Efficient and economic protein labeling for NMR in mammalian expression systems: Application to a preT-cell and T-cell receptor protein.
19F‐labeling
Expi293F
T‐cell receptor (TCR)
glycosylation
local deuteration
mammalian expression
methyl TROSY
nuclear magnetic resonance spectroscopy (NMR)
preT‐cell receptor (preTCR)
Journal
Protein science : a publication of the Protein Society
ISSN: 1469-896X
Titre abrégé: Protein Sci
Pays: United States
ID NLM: 9211750
Informations de publication
Date de publication:
Apr 2024
Apr 2024
Historique:
revised:
05
02
2024
received:
20
11
2023
accepted:
16
02
2024
medline:
21
3
2024
pubmed:
21
3
2024
entrez:
21
3
2024
Statut:
ppublish
Résumé
Protein nuclear magnetic resonance (NMR) spectroscopy relies on the ability to isotopically label polypeptides, which is achieved through heterologous expression in various host organisms. Most commonly, Escherichia coli is employed by leveraging isotopically substituted ammonium and glucose to uniformly label proteins with
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e4950Subventions
Organisme : NIH HHS
ID : P01AI143565
Pays : United States
Organisme : NIH HHS
ID : P41GM111135
Pays : United States
Informations de copyright
© 2024 The Protein Society.
Références
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