Preliminary report of Mycoplasma Wenoynii and Candidatus Mycoplasma haemobos infection in Korean native cattle.

Candidatus Mycoplasma haemobos Mycoplasma wenyonii Grazing Korean native cattle anemia

Journal

BMC veterinary research
ISSN: 1746-6148
Titre abrégé: BMC Vet Res
Pays: England
ID NLM: 101249759

Informations de publication

Date de publication:
26 Mar 2024
Historique:
received: 11 08 2023
accepted: 14 03 2024
medline: 27 3 2024
pubmed: 27 3 2024
entrez: 27 3 2024
Statut: epublish

Résumé

Hemotropic mycoplasmas or hemoplasmas are bacteria that attach to the erythrocyte surface and cause bovine hemoplasmosis. Two species, Mycoplasma wenyonii and Candidatus Mycoplasma haemobos, have been identified and shown to be distributed worldwide. However, there is currently no information available on hemoplasmas in cattle in the Republic of Korea. The aim of this study was to investigate the presence of hemoplasmas in Korean native cattle and to evaluate the association between hemoplasma infection and anemia. One farm was selected, at which blood samples were collected from 104 Korean native cattle [grazing cattle (n = 89) and housed cattle (n = 15)]. Hemoplasmas were detected via polymerase chain reaction analysis and complete blood counts were also performed. The overall prevalence of hemoplasmas was 34% (35/104); 20.2% (21/104) for M. wenyonii, 3.8% (4/104) for C. M. haemobos, and 9.6% (10/104) for co-infection. Candidatus Mycoplasma haemobos was detected only in grazing cattle. Of red blood cell (RBC) parameters, C. M. haemobos-infected cattle had lower RBC and hematocrit, and higher mean cell volume than hemoplasma-negative cattle, although none of these differences were statistically significant. This is the first study to report the occurrence of M. wenyonii and C. M. haemobos. Mycoplasma wenyonii is more prevalent than C. M. haemobos in Korean native cattle. The results did not show an association between hemoplasma infection and anemia. Considering the infection rate of hemoplasmas shown in this study, further studies, such as on the pathogenicity and clinical significance of hemoplasmas are necessary.

Sections du résumé

BACKGROUND BACKGROUND
Hemotropic mycoplasmas or hemoplasmas are bacteria that attach to the erythrocyte surface and cause bovine hemoplasmosis. Two species, Mycoplasma wenyonii and Candidatus Mycoplasma haemobos, have been identified and shown to be distributed worldwide. However, there is currently no information available on hemoplasmas in cattle in the Republic of Korea. The aim of this study was to investigate the presence of hemoplasmas in Korean native cattle and to evaluate the association between hemoplasma infection and anemia.
METHODS METHODS
One farm was selected, at which blood samples were collected from 104 Korean native cattle [grazing cattle (n = 89) and housed cattle (n = 15)]. Hemoplasmas were detected via polymerase chain reaction analysis and complete blood counts were also performed.
RESULTS RESULTS
The overall prevalence of hemoplasmas was 34% (35/104); 20.2% (21/104) for M. wenyonii, 3.8% (4/104) for C. M. haemobos, and 9.6% (10/104) for co-infection. Candidatus Mycoplasma haemobos was detected only in grazing cattle. Of red blood cell (RBC) parameters, C. M. haemobos-infected cattle had lower RBC and hematocrit, and higher mean cell volume than hemoplasma-negative cattle, although none of these differences were statistically significant. This is the first study to report the occurrence of M. wenyonii and C. M. haemobos. Mycoplasma wenyonii is more prevalent than C. M. haemobos in Korean native cattle. The results did not show an association between hemoplasma infection and anemia.
CONCLUSIONS CONCLUSIONS
Considering the infection rate of hemoplasmas shown in this study, further studies, such as on the pathogenicity and clinical significance of hemoplasmas are necessary.

Identifiants

DOI: 10.1186/s12917-024-03976-2 PMID: 38532391
pubmed: 38532391
doi: 10.1186/s12917-024-03976-2
pii: 10.1186/s12917-024-03976-2
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

121

Subventions

Organisme : Ministry of Science and ICT, South Korea
ID : 2023-DD-UP-0031
Organisme : National Research Foundation of Korea
ID : NRF-2021R1A2C1011579

Informations de copyright

© 2024. The Author(s).

