Modulation of Equid Herpesvirus-1 Replication Dynamics

CRISPR/Cas9 EHV-1 antiviral equid alphaherpesvirus-1 gene editing viral replication

Journal

Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722

Informations de publication

Date de publication:
06 Mar 2024
Historique:
received: 05 02 2024
revised: 29 02 2024
accepted: 02 03 2024
medline: 28 3 2024
pubmed: 28 3 2024
entrez: 28 3 2024
Statut: epublish

Résumé

(1) Background: equid alphaherpesvirus-1 (EHV-1) is a highly contagious viral pathogen prevalent in most horse populations worldwide. Genome-editing technologies such as CRISPR/Cas9 have become powerful tools for precise RNA-guided genome modifications; (2) Methods: we designed single guide RNAs (sgRNA) to target three essential (ORF30, ORF31, and ORF7) and one non-essential (ORF74) EHV-1 genes and determine their effect on viral replication dynamics

Identifiants

DOI: 10.3390/v16030409 PMID: 38543774
pubmed: 38543774
pii: v16030409
doi: 10.3390/v16030409
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Auteurs

Rabab T Hassanien (RT)

Louisiana Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Virology Department, Animal Health Research Institute, Agriculture Research Center (ARC), Dokki, Giza 12618, Egypt.
ORCID: 0000-0002-8129-7357

Côme J Thieulent (CJ)

Louisiana Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
ORCID: 0000-0001-7402-5989

Mariano Carossino (M)

Louisiana Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
ORCID: 0000-0003-3864-5915

Ganwu Li (G)

Department of Veterinary Diagnostics and Production Animal Medicine, Iowa State University, Ames, IA 50011, USA.

Udeni B R Balasuriya (UBR)

Louisiana Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
ORCID: 0000-0003-0609-678X

Classifications MeSH