Development and Validation of Micro-Azocasein Assay for Quantifying Bromelain.

N-acetylcysteine azocasein bromelain enzymes proteolytic activity validation

Journal

Methods and protocols
ISSN: 2409-9279
Titre abrégé: Methods Protoc
Pays: Switzerland
ID NLM: 101720073

Informations de publication

Date de publication:
15 Mar 2024
Historique:
received: 17 01 2024
revised: 08 03 2024
accepted: 08 03 2024
medline: 25 3 2024
pubmed: 25 3 2024
entrez: 25 3 2024
Statut: epublish

Résumé

The proteolytic activity of enzymes may be evaluated by a colorimetric method with azocasein. Hence, we developed a micro-assay to quantify bromelain using azocasein. A total of 250 µL of 1.0% azocasein in dH

Identifiants

DOI: 10.3390/mps7020025 PMID: 38525783
pubmed: 38525783
pii: mps7020025
doi: 10.3390/mps7020025
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : Mucpharm Pty Ltd
ID : N/A

Auteurs

Krishna Pillai (K)

Mucpharm Pty Ltd., Sydney, NSW 2217, Australia.
Department of Surgery, St. George Hospital, Sydney, NSW 2217, Australia.

Javed Akhter (J)

Mucpharm Pty Ltd., Sydney, NSW 2217, Australia.
Department of Surgery, St. George Hospital, Sydney, NSW 2217, Australia.

Ahmed H Mekkawy (AH)

Mucpharm Pty Ltd., Sydney, NSW 2217, Australia.
Department of Surgery, St. George Hospital, Sydney, NSW 2217, Australia.
St. George & Sutherland Clinical School, University of New South Wales, Sydney, NSW 2217, Australia.
ORCID: 0000-0001-7162-8936

Sarah J Valle (SJ)

Mucpharm Pty Ltd., Sydney, NSW 2217, Australia.
Department of Surgery, St. George Hospital, Sydney, NSW 2217, Australia.
Intensive Care Unit, St. George Hospital, Sydney, NSW 2217, Australia.

David L Morris (DL)

Mucpharm Pty Ltd., Sydney, NSW 2217, Australia.
Department of Surgery, St. George Hospital, Sydney, NSW 2217, Australia.
St. George & Sutherland Clinical School, University of New South Wales, Sydney, NSW 2217, Australia.

Classifications MeSH