Anaplastic thyroid cancer spheroids as preclinical models to test therapeutics.
Anaplastic thyroid cancer
Epithelial–mesenchymal transition
Gene expression
Treatment response
Tumor spheroid
Journal
Journal of experimental & clinical cancer research : CR
ISSN: 1756-9966
Titre abrégé: J Exp Clin Cancer Res
Pays: England
ID NLM: 8308647
Informations de publication
Date de publication:
19 Mar 2024
19 Mar 2024
Historique:
received:
11
01
2024
accepted:
10
03
2024
medline:
19
3
2024
pubmed:
19
3
2024
entrez:
19
3
2024
Statut:
epublish
Résumé
Anaplastic thyroid cancer (ATC) is the most aggressive thyroid cancer. Despite advances in tissue culture techniques, a robust model for ATC spheroid culture is yet to be developed. In this study, we created an efficient and cost-effective 3D tumor spheroids culture system from human ATC cells and existing cell lines that better mimic patient tumors and that can enhance our understanding of in vivo treatment response. We found that patient-derived ATC cells and cell lines can readily form spheroids in culture with a unique morphology, size, and cytoskeletal organization. We observed both cohesive (dense and solid structures) and discohesive (irregularly shaped structures) spheroids within the same culture condition across different cell lines. BRAF
Identifiants
pubmed: 38500204
doi: 10.1186/s13046-024-03009-8
pii: 10.1186/s13046-024-03009-8
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
85Subventions
Organisme : Stanford Medicine Harry A. Oberhelman Jr. and Mark L. Welton Endowment
ID : Stanford Medicine Harry A. Oberhelman Jr. and Mark L. Welton Endowment
Informations de copyright
© 2024. The Author(s).
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