MemPrep, a new technology for isolating organellar membranes provides fingerprints of lipid bilayer stress.
ER Stress
Lipid Bilayer Stress
MemPrep
Organelle Lipidomics
UPR
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
15 Mar 2024
15 Mar 2024
Historique:
received:
14
09
2022
accepted:
26
02
2024
revised:
16
02
2024
pubmed:
16
3
2024
medline:
16
3
2024
entrez:
16
3
2024
Statut:
aheadofprint
Résumé
Biological membranes have a stunning ability to adapt their composition in response to physiological stress and metabolic challenges. Little is known how such perturbations affect individual organelles in eukaryotic cells. Pioneering work has provided insights into the subcellular distribution of lipids in the yeast Saccharomyces cerevisiae, but the composition of the endoplasmic reticulum (ER) membrane, which also crucially regulates lipid metabolism and the unfolded protein response, remains insufficiently characterized. Here, we describe a method for purifying organelle membranes from yeast, MemPrep. We demonstrate the purity of our ER membrane preparations by proteomics, and document the general utility of MemPrep by isolating vacuolar membranes. Quantitative lipidomics establishes the lipid composition of the ER and the vacuolar membrane. Our findings provide a baseline for studying membrane protein biogenesis and have important implications for understanding the role of lipids in regulating the unfolded protein response (UPR). The combined preparative and analytical MemPrep approach uncovers dynamic remodeling of ER membranes in stressed cells and establishes distinct molecular fingerprints of lipid bilayer stress.
Identifiants
pubmed: 38491296
doi: 10.1038/s44318-024-00063-y
pii: 10.1038/s44318-024-00063-y
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Volkswagen Foundation (VolkswagenStiftung)
ID : #93089,#93092,#93090
Organisme : Deutsche Forschungsgemeinschaft (DFG)
ID : SFB1027
Organisme : Deutsche Forschungsgemeinschaft (DFG)
ID : SFB894
Organisme : EC | ERC | HORIZON EUROPE European Research Council (ERC)
ID : 866011
Informations de copyright
© 2024. The Author(s).
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