The temporal protein signature analyses of developing human deciduous molar tooth germ.
human deciduous molar
label‐free proteome
stem cell differentiation
temporal expression pattern
Journal
Proteomics
ISSN: 1615-9861
Titre abrégé: Proteomics
Pays: Germany
ID NLM: 101092707
Informations de publication
Date de publication:
24 Mar 2024
24 Mar 2024
Historique:
revised:
31
01
2024
received:
19
10
2023
accepted:
05
03
2024
medline:
24
3
2024
pubmed:
24
3
2024
entrez:
24
3
2024
Statut:
aheadofprint
Résumé
The tooth serves as an exemplary model for developmental studies, encompassing epithelial-mesenchymal transition and cell differentiation. The essential factors and pathways identified in tooth development will help understand the natural development process and the malformations of mineralized tissues such as skeleton. The time-dependent proteomic changes were investigated through the proteomics of healthy human molars during embryonic stages, ranging from the cap-to-early bell stage. A comprehensive analysis revealed 713 differentially expressed proteins (DEPs) exhibiting five distinct temporal expression patterns. Through the application of weighted gene co-expression network analysis (WGCNA), 24 potential driver proteins of tooth development were screened, including CHID1, RAP1GDS1, HAPLN3, AKAP12, WLS, GSS, DDAH1, CLSTN1, AFM, RBP1, AGO1, SET, HMGB2, HMGB1, ANP32A, SPON1, FREM1, C8B, PRPS2, FCHO2, PPP1R12A, GPALPP1, U2AF2, and RCC2. Then, the proteomics and transcriptomics expression patterns of these proteins were further compared, complemented by single-cell RNA-sequencing (scRNA-seq). In summary, this study not only offers a wealth of information regarding the molecular intricacies of human embryonic epithelial and mesenchymal cell differentiation but also serves as an invaluable resource for future mechanistic inquiries into tooth development.
Identifiants
pubmed: 38522031
doi: 10.1002/pmic.202300396
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2300396Subventions
Organisme : Foundation of Longgang District Key Laboratory of Birth Defects Prevention
ID : No. LGKCZSYS2018000010
Organisme : Economic and Technological Development Special Foundation of Shenzhen Longgang District
ID : No. LGKCYLWS2021000026
Informations de copyright
© 2024 Wiley‐VCH GmbH.
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