Références

Chae JS, Adjemian JZ, Kim HC, Ko S, Klein TA, Foley J. Predicting the emergence of tick-borne infections based on climatic changes in Korea. Vector Borne Zoonotic Dis. 2008;8:265–75. https://doi.org/10.1089/vbz.2007.0190 .
doi: 10.1089/vbz.2007.0190 pubmed: 18429692
Githeko AK, Lindsay SW, Confalonieri UE, Patz JA. Climate change and vector-borne diseases: a regional analysis. Bull World Health Organ. 2000;78:1136–47.
pubmed: 11019462 pmcid: 2560843
de Castro JJ. Sustainable tick and tickborne disease control in livestock improvement in developing countries. Vet Parasitol. 1997;71:77–97. https://doi.org/10.1016/s0304-4017(97)00033-2 .
doi: 10.1016/s0304-4017(97)00033-2 pubmed: 9261972
Fujihara Y, Sasaoka F, Suzuki J, Watanabe Y, Fujihara M, Ooshita K, et al. Prevalence of hemoplasma infection among cattle in the western part of Japan. J Vet Med Sci. 2011;73:1653–5. https://doi.org/10.1292/jvms.11-0269 .
doi: 10.1292/jvms.11-0269 pubmed: 21799295
Maggi RG, Compton SM, Trull CL, Mascarelli PE, Mozayeni BR, Breitschwerdt EB. Infection with hemotropic Mycoplasma species in patients with or without extensive arthropod or animal contact. J Clin Microbiol. 2013;51:3237–41. https://doi.org/10.1128/JCM.01125-13 .
doi: 10.1128/JCM.01125-13 pubmed: 23863574 pmcid: 3811635
Santos NJR, Brito DRB, Abate HL, Paixao SF, Soares EDS, Vieira T et al. Hemotropic mycoplasmas infection in water buffaloes (Bubalus bubalis) from northeastern Brazil. Comp Immunol Microbiol Infect Dis. 2018;56:27 – 9. https://doi.org/0.1016/j.cimid.2017.12.003.
Montes AJ, Wolfe DF, Welles EG, Tyler JW, Tepe E. Infertility associated with Eperythrozoon wenyonii infection in a bull. J Am Vet Med Assoc. 1994;204:261–3.
doi: 10.2460/javma.1994.204.02.261 pubmed: 8144388
Genova SG, Streeter RN, Velguth KE, Snider TA, Kocan KM, Simpson KM. Severe anemia associated with Mycoplasma wenyonii infection in a mature cow. Can Vet J. 2011;52:1018–21.
pubmed: 22379205 pmcid: 3157061
McFadden A, Ha HJ, Donald JJ, Bueno IM, van Andel M, Thompson JC, et al. Investigation of bovine haemoplasmas and their association with anaemia in New Zealand cattle. N Z Vet J. 2016;64:65–8. https://doi.org/10.1080/00480169.2015.1090356 .
doi: 10.1080/00480169.2015.1090356 pubmed: 26411673
Tagawa M, Yamakawa K, Aoki T, Matsumoto K, Ishii M, Inokuma H. Effect of chronic hemoplasma infection on cattle productivity. J Vet Med Sci. 2013;75:1271–5. https://doi.org/10.1292/jvms.13-0119 .
doi: 10.1292/jvms.13-0119 pubmed: 23676278 pmcid: 3942926
Ybanez AP, Ybanez RHD, Armonia RKM, Chico JKE, Ferraren KJV, Tapdasan EP, et al. First molecular detection of Mycoplasma wenyonii and the ectoparasite biodiversity in dairy water buffalo and cattle in Bohol, Philippines. Parasitol Int. 2019;70:77–81. https://doi.org/10.1016/j.parint.2019.02.004 .
doi: 10.1016/j.parint.2019.02.004 pubmed: 30776450
Adler S, Ellenbogen V. A note on two new blood parasites of cattle, Eperythrozoon and Bartonella. J Comp Pathol Th. 1934;47:219–21. https://doi.org/10.1016/S0368-1742(34)80027-6 .
doi: 10.1016/S0368-1742(34)80027-6
Hofmann-Lehmann R, Meli ML, Dreher UM, Gonczi E, Deplazes P, Braun U, et al. Concurrent infections with vector-borne pathogens associated with fatal hemolytic anemia in a cattle herd in Switzerland. J Clin Microbiol. 2004;42:3775–80. https://doi.org/10.1128/JCM.42.8.3775-3780.2004 .
doi: 10.1128/JCM.42.8.3775-3780.2004 pubmed: 15297529 pmcid: 497630
Tagawa M, Matsumoto K, Inokuma H. Molecular detection of Mycoplasma wenyonii and ‘Candidatus Mycoplasma haemobos’ in cattle in Hokkaido. Japan Vet Microbiol. 2008;132:177–80. https://doi.org/10.1016/j.vetmic.2008.05.006 .
Smith JA, Thrall MA, Smith JL, Salman MD, Ching SV, Collins JK. Eperythrozoon wenyonii infection in dairy cattle. J Am Vet Med Assoc. 1990;196:1244–50.
