Integration of cell differentiation and initiation of monoterpenoid indole alkaloid metabolism in seed germination of Catharanthus roseus.
Catharanthus roseus
cell differentiation
idioblast
laticifer
metabolic differentiation
monoterpenoid indole alkaloid
seed germination
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
21 Mar 2024
21 Mar 2024
Historique:
received:
01
12
2023
accepted:
22
02
2024
medline:
22
3
2024
pubmed:
22
3
2024
entrez:
21
3
2024
Statut:
aheadofprint
Résumé
In Catharanthus roseus, monoterpenoid indole alkaloids (MIAs) are produced through the cooperation of four cell types, with final products accumulating in specialized cells known as idioblasts and laticifers. To explore the relationship between cellular differentiation and cell type-specific MIA metabolism, we analyzed the expression of MIA biosynthesis in germinating seeds. Embryos from immature and mature seeds were observed via stereomicroscopy, fluorescence microscopy, and electron microscopy. Time-series MIA and iridoid quantification, along with transcriptome analysis, were conducted to determine the initiation of MIA biosynthesis. In addition, the localization of MIAs was examined using alkaloid staining and imaging mass spectrometry (IMS). Laticifers were present in embryos before seed maturation. MIA biosynthesis commenced 12 h after germination. MIAs accumulated in laticifers of embryos following seed germination, and MIA metabolism is induced after germination in a tissue-specific manner. These findings suggest that cellular morphological differentiation precedes metabolic differentiation. Considering the well-known toxicity and defense role of MIAs in matured plants, MIAs may be an important defense strategy already in the delicate developmental phase of seed germination, and biosynthesis and accumulation of MIAs may require the tissue and cellular differentiation.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Society for the Promotion of Science
ID : 16H04807
Organisme : Japan Society for the Promotion of Science
ID : 18H05493
Organisme : Japan Society for the Promotion of Science
ID : 22120006
Organisme : Japan Society for the Promotion of Science
ID : 22K15136
Organisme : Japan Science and Technology Agency
ID : JPMJGX23B0
Organisme : Japan Science and Technology Agency
ID : JPMJSP2125
Informations de copyright
© 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation.
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