Protocol for xenografting of BxPC-3 pancreatic tumors using a chorioallantoic membrane model.
Cancer
Cell culture
Model Organisms
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
15 Mar 2024
15 Mar 2024
Historique:
received:
18
11
2023
revised:
04
01
2024
accepted:
04
03
2024
medline:
16
3
2024
pubmed:
16
3
2024
entrez:
16
3
2024
Statut:
aheadofprint
Résumé
The chorioallantoic membrane (CAM) model is an increasingly attractive model for the study of human tumors. However, concise techniques for the use of pancreatic ductal adenocarcinoma BxPC-3 xenografts in CAM assays are not yet available. Here, we present a protocol for the induction of BxPC-3 xenograft tumors with high grafting efficiency. We describe steps for embryo incubation, egg handling, and grafting, each of which has been optimized to prevent fungal contamination and minimize mortality.
Identifiants
pubmed: 38492226
pii: S2666-1667(24)00133-3
doi: 10.1016/j.xpro.2024.102968
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102968Informations de copyright
Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests This research is partly funded by a grant from ElmediX NV. T.L. and E.v.Z. are employees of ElmediX. J.B. is the CEO and a shareholder of ElmediX NV.