Analysis of the effect of CCR7 on the microenvironment of mouse oral squamous cell carcinoma by single-cell RNA sequencing technology.
CCR7
OSCC
TME
scRNA-seq
Journal
Journal of experimental & clinical cancer research : CR
ISSN: 1756-9966
Titre abrégé: J Exp Clin Cancer Res
Pays: England
ID NLM: 8308647
Informations de publication
Date de publication:
27 Mar 2024
27 Mar 2024
Historique:
received:
27
11
2023
accepted:
15
03
2024
medline:
28
3
2024
pubmed:
28
3
2024
entrez:
28
3
2024
Statut:
epublish
Résumé
Studies have shown that CCR7, an important inflammatory factor, can promote the proliferation and metastasis of oral squamous cell carcinoma (OSCC), but its role in the tumor microenvironment (TME) remains unclear. This paper explores the role of CCR7 in the TME of OSCC. In this work, we constructed CCR7 gene knockout mice and OSCC mouse models. Single-cell RNA sequencing (scRNA-seq) and bioinformatics were used to analyze the differences in the OSCC microenvironment between three CCR7 gene knockout mice (KO) and three wild-type mice (WT). Immunohistochemistry, immunofluorescence staining, and flow cytometry were used to analyze the expression of key genes in significantly different cell types between the KO and WT groups. An in vitro experiment was used to verify the effect of CCR7 on M2 macrophage polarization. In the mouse OSCC models, the tumor growth rate in the KO group was significantly lower than that in the WT group. Eight main cell types (including tumor cells, fibroblasts, macrophages, granulocytes, T cells, endothelial cells, monocytes, and B cells) were identified by Seurat analysis. The scRNA-seq results showed that the proportion of tumor cells was lower, but the proportion of inflammatory cells was significantly higher in the KO group than in the WT group. CellPhoneDB analysis results indicated a strong interaction relationship between tumor cells and macrophages, T cells, fibroblasts, and endothelial cells. Functional enrichment results indicated that the expression level of the Dusp1 gene in the KO group was generally higher than that in the WT group in various cell types. Macrophage subclustering results indicated that the proportion of M2 macrophages in the KO group was lower than that in the WT group. In vitro experimental results showed that CCR7 can promote M2 macrophage polarization, thus promoting the proliferation, invasion and migration of OSCC cells. CCR7 gene knockout can significantly inhibit the growth of mouse oral squamous cell carcinoma by promoting the polarization of M2 macrophages.
Sections du résumé
BACKGROUND
BACKGROUND
Studies have shown that CCR7, an important inflammatory factor, can promote the proliferation and metastasis of oral squamous cell carcinoma (OSCC), but its role in the tumor microenvironment (TME) remains unclear. This paper explores the role of CCR7 in the TME of OSCC.
METHODS
METHODS
In this work, we constructed CCR7 gene knockout mice and OSCC mouse models. Single-cell RNA sequencing (scRNA-seq) and bioinformatics were used to analyze the differences in the OSCC microenvironment between three CCR7 gene knockout mice (KO) and three wild-type mice (WT). Immunohistochemistry, immunofluorescence staining, and flow cytometry were used to analyze the expression of key genes in significantly different cell types between the KO and WT groups. An in vitro experiment was used to verify the effect of CCR7 on M2 macrophage polarization.
RESULTS
RESULTS
In the mouse OSCC models, the tumor growth rate in the KO group was significantly lower than that in the WT group. Eight main cell types (including tumor cells, fibroblasts, macrophages, granulocytes, T cells, endothelial cells, monocytes, and B cells) were identified by Seurat analysis. The scRNA-seq results showed that the proportion of tumor cells was lower, but the proportion of inflammatory cells was significantly higher in the KO group than in the WT group. CellPhoneDB analysis results indicated a strong interaction relationship between tumor cells and macrophages, T cells, fibroblasts, and endothelial cells. Functional enrichment results indicated that the expression level of the Dusp1 gene in the KO group was generally higher than that in the WT group in various cell types. Macrophage subclustering results indicated that the proportion of M2 macrophages in the KO group was lower than that in the WT group. In vitro experimental results showed that CCR7 can promote M2 macrophage polarization, thus promoting the proliferation, invasion and migration of OSCC cells.
CONCLUSIONS
CONCLUSIONS
CCR7 gene knockout can significantly inhibit the growth of mouse oral squamous cell carcinoma by promoting the polarization of M2 macrophages.