doi: 10.2460/javma.1990.196.08.1244 pubmed: 2332369
Hoelzle K, Winkler M, Kramer MM, Wittenbrink MM, Dieckmann SM, Hoelzle LE. Detection of Candidatus Mycoplasma haemobos in cattle with anaemia. Vet J. 2011;187:408–10. https://doi.org/10.1016/j.tvjl.2010.01.016 .
doi: 10.1016/j.tvjl.2010.01.016 pubmed: 20188610
Tagawa M, Matsumoto K, Yokoyama N, Inokuma H. Comparison of the effect of two hemoplasma species on hematological parameters in cattle. J Vet Med Sci. 2010;72:113–5. https://doi.org/10.1292/jvms.09-0304 .
doi: 10.1292/jvms.09-0304 pubmed: 19915333
Niethammer FM, Ade J, Hoelzle LE, Schade B. Hemotrophic mycoplasma in Simmental cattle in Bavaria: prevalence, blood parameters, and transplacental transmission of ‘Candidatus Mycoplasma haemobos’ and Mycoplasma wenyonii. Acta Vet Scand. 2018;60:74. https://doi.org/10.1186/s13028-018-0428-y .
doi: 10.1186/s13028-018-0428-y pubmed: 30445976 pmcid: 6240245
Hornok S, Meli ML, Perreten A, Farkas R, Willi B, Beugnet F, et al. Molecular investigation of hard ticks (Acari: Ixodidae) and fleas (Siphonaptera: Pulicidae) as potential vectors of rickettsial and mycoplasmal agents. Vet Microbiol. 2010;140:98–104. https://doi.org/10.1016/j.vetmic.2009.07.013 .
doi: 10.1016/j.vetmic.2009.07.013 pubmed: 19660880
Song Q, Wang L, Fang R, Khan MK, Zhou Y, Zhao J. Detection of Mycoplasma wenyonii in cattle and transmission vectors by the loop-mediated isothermal amplification (LAMP) assay. Trop Anim Health Prod. 2013;45:247–50. https://doi.org/10.1007/s11250-012-0197-y .
doi: 10.1007/s11250-012-0197-y pubmed: 22684637
Diaz-Sanchez AA, Corona-Gonzalez B, Meli ML, Alvarez DO, Canizares EV, Rodriguez OF, et al. First molecular evidence of bovine hemoplasma species (Mycoplasma spp.) in water buffalo and dairy cattle herds in Cuba. Parasit Vectors. 2019;12:78. https://doi.org/10.1186/s13071-019-3325-y .
doi: 10.1186/s13071-019-3325-y pubmed: 30732656 pmcid: 6367761
Hornok S, Micsutka A, Meli ML, Lutz H, Hofmann-Lehmann R. Molecular investigation of transplacental and vector-borne transmission of bovine haemoplasmas. Vet Microbiol. 2011;152:411–4. https://doi.org/10.1016/j.vetmic.2011.04.031 .
doi: 10.1016/j.vetmic.2011.04.031 pubmed: 21605950
Girotto-Soares A, Soares JF, Bogado ALG, de Macedo CAB, Sandeski LM, Garcia JL, et al. Candidatus Mycoplasma haemobos’: transplacental transmission in dairy cows (Bos taurus). Vet Microbiol. 2016;195:22–4. https://doi.org/10.1016/j.vetmic.2016.08.020 .
de Mello VVC, de Souza Ramos IA, Herrera HM, Mendes NS, Calchi AC, Campos JBV, et al. Occurrence and genetic diversity of hemoplasmas in beef cattle from the Brazilian pantanal, an endemic area for bovine trypanosomiasis in South America. Comp Immunol Microbiol Infect Dis. 2019;66:101337. https://doi.org/10.1016/j.cimid.2019.101337 .
doi: 10.1016/j.cimid.2019.101337 pubmed: 31437678
Schambow RA, Poulsen K, Bolin S, Krahn D, Norby B, Sockett D, et al. Apparent prevalence of Mycoplasma wenyonii, Candidatus Mycoplasma haemobos, and bovine leukemia virus in Wisconsin and Michigan dairy cattle herds. JDS Commun. 2021;2:61–6. https://doi.org/10.3168/jdsc.2020-0033 .
doi: 10.3168/jdsc.2020-0033 pubmed: 36338775 pmcid: 9623634
Tatsukawa F, Nohara R, Taniguchi T, Goto A, Misawa N, Katamoto H. Detection of Mycoplasma wenyonii and Candidatus Mycoplasma haemobos from Japanese black breeding cows in Kyushu and Okinawa region, southern part of Japan. J Vet Med Sci. 2021;83:9–16. https://doi.org/10.1292/jvms.20-0505 .
doi: 10.1292/jvms.20-0505 pubmed: 33162434
Deeney AS, Collins R, Ridley AM. Identification of Mycoplasma species and related organisms from ruminants in England and Wales during 2005–2019. BMC Vet Res. 2021;17:325. https://doi.org/10.1186/s12917-021-03037-y .
