Divergent evolution of the alcohol-forming pathway of wax biosynthesis among bryophytes.
Marchantia polymorpha
Physcomitrium patens
bifunctional wax ester synthase/acyl-CoA:diacylglycerol acyltransferase
cuticle
evolution
fatty acyl reductase
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
19 Mar 2024
19 Mar 2024
Historique:
received:
27
11
2023
accepted:
01
03
2024
medline:
19
3
2024
pubmed:
19
3
2024
entrez:
19
3
2024
Statut:
aheadofprint
Résumé
The plant cuticle is a hydrophobic barrier, which seals the epidermal surface of most aboveground organs. While the cuticle biosynthesis of angiosperms has been intensively studied, knowledge about its existence and composition in nonvascular plants is scarce. Here, we identified and characterized homologs of Arabidopsis thaliana fatty acyl-CoA reductase (FAR) ECERIFERUM 4 (AtCER4) and bifunctional wax ester synthase/acyl-CoA:diacylglycerol acyltransferase 1 (AtWSD1) in the liverwort Marchantia polymorpha (MpFAR2 and MpWSD1) and the moss Physcomitrium patens (PpFAR2A, PpFAR2B, and PpWSD1). Although bryophyte harbor similar compound classes as described for angiosperm cuticles, their biosynthesis may not be fully conserved between the bryophytes M. polymorpha and P. patens or between these bryophytes and angiosperms. While PpFAR2A and PpFAR2B contribute to the production of primary alcohols in P. patens, loss of MpFAR2 function does not affect the wax profile of M. polymorpha. By contrast, MpWSD1 acts as the major wax ester-producing enzyme in M. polymorpha, whereas mutations of PpWSD1 do not affect the wax ester levels of P. patens. Our results suggest that the biosynthetic enzymes involved in primary alcohol and wax ester formation in land plants have either evolved multiple times independently or undergone pronounced radiation followed by the formation of lineage-specific toolkits.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Alexander von Humboldt-Stiftung
ID : CAN 1210075 HFST-P
Organisme : Natural Sciences and Engineering Research Council of Canada
Organisme : Deutsche Forschungsgemeinschaft
ID : GRK 2172
Organisme : Deutsche Forschungsgemeinschaft
ID : VR 132/13-1
Organisme : Deutsche Forschungsgemeinschaft
ID : VR 132/4-1
Organisme : National Research Foundation of Korea
ID : NRF-2021R1A2C1006049
Organisme : H2020 European Research Council
ID : 852725
Organisme : H2020 European Research Council
ID : MSCA-IF-EF-ST:892532-SMFP
Informations de copyright
© 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation.
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