Identifiants
pubmed: 38539232
doi: 10.1186/s13046-024-03013-y
pii: 10.1186/s13046-024-03013-y
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
94Subventions
Organisme : Foundation of Liaoning Province Education Administration
ID : JC2019025
Organisme : Liaoning Science and Technology Program
ID : 2019-ZD-0751
Organisme : Special Funds of "First-Class Universities and Disciplines of the World" Project
ID : 115-3110210730
Organisme : National Natural Science Foundation of China
ID : 82203680
Organisme : Natural Science Foundation of Liaoning Province
ID : 2021-MS-176
Informations de copyright
© 2024. The Author(s).
Références
Chamoli A, Gosavi AS, Shirwadkar UP, Wangdale KV, Behera SK, Kurrey NK, Kalia K, Mandoli A. Overview of oral cavity squamous cell carcinoma: risk factors, mechanisms, and diagnostics. Oral Oncol. 2021;121:105451.
pubmed: 34329869
doi: 10.1016/j.oraloncology.2021.105451
Warnakulasuriya S, Kujan O, Aguirre-Urizar JM, Bagan JV, González-Moles M, Kerr AR, Lodi G, Mello FW, Monteiro L, Ogden GR, et al. Oral potentially malignant disorders: a consensus report from an international seminar on nomenclature and classification, convened by the WHO collaborating centre for oral cancer. Oral Dis. 2021;27:1862–80.
pubmed: 33128420
doi: 10.1111/odi.13704
Machiels J, René Leemans C, Golusinski W, Grau C, Licitra L, Gregoire V. JAooojotESfMO: Squamous cell carcinoma of the oral cavity, larynx, oropharynx and hypopharynx: EHNS-ESMO-ESTRO clinical practice guidelines for diagnosis, treatment and follow-up. Ann Oncol. 2020;31:1462–75.
pubmed: 33239190
doi: 10.1016/j.annonc.2020.07.011
Kwapisz D. Pembrolizumab and atezolizumab in triple-negative breast cancer. Cancer Immunol Immunother. 2021;70:607–17.
pubmed: 33015734
doi: 10.1007/s00262-020-02736-z
Alsahafi E, Begg K, Amelio I, Raulf N, Lucarelli P, Sauter T, Tavassoli M. Clinical update on head and neck cancer: molecular biology and ongoing challenges. Cell Death Dis. 2019;10:540.
pubmed: 31308358
pmcid: 6629629
doi: 10.1038/s41419-019-1769-9
Ferris RL, Blumenschein G Jr, Fayette J, Guigay J, Colevas AD, Licitra L, Harrington K, Kasper S, Vokes EE, Even C, et al. Nivolumab for recurrent squamous-cell carcinoma of the head and neck. N Engl J Med. 2016;375:1856–67.
pubmed: 27718784
pmcid: 5564292
doi: 10.1056/NEJMoa1602252
Maman S, Witz IP. A history of exploring cancer in context. Nat Rev Cancer. 2018;18:359–76.
pubmed: 29700396
doi: 10.1038/s41568-018-0006-7
Elmusrati AA, Pilborough AE, Khurram SA, Lambert DW. Cancer-associated fibroblasts promote bone invasion in oral squamous cell carcinoma. Br J Cancer. 2017;117:867–75.
pubmed: 28742795
pmcid: 5589989
doi: 10.1038/bjc.2017.239
Essa AA, Yamazaki M, Maruyama S, Abé T, Babkair H, Raghib AM, Megahed EM, Cheng J, Saku T. Tumour-associated macrophages are recruited and differentiated in the neoplastic stroma of oral squamous cell carcinoma. Pathology. 2016;48:219–27.
pubmed: 27020496
doi: 10.1016/j.pathol.2016.02.006
Peltanova B, Raudenska M, Masarik M. Effect of tumor microenvironment on pathogenesis of the head and neck squamous cell carcinoma: a systematic review. Mol Cancer. 2019;18:63.
pubmed: 30927923
pmcid: 6441173
doi: 10.1186/s12943-019-0983-5
Wang S, Sun M, Gu C, Wang X, Chen D, Zhao E, Jiao X, Zheng J. Expression of CD163, interleukin-10, and interferon-gamma in oral squamous cell carcinoma: mutual relationships and prognostic implications. Eur J Oral Sci. 2014;122:202–9.