doi: 10.1186/s12917-021-03037-y pubmed: 34641885 pmcid: 8513359
Ferrari LDR, Hassan-Kadle AA, Collere FCM, Coradi VS, Ibrahim AM, Osman AM, et al. Hemoplasmas and ticks in cattle from Somalia. Acta Trop. 2022;236:106696. https://doi.org/10.1016/j.actatropica.2022.106696 .
doi: 10.1016/j.actatropica.2022.106696 pubmed: 36189489
Erol U, Sahin OF, Altay K. Molecular prevalence of bovine hemoplasmosis in Turkey with first detection of Mycoplasma wenyonii and Candidatus Mycoplasma haemobos in cattle and water buffalo. Vet Res Commun. 2023;47:207–15. https://doi.org/10.1007/s11259-022-09943-2 .
doi: 10.1007/s11259-022-09943-2 pubmed: 35624402
Altay K, Sahin OF, Erol U, Aytmirzakizi A. First molecular detection and phylogenetic analysis of Mycoplasma wenyonii and Candidatus Mycoplasma haemobos in cattle in different parts of Kyrgyzstan. Biologia. 2023;78:633–40.
doi: 10.1007/s11756-022-01292-4
Byamukama B, Tumwebaze MA, Tayebwa DS, Byaruhanga J, Angwe MK, Li J, et al. First molecular detection and characterization of hemotropic Mycoplasma species in cattle and goats from Uganda. Anim (Basel). 2020; 10:1624. https://doi.org/10.3390/ani10091624 .
Happi AN, Osifade O, Oluniyi PE, Ogunro BN. Comparison of light microscopy and polymerase chain reaction for the detection of haemoparasites in cattle in Nigeria. Acta Parasitol. 2020;65:44–56. https://doi.org/0.2478/s11686-019-00123-y.
Meli ML, Willi B, Dreher UM, Cattori V, Knubben-Schweizer G, Nuss K, et al. Identification, molecular characterization, and occurrence of two bovine hemoplasma species in Swiss cattle and development of real-time TaqMan quantitative PCR assays for diagnosis of bovine hemoplasma infections. J Clin Microbiol. 2010;48:3563–8. https://doi.org/10.1128/JCM.02224-09 .
doi: 10.1128/JCM.02224-09 pubmed: 20686093 pmcid: 2953077
Su QL, Song HQ, Lin RQ, Yuan ZG, Yang JF, Zhao GH, et al. The detection of Candidatus Mycoplasma haemobos in cattle and buffalo in China. Trop Anim Health Prod. 2010;42:1805–8. https://doi.org/10.1007/s11250-010-9640-0 .
doi: 10.1007/s11250-010-9640-0 pubmed: 20596775
Girotto A, Zangirolamo AF, Bogado AL, Souza AS, da Silva GC, Garcia JL, et al. Molecular detection and occurrence of ‘Candidatus Mycoplasma haemobos’ in dairy cattle of Southern Brazil. Rev Bras Parasitol Vet. 2012;21:342–4. https://doi.org/10.1590/s1984-29612012000300034 .
doi: 10.1590/s1984-29612012000300034 pubmed: 23070456
Tagawa M, Ybanez AP, Matsumoto K, Yokoyama N, Inokuma H. Prevalence and risk factor analysis of bovine hemoplasma infection by direct PCR in Eastern Hokkaido, Japan. J Vet Med Sci. 2012;74:1171–6. https://doi.org/10.1292/jvms.12-0118 .
doi: 10.1292/jvms.12-0118 pubmed: 22673725
Kamani J, Shand M, Shekaro A, Laminu B, Toyin O, Abasiama MS, et al. Mycoplasma wenyonii and Candidatus Mycoplasma haemobos in pastoralists cattle in Nigeria. Acta Parasitol. 2023;68:430–8. https://doi.org/10.1007/s11686-023-00683-0 .
doi: 10.1007/s11686-023-00683-0 pubmed: 37170044
Mohd Hasan LI, Kho KL, Koh FX, Hassan Nizam QN, Tay ST. Molecular evidence of hemoplasmas in Malaysian cattle and ticks. Trop Biomed. 2017;34:668–74.
pubmed: 33592935
Shi H, Duan L, Liu F, Hu Y, Shi Z, Chen X, et al. Rhipicephalus (Boophilus) microplus ticks as reservoir and vector of ‘Candidatus Mycoplasma haemobos’ in China. Vet Parasitol. 2019;274:108929. https://doi.org/10.1016/j.vetpar.2019.108929 .
doi: 10.1016/j.vetpar.2019.108929 pubmed: 31568995
Thongmeesee K, Chonglomkrod B, Srisakdi C, Saributr M, Suksai P, Kamkong P, et al. Molecular detection of Mycoplasma wenyonii and its closely related hemotropic Mycoplasmasp. in blood-sucking flies from a buffalo farm in Chachoengsao Province, Thailand. Acta Trop. 2022;235:106647. https://doi.org/10.1016/j.actatropica.2022.106647 .

