In-vitro selection of lactic acid bacteria to combat Salmonella enterica and Campylobacter jejuni in broiler chickens.

Anti-Campylobacter Anti-Salmonella Foodborne pathogens and organic acids

Journal

World journal of microbiology & biotechnology

ISSN: 1573-0972

Titre abrégé: World J Microbiol Biotechnol

Pays: Germany

ID NLM: 9012472

Informations de publication

Date de publication:
14 Mar 2024

Historique:

received: 26 09 2023

accepted: 28 02 2024

medline: 14 3 2024

pubmed: 14 3 2024

entrez: 14 3 2024

Statut: epublish

Résumé

Campylobacter and Salmonella are the two most prominent foodborne zoonotic pathogens reported in the European Union. As poultry is one of the major sources of these pathogens, it is imperative to mitigate the colonization of these pathogens in poultry. Many strains of lactic acid bacteria (LAB) have demonstrated anti-Salmonella and anti-Campylobacter characteristics to varying degrees and spectrums which are attributed to the production of various metabolites. However, the production of these compounds and consequent antimicrobial properties are highly strain dependent. Therefore, the current study was performed to select a potent LAB and determine its causal attribute in inhibiting Salmonella enterica and Campylobacter jejuni, in-vitro. Six LAB (Lactiplantibacillus plantarum (LP), Lacticaseibacillus casei (LC), Limosilactobacillus reuteri (LR), Lacticaseibacillus rhamnosus (LRh), Leuconostoc mesenteroides (LM) and Pediococcus pentosaceus (PP)) and three serovars of Salmonella enterica (Typhimurium, Enterica and Braenderup) and Campylobacter jejuni were used in the current study. Spot overlays, well diffusion, co-culture and co-aggregation assays against Salmonella and well diffusion assays against Campylobacter jejuni were performed. Organic acid profiling of culture supernatants was performed using HPLC. The results indicated that LRh, LM and PP had the most significant anti-Salmonella effects while LP, LC, LM and PP displayed the most significant anti-Campylobacter effects. Lactic acid and formic acid detected in the culture supernatants seem the most likely source of the anti-Salmonella and anti-Campylobacter effects exhibited by these LAB. In conclusion, Leuconostoc mesenteroides displayed the most significant overall anti-pathogenic effects when compared to the other LAB strains studied, indicating its potential application in-vivo.

Identifiants

DOI: 10.1007/s11274-024-03946-8 PMID: 38480610

pubmed: 38480610

doi: 10.1007/s11274-024-03946-8

pii: 10.1007/s11274-024-03946-8

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

133

Subventions

Organisme : European Union's Horizon 2020 research and innovation programme

ID : Grant agreement No 955374.

Informations de copyright

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