pubmed: 24796206
doi: 10.1111/eos.12131
Xiao M, Zhang J, Chen W, Chen W. M1-like tumor-associated macrophages activated by exosome-transferred THBS1 promote malignant migration in oral squamous cell carcinoma. J Exp Clin Cancer Res. 2018;37:143.
pubmed: 29986759
pmcid: 6038304
doi: 10.1186/s13046-018-0815-2
Mori K, Hiroi M, Shimada J, Ohmori Y. Infiltration of m2 tumor-associated macrophages in oral squamous cell carcinoma correlates with tumor malignancy. Cancers (Basel). 2011;3:3726–39.
pubmed: 24213108
doi: 10.3390/cancers3043726
Costa NL, Valadares MC, Souza PP, Mendonça EF, Oliveira JC, Silva TA, Batista AC. Tumor-associated macrophages and the profile of inflammatory cytokines in oral squamous cell carcinoma. Oral Oncol. 2013;49:216–23.
pubmed: 23089461
doi: 10.1016/j.oraloncology.2012.09.012
Nguyen N, Bellile E, Thomas D, McHugh J, Rozek L, Virani S, Peterson L, Carey TE, Walline H, Moyer J, et al. Tumor infiltrating lymphocytes and survival in patients with head and neck squamous cell carcinoma. Head Neck. 2016;38:1074–84.
pubmed: 26879675
pmcid: 4900934
doi: 10.1002/hed.24406
de Ruiter EJ, Ooft ML, Devriese LA, Willems SM. The prognostic role of tumor infiltrating T-lymphocytes in squamous cell carcinoma of the head and neck: a systematic review and meta-analysis. Oncoimmunology. 2017;6:e1356148.
pubmed: 29147608
pmcid: 5674970
doi: 10.1080/2162402X.2017.1356148
Quail DF, Joyce JA. Microenvironmental regulation of tumor progression and metastasis. Nat Med. 2013;19:1423–37.
pubmed: 24202395
pmcid: 3954707
doi: 10.1038/nm.3394
Förster R, Davalos-Misslitz AC, Rot A. CCR7 and its ligands: balancing immunity and tolerance. Nat Rev Immunol. 2008;8:362–71.
pubmed: 18379575
doi: 10.1038/nri2297
Zhang Z, Liu F, Li Z, Wang D, Li R. Sun CJOl: Jak3 is involved in CCR7-dependent migration and invasion in metastatic squamous cell carcinoma of the head and neck. Oncol Lett. 2017;13:3191–7.
pubmed: 28521425
pmcid: 5431255
doi: 10.3892/ol.2017.5861
Liu F, Zhao Z, Li P, Ding X, Zong Z. Sun CJTBjoo, surgery m: Mammalian target of rapamycin (mTOR) is involved in the survival of cells mediated by chemokine receptor 7 through PI3K/Akt in metastatic squamous cell carcinoma of the head and neck. Br J Oral Maxillofac Surg. 2010;48:291–6.
pubmed: 19615795
doi: 10.1016/j.bjoms.2009.06.007
Zhen-jin Z, Peng L, Fa-yu L, Liyan S, Chang-fu SJM. biochemistry c: PKCα take part in CCR7/NF-κB autocrine signaling loop in CCR7-positive squamous cell carcinoma of head and neck. Mol Cell Biochem. 2011;357:181–7.
pubmed: 21735098
doi: 10.1007/s11010-011-0888-0
Zhao Z, Liu F, Li P, Ding X, Zong Z. Sun CJOr: CCL19-induced chemokine receptor 7 activates the phosphoinositide-3 kinase-mediated invasive pathway through Cdc42 in metastatic squamous cell carcinoma of the head and neck. Oncol Rep. 2011;25:729–37.
pubmed: 21165582
Boyle ST, Ingman WV, Poltavets V, Faulkner JW, Whitfield RJ, McColl SR, Kochetkova M. The chemokine receptor CCR7 promotes mammary tumorigenesis through amplification of stem-like cells. Oncogene. 2016;35:105–15.
pubmed: 25772241
doi: 10.1038/onc.2015.66
Judd NP, Allen CT, Winkler AE, Uppaluri R. Comparative analysis of tumor-infiltrating lymphocytes in a syngeneic mouse model of oral cancer. Otolaryngol Head Neck Surg. 2012;147:493–500.