Auteurs

Youngjun Kim (Y)

Department of Animal Hospital, Genetic Improvement Center, National Agricultural Cooperative Federation, Hanwoo, Seosan, 31948, Republic of Korea.
Department of Veterinary Internal Medicine, College of Veterinary Medicine, Jeonbuk University, Iksan, 54596, Republic of Korea.

Hannah Kim (H)

College of Arts and Sciences, University of Washington, Seattle, WA, 98195, USA.

Jae-Hyeon Choi (JH)

Department of Horse/Companion and Wild Animals, College of Ecology and Environmental Science, Kyungpook National University, Sangju, 37224, Republic of Korea.

Hyung-Chul Cho (HC)

Department of Animal Science and Biotechnology, College of Ecology and Environmental Science, Kyungpook National University, Sangju, 37224, Republic of Korea.

Min-Jeong Ji (MJ)

Department of Animal Science and Biotechnology, College of Ecology and Environmental Science, Kyungpook National University, Sangju, 37224, Republic of Korea.

Yu-Jin Park (YJ)

Department of Animal Science and Biotechnology, College of Ecology and Environmental Science, Kyungpook National University, Sangju, 37224, Republic of Korea.

Jinho Park (J)

Department of Veterinary Internal Medicine, College of Veterinary Medicine, Jeonbuk University, Iksan, 54596, Republic of Korea. jpark@jbnu.ac.kr.

Kyoung-Seong Choi (KS)

Department of Horse/Companion and Wild Animals, College of Ecology and Environmental Science, Kyungpook National University, Sangju, 37224, Republic of Korea. kschoi3@knu.ac.kr.
Department of Animal Science and Biotechnology, College of Ecology and Environmental Science, Kyungpook National University, Sangju, 37224, Republic of Korea. kschoi3@knu.ac.kr.

Classifications MeSH