Promoter screening and identification for metabolic regulation in Acremonium chrysogenum.
Acremonium chrysogenum
Golden Gate assembly
bidirectional promoter
cephalosporin C
promoter library
Journal
Biotechnology journal
ISSN: 1860-7314
Titre abrégé: Biotechnol J
Pays: Germany
ID NLM: 101265833
Informations de publication
Date de publication:
Mar 2024
Mar 2024
Historique:
revised:
01
02
2024
received:
03
12
2023
accepted:
19
02
2024
medline:
14
3
2024
pubmed:
14
3
2024
entrez:
13
3
2024
Statut:
ppublish
Résumé
Acremonium chrysogenum is the major industrial producer of cephalosporin C (CPC), which is used as raw material for the production of significant cephalosporin antibiotics. Due to the lack of diverse promoter elements, the development of metabolic engineering transformation is relatively slow, resulting in a limited improvement on CPC production. In this study, based on the analysis of the transcriptome profile, 27 candidate promoters were selected to drive the expression of the reporter genes. The promoter activities of this library ranged from 0.0075 to 101 times of the control promoter P
Identifiants
pubmed: 38479986
doi: 10.1002/biot.202300683
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2300683Subventions
Organisme : National Key Research Development Program of China
ID : 2021YFC2100203
Organisme : Taishan Scholars Program
Organisme : Natural Science Foundation of Shanghai
ID : 23ZR1416500
Organisme : Frontiers Science Center for Materiobiology and Dynamic Chemistry
ID : JKVJ1231036
Informations de copyright
© 2024 Wiley-VCH GmbH.
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