pubmed: 22434099
pmcid: 6346425
doi: 10.1177/0194599812442037
Chanput W, Mes JJ, Wichers HJ. THP-1 cell line: an in vitro cell model for immune modulation approach. Int Immunopharmacol. 2014;23:37–45.
pubmed: 25130606
doi: 10.1016/j.intimp.2014.08.002
Daigneault M, Preston JA, Marriott HM, Whyte MK, Dockrell DH. The identification of markers of macrophage differentiation in PMA-stimulated THP-1 cells and monocyte-derived macrophages. PLoS One. 2010;5:e8668.
pubmed: 20084270
pmcid: 2800192
doi: 10.1371/journal.pone.0008668
Judd NP, Winkler AE, Murillo-Sauca O, Brotman JJ, Law JH, Lewis JS Jr, Dunn GP, Bui JD, Sunwoo JB, Uppaluri R. ERK1/2 regulation of CD44 modulates oral cancer aggressiveness. Cancer Res. 2012;72:365–74.
pubmed: 22086849
doi: 10.1158/0008-5472.CAN-11-1831
Liberzon A. A description of the Molecular Signatures Database (MSigDB) Web site. Methods Mol Biol. 2014;1150:153–60.
pubmed: 24743996
doi: 10.1007/978-1-4939-0512-6_9
Benayoun BA, Pollina EA, Singh PP, Mahmoudi S, Harel I, Casey KM, Dulken BW, Kundaje A, Brunet A. Remodeling of epigenome and transcriptome landscapes with aging in mice reveals widespread induction of inflammatory responses. Genome Res. 2019;29:697–709.
pubmed: 30858345
pmcid: 6442391
doi: 10.1101/gr.240093.118
Efremova M, Vento-Tormo M, Teichmann SA, Vento-Tormo R. Cell PhoneDB: inferring cell-cell communication from combined expression of multi-subunit ligand-receptor complexes. Nat Protoc. 2020;15:1484–506.
pubmed: 32103204
doi: 10.1038/s41596-020-0292-x
Tang Z, Li C, Kang B, Gao G, Li C, Zhang Z. GEPIA: a web server for cancer and normal gene expression profiling and interactive analyses. Nucleic Acids Res. 2017;45:W98-w102.
pubmed: 28407145
pmcid: 5570223
doi: 10.1093/nar/gkx247
Liu FY, Zhao ZJ, Li P, Ding X, Guo N, Yang LL, Zong ZH, Sun CF. NF-κB participates in chemokine receptor 7-mediated cell survival in metastatic squamous cell carcinoma of the head and neck. Oncol Rep. 2011;25:383–91.
pubmed: 21165563
McGinnis CS, Murrow LM, Gartner ZJ. DoubletFinder: Doublet Detection in Single-Cell RNA Sequencing Data Using Artificial Nearest Neighbors. Cell Syst. 2019;8:329-337.e324.
pubmed: 30954475
pmcid: 6853612
doi: 10.1016/j.cels.2019.03.003
Aran D, Looney AP, Liu L, Wu E, Fong V, Hsu A, Chak S, Naikawadi RP, Wolters PJ, Abate AR, et al. Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage. Nat Immunol. 2019;20:163–72.
pubmed: 30643263
pmcid: 6340744
doi: 10.1038/s41590-018-0276-y
Li F, Wen H, Bukhari I, Liu B, Guo C, Ren F, Tang Y, Mi Y, Zheng P. Relationship Between CNVs and Immune Cells Infiltration in Gastric Tumor Microenvironment. Front Genet. 2022;13:869967.
pubmed: 35754804
pmcid: 9214698
doi: 10.3389/fgene.2022.869967
Chen K, Wang Y, Hou Y, Wang Q, Long D, Liu X, Tian X, Yang Y. Single cell RNA-seq reveals the CCL5/SDC1 receptor-ligand interaction between T cells and tumor cells in pancreatic cancer. Cancer Lett. 2022;545:215834.
pubmed: 35917973
doi: 10.1016/j.canlet.2022.215834
Chen K, Liu X, Liu W, Wang F, Tian X, Yang Y. Development and validation of prognostic and diagnostic model for pancreatic ductal adenocarcinoma based on scRNA-seq and bulk-seq datasets. Hum Mol Genet. 2022;31:1705–19.
