Granzyme B degrades extracellular matrix and promotes inflammation and choroidal neovascularization.

Age-related macular degeneration Angiogenesis Choroidal neovascularization Extracellular matrix Granzyme B Inflammation Mast cell

Journal

Angiogenesis
ISSN: 1573-7209
Titre abrégé: Angiogenesis
Pays: Germany
ID NLM: 9814575

Informations de publication

Date de publication:
18 Mar 2024
Historique:
received: 25 09 2023
accepted: 11 02 2024
medline: 19 3 2024
pubmed: 19 3 2024
entrez: 18 3 2024
Statut: aheadofprint

Résumé

Age-related macular degeneration (AMD) is a common retinal neurodegenerative disease among the elderly. Neovascular AMD (nAMD), a leading cause of AMD-related blindness, involves choroidal neovascularization (CNV), which can be suppressed by anti-angiogenic treatments. However, current CNV treatments do not work in all nAMD patients. Here we investigate a novel target for AMD. Granzyme B (GzmB) is a serine protease that promotes aging, chronic inflammation and vascular permeability through the degradation of the extracellular matrix (ECM) and tight junctions. Extracellular GzmB is increased in retina pigment epithelium (RPE) and mast cells in the choroid of the healthy aging outer retina. It is further increased in donor eyes exhibiting features of nAMD and CNV. Here, we show in RPE-choroidal explant cultures that exogenous GzmB degrades the RPE-choroid ECM, promotes retinal/choroidal inflammation and angiogenesis while diminishing anti-angiogenic factor, thrombospondin-1 (TSP-1). The pharmacological inhibition of either GzmB or mast-cell degranulation significantly reduces choroidal angiogenesis. In line with our in vitro data, GzmB-deficiency reduces the extent of laser-induced CNV lesions and the age-related deterioration of electroretinogram (ERG) responses in mice. These findings suggest that targeting GzmB, a serine protease with no known endogenous inhibitors, may be a potential novel therapeutic approach to suppress CNV in nAMD.

Identifiants

pubmed: 38498232
doi: 10.1007/s10456-024-09909-9
pii: 10.1007/s10456-024-09909-9
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NIH HHS
ID : S10RR024585
Pays : United States
Organisme : NIH HHS
ID : S10RR024585
Pays : United States

Informations de copyright

© 2024. The Author(s).

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Auteurs

Gideon Obasanmi (G)

Department of Ophthalmology and Visual Sciences, UBC, Vancouver, BC, Canada.

Manjosh Uppal (M)

Department of Ophthalmology and Visual Sciences, UBC, Vancouver, BC, Canada.

Jing Z Cui (JZ)

Department of Ophthalmology and Visual Sciences, UBC, Vancouver, BC, Canada.

Jeanne Xi (J)

Department of Ophthalmology and Visual Sciences, UBC, Vancouver, BC, Canada.

Myeong Jin Ju (MJ)

Department of Ophthalmology and Visual Sciences, UBC, Vancouver, BC, Canada.
School of Biomedical Engineering, UBC, Vancouver, BC, Canada.

Jun Song (J)

School of Biomedical Engineering, UBC, Vancouver, BC, Canada.

Eleanor To (E)

Department of Ophthalmology and Visual Sciences, UBC, Vancouver, BC, Canada.

Siqi Li (S)

Department of Ophthalmology and Visual Sciences, UBC, Vancouver, BC, Canada.

Wania Khan (W)

Department of Ophthalmology and Visual Sciences, UBC, Vancouver, BC, Canada.

Darian Cheng (D)

Department of Ophthalmology and Visual Sciences, UBC, Vancouver, BC, Canada.

John Zhu (J)

Department of Ophthalmology and Visual Sciences, UBC, Vancouver, BC, Canada.

Lyden Irani (L)

Department of Ophthalmology and Visual Sciences, UBC, Vancouver, BC, Canada.

Isa Samad (I)

Department of Ophthalmology and Visual Sciences, UBC, Vancouver, BC, Canada.

Julie Zhu (J)

Department of Ophthalmology and Visual Sciences, UBC, Vancouver, BC, Canada.

Hyung-Suk Yoo (HS)

Department of Ophthalmology and Visual Sciences, UBC, Vancouver, BC, Canada.

Alexandre Aubert (A)

International Collaboration On Repair Discoveries (ICORD), Vancouver Coastal Health Research Institute, University of British Columbia (UBC), Vancouver, BC, Canada.
Department of Pathology and Laboratory Medicine, UBC, Vancouver, BC, Canada.

Jonathan Stoddard (J)

Oregon Health & Science University (OHSU), Portland, OR, USA.

Martha Neuringer (M)

Oregon Health & Science University (OHSU), Portland, OR, USA.

David J Granville (DJ)

International Collaboration On Repair Discoveries (ICORD), Vancouver Coastal Health Research Institute, University of British Columbia (UBC), Vancouver, BC, Canada.
Department of Pathology and Laboratory Medicine, UBC, Vancouver, BC, Canada.

Joanne A Matsubara (JA)

Department of Ophthalmology and Visual Sciences, UBC, Vancouver, BC, Canada. jms@mail.ubc.ca.

Classifications MeSH