharmaceutical-related immune complexes: review and case studies. Toxicol Pathol. 2014;42(4):725–64.
doi: 10.1177/0192623314526475 pubmed: 24705884
Rojas JR, Taylor RP, Cunningham MR, Rutkoski TJ, Vennarini J, Jang H, et al. Formation, distribution, and elimination of infliximab and anti-infliximab immune complexes in cynomolgus monkeys. J Pharmacol Exp Ther. 2005;313(2):578–85.
doi: 10.1124/jpet.104.079277 pubmed: 15647331
Staack RF, Stracke JO, Stubenrauch K, Vogel R, Schleypen J, Papadimitriou A. Quality requirements for critical assay reagents used in bioanalysis of therapeutic proteins: what bioanalysts should know about their reagents. Bioanalysis. 2011;3(5):523–34.
doi: 10.4155/bio.11.16 pubmed: 21388265
Edelmann MR, Hauri S. Functional in vitro assessment of modified antibodies: impact of label on protein properties. PLoS One. 2021;16(9):e0257342.
doi: 10.1371/journal.pone.0257342 pubmed: 34529712 pmcid: 8445452
Schlothauer T, Herter S, Koller CF, Grau-Richards S, Steinhart V, Spick C, et al. Novel human IgG1 and IgG4 Fc-engineered antibodies with completely abolished immune effector functions. Protein Eng Des Sel. 2016;29(10):457–66.
doi: 10.1093/protein/gzw040 pubmed: 27578889
Edelmann MR, Kettenberger H, Knaupp A, Schlothauer T, Otteneder MB. Radiolabeled IgG antibodies: impact of various labels on neonatal Fc receptor binding. J Label Compds Radiopharm. 2019;62(11):751–7.
doi: 10.1002/jlcr.3793
Wang L, Amphlett G, Blättler WA, Lambert JM, Zhang W. Structural characterization of the maytansinoid–monoclonal antibody immunoconjugate, huN901–DM1, by mass spectrometry. Protein Sci. 2005;14(9):2436–46.
doi: 10.1110/ps.051478705 pubmed: 16081651 pmcid: 2253466
Johansson A, Erlandsson A, Eriksson D, Ullén A, Holm P, Sundström BE, et al. Idiotypic–anti-idiotypic complexes and their in vivo metabolism. Cancer. 2002;94(S4):1306–13.
doi: 10.1002/cncr.10301 pubmed: 11877761
Edelmann MR. Radiolabelling small and biomolecules for tracking and monitoring. RSC Adv. 2022;12(50):32383–400.
doi: 10.1039/D2RA06236D pubmed: 36425706 pmcid: 9650631
Pastuskovas CV, Mallet W, Clark S, Kenrick M, Majidy M, Schweiger M, et al. Effect of immune complex formation on the distribution of a novel antibody to the ovarian tumor antigen CA125. Drug Metab Dispos. 2010;38(12):2309–19.
doi: 10.1124/dmd.110.034330 pubmed: 20823292
Wang M, Zhan Y, O’Neil SP, Harris S, Henson C, McEwen A, et al. Quantitative biodistribution of biotherapeutics at whole body, organ and cellular levels by autoradiography. Bioanaly. 2018;10(18):1487–500.
doi: 10.4155/bio-2018-0046
Sepp A, Meno-Tetang G, Weber A, Sanderson A, Schon O, Berges A. Computer-assembled cross-species/cross-modalities two-pore physiologically based pharmacokinetic model for biologics in mice and rats. J Pharmacokinet Pharmacodyn. 2019;46(4):339–59.
doi: 10.1007/s10928-019-09640-9 pubmed: 31079322

Auteurs

Eugenia Opolka-Hoffmann (E)

Roche Pharma Research and Early Development, Pharmaceutical Sciences, Roche Innovation Center Munich, Roche Diagnostics GmbH, Nonnenwald 2, DE-82377, Penzberg, Germany. Eugenia.opolka-hoffmann@roche.com.

Martin R Edelmann (MR)

Department of Pharmacy and Pharmacology, University of Bath, Bath, BA2 7AY, UK.
Roche Pharma Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd, Therapeutic Modalities, CH-4070, Basel, Switzerland.

Michael B Otteneder (MB)

Roche Pharma Research and Early Development, Pharmaceutical Sciences, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd, CH-4070, Basel, Switzerland.

Simon Hauri (S)

Roche Pharma Research and Early Development, Pharmaceutical Sciences, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd, CH-4070, Basel, Switzerland.

Gregor Jordan (G)

Roche Pharma Research and Early Development, Pharmaceutical Sciences, Roche Innovation Center Munich, Roche Diagnostics GmbH, Nonnenwald 2, DE-82377, Penzberg, Germany.

Peter Schrag (P)

Roche Pharma Research and Early Development, Pharmaceutical Sciences, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd, CH-4070, Basel, Switzerland.

Martin Lechmann (M)

Roche Pharma Research and Early Development, Pharmaceutical Sciences, Roche Innovation Center Munich, Roche Diagnostics GmbH, Nonnenwald 2, DE-82377, Penzberg, Germany.

Gerhard Winter (G)

Department of Pharmacy, Pharmaceutical Technology & Biopharmaceutics, Ludwig-Maximilians University, DE-80539, Munich, Germany.

Roland F Staack (RF)

Roche Pharma Research and Early Development, Pharmaceutical Sciences, Roche Innovation Center Munich, Roche Diagnostics GmbH, Nonnenwald 2, DE-82377, Penzberg, Germany.

Classifications MeSH