pubmed: 34957503
doi: 10.1093/hmg/ddab343
Patel AP, Tirosh I, Trombetta JJ, Shalek AK, Gillespie SM, Wakimoto H, Cahill DP, Nahed BV, Curry WT, Martuza RL, et al. Single-cell RNA-seq highlights intratumoral heterogeneity in primary glioblastoma. Science. 2014;344:1396–401.
pubmed: 24925914
pmcid: 4123637
doi: 10.1126/science.1254257
Gladka MM, Molenaar B, de Ruiter H, van der Elst S, Tsui H, Versteeg D, Lacraz GPA, Huibers MMH, van Oudenaarden A, van Rooij E. Single-Cell Sequencing of the Healthy and Diseased Heart Reveals Cytoskeleton-Associated Protein 4 as a New Modulator of Fibroblasts Activation. Circulation. 2018;138:166–80.
pubmed: 29386203
doi: 10.1161/CIRCULATIONAHA.117.030742
Pan X, Chen X, Ren Q, Yue L, Niu S, Li Z, Zhu R, Chen X, Jia Z, Zhen R, et al. Single-cell transcriptomics identifies Col1a1 and Col1a2 as hub genes in obesity-induced cardiac fibrosis. Biochem Biophys Res Commun. 2022;618:30–7.
pubmed: 35714568
doi: 10.1016/j.bbrc.2022.06.018
Song Q, Hawkins GA, Wudel L, Chou PC, Forbes E, Pullikuth AK, Liu L, Jin G, Craddock L, Topaloglu U, et al. Dissecting intratumoral myeloid cell plasticity by single cell RNA-seq. Cancer Med. 2019;8:3072–85.
pubmed: 31033233
pmcid: 6558497
doi: 10.1002/cam4.2113
Sreejit G, Abdel-Latif A, Athmanathan B, Annabathula R, Dhyani A, Noothi SK, Quaife-Ryan GA, Al-Sharea A, Pernes G, Dragoljevic D, et al. Neutrophil-Derived S100A8/A9 amplify granulopoiesis after myocardial infarction. Circulation. 2020;141:1080–94.
pubmed: 31941367
pmcid: 7122461
doi: 10.1161/CIRCULATIONAHA.119.043833
Wu H, Dong J, Yu H, Wang K, Dai W, Zhang X, Hu N, Yin L, Tang D, Liu F, Dai Y. Single-Cell RNA and ATAC Sequencing Reveal Hemodialysis-Related Immune Dysregulation of Circulating Immune Cell Subpopulations. Front Immunol. 2022;13:878226.
pubmed: 35720370
pmcid: 9205630
doi: 10.3389/fimmu.2022.878226
Camps M, Nichols A, Arkinstall SJFjopotFoASfEB: Dual specificity phosphatases: a gene family for control of MAP kinase function. FASEB J. 2000;14:6–16.
Barman PK, Shin JE, Lewis SA, Kang S, Wu D, Wang Y, Yang X, Nagarkatti PS, Nagarkatti M, Messaoudi I, et al. Production of MHCII-expressing classical monocytes increases during aging in mice and humans. Aging Cell. 2022;21:e13701.
pubmed: 36040389
pmcid: 9577948
doi: 10.1111/acel.13701
Huse K, Bai B, Hilden VI, Bollum LK, Våtsveen TK, Munthe LA, Smeland EB, Irish JM, Wälchli S, Myklebust JH. Mechanism of CD79A and CD79B support for IgM+ B cell fitness through B cell receptor surface expression. J Immunol. 2022;209:2042–53.
pubmed: 36426942
doi: 10.4049/jimmunol.2200144
Lertkiatmongkol P, Liao D, Mei H, Hu Y, Newman PJ. Endothelial functions of platelet/endothelial cell adhesion molecule-1 (CD31). Curr Opin Hematol. 2016;23:253–9.
pubmed: 27055047
pmcid: 4986701
doi: 10.1097/MOH.0000000000000239
Rong C, Muller MF, Xiang F, Jensen A, Weichert W, Major G, Plinkert PK, Hess J, Affolter A. Adaptive ERK signalling activation in response to therapy and in silico prognostic evaluation of EGFR-MAPK in HNSCC. Br J Cancer. 2020;123:288–97.
pubmed: 32424150
pmcid: 7374086
doi: 10.1038/s41416-020-0892-9
Valentiner U, Knips J, Pries R, Clauditz T, Münscher A, Sauter G, Wollenberg B, Schumacher U. Selectin binding sites are involved in cell adhesive properties of head and neck squamous cell carcinoma. Cancers (Basel). 2019;11:1672.
