Macrophage migration inhibitory factor blockade reprograms macrophages and disrupts prosurvival signaling in acute myeloid leukemia.


Journal

Cell death discovery
ISSN: 2058-7716
Titre abrégé: Cell Death Discov
Pays: United States
ID NLM: 101665035

Informations de publication

Date de publication:
28 Mar 2024
Historique:
received: 29 09 2023
accepted: 19 03 2024
revised: 14 03 2024
medline: 29 3 2024
pubmed: 29 3 2024
entrez: 29 3 2024
Statut: epublish

Résumé

The malignant microenvironment plays a major role in the development of resistance to therapies and the occurrence of relapses in acute myeloid leukemia (AML). We previously showed that interactions of AML blasts with bone marrow macrophages (MΦ) shift their polarization towards a protumoral (M2-like) phenotype, promoting drug resistance; we demonstrated that inhibiting the colony-stimulating factor-1 receptor (CSF1R) repolarizes MΦ towards an antitumoral (M1-like) phenotype and that other factors may be involved. We investigated here macrophage migration inhibitory factor (MIF) as a target in AML blast survival and protumoral interactions with MΦ. We show that pharmacologically inhibiting MIF secreted by AML blasts results in their apoptosis. However, this effect is abrogated when blasts are co-cultured in close contact with M2-like MΦ. We next demonstrate that pharmacological inhibition of MIF secreted by MΦ, in the presence of granulocyte macrophage-colony stimulating factor (GM-CSF), efficiently reprograms MΦ to an M1-like phenotype that triggers apoptosis of interacting blasts. Furthermore, contact with reprogrammed MΦ relieves blast resistance to venetoclax and midostaurin acquired in contact with CD163

Identifiants

pubmed: 38548753
doi: 10.1038/s41420-024-01924-5
pii: 10.1038/s41420-024-01924-5
doi:

Types de publication

Journal Article

Langues

eng

Pagination

157

Informations de copyright

© 2024. The Author(s).

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Auteurs

Caroline Spertini (C)

Service and Central Laboratory of Hematology, Lausanne University Hospital (CHUV), 1011, Lausanne, Switzerland.

Alexandre P Bénéchet (AP)

In Vivo Imaging Facility (IVIF), Department of Research and Training, Lausanne University Hospital and University of Lausanne, Lausanne, 1011, Switzerland.

Flora Birch (F)

Department of oncology UNIL-CHUV, Lausanne University Hospital (CHUV), University of Lausanne (UNIL), 1011, Lausanne, Switzerland.
Ludwig Institute for Cancer Research Lausanne, 1015, Lausanne, Switzerland.

Axel Bellotti (A)

Service and Central Laboratory of Hematology, Lausanne University Hospital (CHUV), 1011, Lausanne, Switzerland.

Mónica Román-Trufero (M)

Service and Central Laboratory of Hematology, Lausanne University Hospital (CHUV), 1011, Lausanne, Switzerland.

Caroline Arber (C)

Service and Central Laboratory of Hematology, Lausanne University Hospital (CHUV), 1011, Lausanne, Switzerland.
Department of oncology UNIL-CHUV, Lausanne University Hospital (CHUV), University of Lausanne (UNIL), 1011, Lausanne, Switzerland.
Ludwig Institute for Cancer Research Lausanne, 1015, Lausanne, Switzerland.
Faculty of Biology and Medicine, University of Lausanne, 1011, Lausanne, Switzerland.
Service of Immuno-oncology, Lausanne University Hospital (CHUV), 1011, Lausanne, Switzerland.

Holger W Auner (HW)

Service and Central Laboratory of Hematology, Lausanne University Hospital (CHUV), 1011, Lausanne, Switzerland.
Faculty of Biology and Medicine, University of Lausanne, 1011, Lausanne, Switzerland.

Robert A Mitchell (RA)

Department of Surgery, Division of Immunotherapy, University of Louisville, Louisville, KY, 40202, USA.

Olivier Spertini (O)

Faculty of Biology and Medicine, University of Lausanne, 1011, Lausanne, Switzerland.

Tatiana Smirnova (T)

Service and Central Laboratory of Hematology, Lausanne University Hospital (CHUV), 1011, Lausanne, Switzerland. tatiana.smirnova@chuv.ch.

Classifications MeSH