A novel laccase from Trametes polyzona with high performance in the decolorization of textile dyes.
Trametes polyzona
Acetosyringone
Laccase
Laccase mediator system
Textile dyes
White-rot fungi
Journal
AMB Express
ISSN: 2191-0855
Titre abrégé: AMB Express
Pays: Germany
ID NLM: 101561785
Informations de publication
Date de publication:
20 Mar 2024
20 Mar 2024
Historique:
received:
26
02
2024
accepted:
04
03
2024
medline:
20
3
2024
pubmed:
20
3
2024
entrez:
20
3
2024
Statut:
epublish
Résumé
Laccases are multicopper oxidases able to oxidize several phenolic compounds and find application in numerous industrial applications. Among laccase producers, white-rot fungi represent a valuable source of multiple isoforms and isoenzymes of these multicopper oxidases. Here we describe the identification, biochemical characterization, and application of laccase 2 from Trametes polyzona (TP-Lac2), a basidiomycete fungus emerged among others that have been screened by plate assay. This enzyme has an optimal temperature of 50 °C and in acidic conditions it is able to oxidize both phenolic and non-phenolic compounds. The ability of TP-Lac2 to decolorize textile dyes was tested in the presence of natural and synthetic mediators at 30 °C and 50 °C. Our results indicate that TP-Lac2 most efficiently decolorizes (decolorization rate > 75%) malachite green oxalate, orange G, amido black10B and bromocresol purple in the presence of acetosyringone and 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate)-ABTS. Overall, the laccase mediator system consisting of TP-Lac2 and the natural mediator acetosyringone has potential as an environmentally friendly alternative for wastewater treatment in the textile industry.
Identifiants
pubmed: 38506984
doi: 10.1186/s13568-024-01687-3
pii: 10.1186/s13568-024-01687-3
doi:
Types de publication
Journal Article
Langues
eng
Pagination
32Subventions
Organisme : Fondazione Cariplo
ID : 2015-0375
Informations de copyright
© 2024. The Author(s).
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