pubmed: 31661833
doi: 10.3390/cancers11111672
Saint A, Van Obberghen-Schilling E. The role of the tumor matrix environment in progression of head and neck cancer. Curr Opin Oncol. 2021;33:168–74.
pubmed: 33720067
doi: 10.1097/CCO.0000000000000730
Aibar S, González-Blas CB, Moerman T, Huynh-Thu VA, Imrichova H, Hulselmans G, Rambow F, Marine JC, Geurts P, Aerts J, et al. SCENIC: single-cell regulatory network inference and clustering. Nat Methods. 2017;14:1083–6.
pubmed: 28991892
pmcid: 5937676
doi: 10.1038/nmeth.4463
Li Z, Zhang L, Liu FY, Li P, He J, Kirkwood CL, Sohn J, Chan JM, Magner WJ, Kirkwood KL. MKP-1 is required to limit myeloid-cell mediated oral squamous cell carcinoma progression and regional extension. Oral Oncol. 2021;120:105401.
pubmed: 34182221
doi: 10.1016/j.oraloncology.2021.105401
Liu Y, Ji R, Li J, Gu Q, Zhao X, Sun T, Wang J, Li J, Du Q, Sun B. Correlation effect of EGFR and CXCR4 and CCR7 chemokine receptors in predicting breast cancer metastasis and prognosis. J Exp Clin Cancer Res. 2010;29:16.
pubmed: 20181250
pmcid: 2845107
doi: 10.1186/1756-9966-29-16
Cabioglu N, Yazici MS, Arun B, Broglio KR, Hortobagyi GN, Price JE, Sahin A. CCR7 and CXCR4 as novel biomarkers predicting axillary lymph node metastasis in T1 breast cancer. Clin Cancer Res. 2005;11:5686–93.
pubmed: 16115904
doi: 10.1158/1078-0432.CCR-05-0014
Kodama J. Hasengaowa, Seki N, Kusumoto T, Hiramatsu Y: Expression of the CXCR4 and CCR7 chemokine receptors in human endometrial cancer. Eur J Gynaecol Oncol. 2007;28:370–5.
pubmed: 17966215
Kodama J. Hasengaowa, Kusumoto T, Seki N, Matsuo T, Ojima Y, Nakamura K, Hongo A, Hiramatsu Y: Association of CXCR4 and CCR7 chemokine receptor expression and lymph node metastasis in human cervical cancer. Ann Oncol. 2007;18:70–6.
pubmed: 17032700
doi: 10.1093/annonc/mdl342
Wagner PL, Moo TA, Arora N, Liu YF, Zarnegar R, Scognamiglio T, Fahey TJ 3rd. The chemokine receptors CXCR4 and CCR7 are associated with tumor size and pathologic indicators of tumor aggressiveness in papillary thyroid carcinoma. Ann Surg Oncol. 2008;15:2833–41.
pubmed: 18696160
doi: 10.1245/s10434-008-0064-2
Maekawa S, Iwasaki A, Shirakusa T, Kawakami T, Yanagisawa J, Tanaka T, Shibaguchi H, Kinugasa T, Kuroki M, Kuroki M. Association between the expression of chemokine receptors CCR7 and CXCR3, and lymph node metastatic potential in lung adenocarcinoma. Oncol Rep. 2008;19:1461–8.
pubmed: 18497951
Wang Q, Zou H, Wang Y, Shang J, Yang L, Shen J. CCR7-CCL21 axis promotes the cervical lymph node metastasis of tongue squamous cell carcinoma by up-regulating MUC1. J Craniomaxillofac Surg. 2021;49:562–9.
pubmed: 33966967
doi: 10.1016/j.jcms.2021.02.027
Shang ZJ, Liu K, Shao Z. Expression of chemokine receptor CCR7 is associated with cervical lymph node metastasis of oral squamous cell carcinoma. Oral Oncol. 2009;45:480–5.
pubmed: 18752985
doi: 10.1016/j.oraloncology.2008.06.005
Li P, Liu F, Sun L, Zhao Z, Ding X, Shang D, Xu Z, Sun C. Chemokine receptor 7 promotes cell migration and adhesion in metastatic squamous cell carcinoma of the head and neck by activating integrin αvβ3. Int J Mol Med. 2011;27:679–87.
