α-Synuclein Pathology in PRKN-Linked Parkinson's Disease: New Insights from a Blood-Based Seed Amplification Assay.


Journal

Annals of neurology
ISSN: 1531-8249
Titre abrégé: Ann Neurol
Pays: United States
ID NLM: 7707449

Informations de publication

Date de publication:
28 Mar 2024
Historique:
revised: 04 03 2024
received: 25 10 2023
accepted: 06 03 2024
medline: 28 3 2024
pubmed: 28 3 2024
entrez: 28 3 2024
Statut: aheadofprint

Résumé

Pathogenic variants in PRKN cause early-onset Parkinson's disease (PD), while the role of alpha-synuclein in PRKN-PD remains uncertain. One study performed a blood-based alpha-synuclein seed amplification assay (SAA) in PRKN-PD, not detecting seed amplification in 17 PRKN-PD patients. By applying a methodologically different SAA focusing on neuron-derived extracellular vesicles, we demonstrated alpha-synuclein seed amplification in 8 of 13 PRKN-PD patients, challenging the view of PRKN-PD as a non-synucleinopathy. Moreover, we performed blinded replication of the neuron-derived extracellular vesicles-dependent SAA in idiopathic PD patients and healthy controls. In conclusion, blood-based neuron-derived extracellular vesicles-dependent SAA represents a promising biomarker to elucidate the underpinnings of (monogenic) PD. ANN NEUROL 2024.

Identifiants

pubmed: 38546204
doi: 10.1002/ana.26917
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : EU Joint Programme - Neurodegenerative Disease Research
Organisme : Deutsche Forschungsgemeinschaft
ID : FOR2488
Organisme : Michael J. Fox Foundation for Parkinson's Research
Organisme : Else Kröner-Fresenius-Stiftung
Organisme : Horizon 2020 Framework Programme
ID : 668738

Informations de copyright

© 2024 The Authors. Annals of Neurology published by Wiley Periodicals LLC on behalf of American Neurological Association.

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Auteurs

Annika Kluge (A)

Department of Neurology, University Hospital Schleswig-Holstein Campus Kiel and Kiel University, Kiel, Germany.

Max Borsche (M)

Institute of Neurogenetics, University of Lübeck, Lübeck, Germany.
Department of Neurology, University Hospital Schleswig-Holstein Campus Lübeck and University of Lübeck, Lübeck, Germany.

Linn Streubel-Gallasch (L)

Institute of Neurogenetics, University of Lübeck, Lübeck, Germany.

Tuğçe Gül (T)

Neurodegeneration Research Laboratory (NDAL), Research Center for Translational Medicine (KUTTAM), University School of Medicine, Istanbul, Turkey.

Susen Schaake (S)

Institute of Neurogenetics, University of Lübeck, Lübeck, Germany.

Alexander Balck (A)

Institute of Neurogenetics, University of Lübeck, Lübeck, Germany.
Department of Neurology, University Hospital Schleswig-Holstein Campus Lübeck and University of Lübeck, Lübeck, Germany.

Jannik Prasuhn (J)

Institute of Neurogenetics, University of Lübeck, Lübeck, Germany.
Department of Neurology, University Hospital Schleswig-Holstein Campus Lübeck and University of Lübeck, Lübeck, Germany.

Philip Campbell (P)

Department of Clinical and Movement Neurosciences, UCL Queen Square Institute of Neurology, University College London, London, UK.
UCL Movement Disorders Center, University College London, London, UK.

Huw R Morris (HR)

Department of Clinical and Movement Neurosciences, UCL Queen Square Institute of Neurology, University College London, London, UK.
UCL Movement Disorders Center, University College London, London, UK.

Anthony H Schapira (AH)

Department of Clinical and Movement Neurosciences, UCL Queen Square Institute of Neurology, University College London, London, UK.
UCL Movement Disorders Center, University College London, London, UK.

Katja Lohmann (K)

Institute of Neurogenetics, University of Lübeck, Lübeck, Germany.

Norbert Brüggemann (N)

Institute of Neurogenetics, University of Lübeck, Lübeck, Germany.
Department of Neurology, University Hospital Schleswig-Holstein Campus Lübeck and University of Lübeck, Lübeck, Germany.

Aleksandar Rakovic (A)

Institute of Neurogenetics, University of Lübeck, Lübeck, Germany.

Philip Seibler (P)

Institute of Neurogenetics, University of Lübeck, Lübeck, Germany.

A Nazlı Başak (AN)

Neurodegeneration Research Laboratory (NDAL), Research Center for Translational Medicine (KUTTAM), University School of Medicine, Istanbul, Turkey.

Daniela Berg (D)

Department of Neurology, University Hospital Schleswig-Holstein Campus Kiel and Kiel University, Kiel, Germany.

Christine Klein (C)

Institute of Neurogenetics, University of Lübeck, Lübeck, Germany.

Classifications MeSH