A defined diet for pre-adult Drosophila melanogaster.
Acetate
Amino acids
Developmental timing
Fruit flies
Holidic diet
Lipids
Nutrigenomics
Precision nutrition
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
23 Mar 2024
23 Mar 2024
Historique:
received:
17
10
2023
accepted:
20
03
2024
medline:
24
3
2024
pubmed:
24
3
2024
entrez:
24
3
2024
Statut:
epublish
Résumé
Drosophila melanogaster is unique among animal models because it has a fully defined synthetic diet available to study nutrient-gene interactions. However, use of this diet is limited to adult studies due to impaired larval development and survival. Here, we provide an adjusted formula that reduces the developmental period, restores fat levels, enhances body mass, and fully rescues survivorship without compromise to adult lifespan. To demonstrate an application of this formula, we explored pre-adult diet compositions of therapeutic potential in a model of an inherited metabolic disorder affecting the metabolism of branched-chain amino acids. We reveal rapid, specific, and predictable nutrient effects on the disease state consistent with observations from mouse and patient studies. Together, our diet provides a powerful means with which to examine the interplay between diet and metabolism across all life stages in an animal model.
Identifiants
pubmed: 38521863
doi: 10.1038/s41598-024-57681-z
pii: 10.1038/s41598-024-57681-z
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6974Subventions
Organisme : NIH HHS
ID : 5U01HG007530-08
Pays : United States
Informations de copyright
© 2024. The Author(s).
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