pubmed: 21347514
Liu FY, Safdar J, Li ZN, Fang QG, Zhang X, Xu ZF, Sun CF. CCR7 regulates cell migration and invasion through MAPKs in metastatic squamous cell carcinoma of head and neck. Int J Oncol. 2014;45:2502–10.
pubmed: 25270024
doi: 10.3892/ijo.2014.2674
Liu MD, Wu H, Wang S, Pang P, Jin S, Sun CF, Liu FY. MiR-1275 promotes cell migration, invasion and proliferation in squamous cell carcinoma of head and neck via up-regulating IGF-1R and CCR7. Gene. 2018;646:1–7.
pubmed: 29278769
doi: 10.1016/j.gene.2017.12.049
Wang S, Jin S, Liu MD, Pang P, Wu H, Qi ZZ, Liu FY, Sun CF. Hsa-let-7e-5p inhibits the proliferation and metastasis of head and neck squamous cell carcinoma cells by targeting chemokine receptor 7. J Cancer. 2019;10:1941–8.
pubmed: 31205553
pmcid: 6547991
doi: 10.7150/jca.29536
Chen YP, Yin JH, Li WF, Li HJ, Chen DP, Zhang CJ, Lv JW, Wang YQ, Li XM, Li JY, et al. Single-cell transcriptomics reveals regulators underlying immune cell diversity and immune subtypes associated with prognosis in nasopharyngeal carcinoma. Cell Res. 2020;30:1024–42.
pubmed: 32686767
pmcid: 7784929
doi: 10.1038/s41422-020-0374-x
Lambrechts D, Wauters E, Boeckx B, Aibar S, Nittner D, Burton O, Bassez A, Decaluwé H, Pircher A, Van den Eynde K, et al. Phenotype molding of stromal cells in the lung tumor microenvironment. Nat Med. 2018;24:1277–89.
pubmed: 29988129
doi: 10.1038/s41591-018-0096-5
Peng J, Sun BF, Chen CY, Zhou JY, Chen YS, Chen H, Liu L, Huang D, Jiang J, Cui GS, et al. Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma. Cell Res. 2019;29:725–38.
pubmed: 31273297
pmcid: 6796938
doi: 10.1038/s41422-019-0195-y
Bruna F, Scodeller P. Pro-tumorigenic macrophage infiltration in oral squamous cell carcinoma and possible macrophage-aimed therapeutic interventions. Front Oncol. 2021;11:675664.
pubmed: 34041037
pmcid: 8141624
doi: 10.3389/fonc.2021.675664
Yang L, Zhang Y. Tumor-associated macrophages: from basic research to clinical application. J Hematol Oncol. 2017;10:58.
pubmed: 28241846
pmcid: 5329931
doi: 10.1186/s13045-017-0430-2
Gao L, Zhang W, Zhong WQ, Liu ZJ, Li HM, Yu ZL, Zhao YF. Tumor associated macrophages induce epithelial to mesenchymal transition via the EGFR/ERK1/2 pathway in head and neck squamous cell carcinoma. Oncol Rep. 2018;40:2558–72.
pubmed: 30132555
pmcid: 6151899
Pan Y, Lu F, Fei Q, Yu X, Xiong P, Yu X, Dang Y, Hou Z, Lin W, Lin X, et al. Single-cell RNA sequencing reveals compartmental remodeling of tumor-infiltrating immune cells induced by anti-CD47 targeting in pancreatic cancer. J Hematol Oncol. 2019;12:124.
pubmed: 31771616
pmcid: 6880569
doi: 10.1186/s13045-019-0822-6
Li Z, Liu F, Kirkwood KJOo. The p38/MKP-1 signaling axis in oral cancer: impact of tumor-associated macrophages. Oral Oncol. 2020;103:104591.
pubmed: 32058294
pmcid: 7136140
doi: 10.1016/j.oraloncology.2020.104591
Huang YK, Wang M, Sun Y, Di Costanzo N, Mitchell C, Achuthan A, Hamilton JA, Busuttil RA, Boussioutas A. Macrophage spatial heterogeneity in gastric cancer defined by multiplex immunohistochemistry. Nat Commun. 2019;10:3928.
pubmed: 31477692
pmcid: 6718690
doi: 10.1038/s41467-019-11788-4
Gu B, Kaneko T, Zaw SYM, Sone PP, Murano H, Sueyama Y, Zaw ZCT, Okiji T. Macrophage populations show an M1-to-M2 transition in an experimental model of coronal pulp tissue engineering with mesenchymal stem cells. Int Endod J. 2019;52:504–14.
pubmed: 30387178
doi: 10.1111/iej.13033
Van Raemdonck K, Umar S, Shahrara S. The pathogenic importance of CCL21 and CCR7 in rheumatoid arthritis. Cytokine Growth Factor Rev. 2020;55:86–93.
pubmed: 32499193
pmcid: 10018533
doi: 10.1016/j.cytogfr.2020.05.007
Mueller PA, Zhu L, Tavori H, Huynh K, Giunzioni I, Stafford JM, Linton MF, Fazio S. Deletion of macrophage low-density lipoprotein receptor-related protein 1 (LRP1) accelerates atherosclerosis regression and increases c-c chemokine receptor type 7 (CCR7) expression in plaque macrophages. Circulation. 2018;138:1850–63.
pubmed: 29794082
pmcid: 6343494
doi: 10.1161/CIRCULATIONAHA.117.031702
Chimal-Ramírez GK, Espinoza-Sánchez NA, Chávez-Sánchez L, Arriaga-Pizano L, Fuentes-Pananá EM. Monocyte differentiation towards protumor activity does not correlate with M1 or M2 phenotypes. J Immunol Res. 2016;2016:6031486.
pubmed: 27376091
pmcid: 4916292
doi: 10.1155/2016/6031486
Xuan W, Qu Q, Zheng B, Xiong S, Fan GH. The chemotaxis of M1 and M2 macrophages is regulated by different chemokines. J Leukoc Biol. 2015;97:61–9.
pubmed: 25359998
doi: 10.1189/jlb.1A0314-170R
Hao P, Li H, Lee M, Wang Y, Kim J, Yu G, Lee S, Leem S, Jang K, Kim DJJoh. Disruption of a regulatory loop between DUSP1 and p53 contributes to hepatocellular carcinoma development and progression. J Hepatol. 2015;62:1278–86.
pubmed: 25617504
doi: 10.1016/j.jhep.2014.12.033
Lu N, Malemud CJ. Extracellular signal-regulated kinase: a regulator of cell growth, inflammation, chondrocyte and bone cell receptor-mediated gene expression. Int J Mol Sci. 2019;20:3792.
pubmed: 31382554
pmcid: 6696446
doi: 10.3390/ijms20153792
Duff J, Monia B, Berk BJTJobc. Mitogen-activated protein (MAP) kinase is regulated by the MAP kinase phosphatase (MKP-1) in vascular smooth muscle cells. Effect of actinomycin D and antisense oligonucleotides. J Biol Chem. 1995;270:7161–6.
pubmed: 7706254
doi: 10.1074/jbc.270.13.7161
Chu Y, Solski P, Khosravi-Far R, Der C, Kelly KJTJobc. The mitogen-activated protein kinase phosphatases PAC1, MKP-1, and MKP-2 have unique substrate specificities and reduced activity in vivo toward the ERK2 sevenmaker mutation. J Biol Chem. 1996;271:6497–501.
pubmed: 8626452
doi: 10.1074/jbc.271.11.6497
Slack D, Seternes O, Gabrielsen M, Keyse SJTJobc. Distinct binding determinants for ERK2/p38alpha and JNK map kinases mediate catalytic activation and substrate selectivity of map kinase phosphatase-1. J Biol Chem. 2001;276:16491–500.
pubmed: 11278799
doi: 10.1074/jbc.M010966200
Hutter D, Chen P, Barnes J, Liu YJTBj. Catalytic activation of mitogen-activated protein (MAP) kinase phosphatase-1 by binding to p38 MAP kinase: critical role of the p38 C-terminal domain in its negative regulation. Biochem J. 2000;352 Pt 1:155–63.
pubmed: 11062068
doi: 10.1042/bj3520155
Li Z, Zhang L, Liu F, Li P, He J, Kirkwood C, Sohn J, Chan J, Magner W, Kirkwood KJOo. MKP-1 is required to limit myeloid-cell mediated oral squamous cell carcinoma progression and regional extension. Oral Oncol. 2021;120:105401.
pubmed: 34182221
doi: 10.1016/j.oraloncology.2021.105401