Real-time analysis of osteoclast resorption and fusion dynamics in response to bone resorption inhibitors.

Active-site probe Bone resorption Cathepsin K Cell fusion Human osteoclast Live-imaging

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
28 Mar 2024
Historique:
received: 18 09 2023
accepted: 19 03 2024
medline: 29 3 2024
pubmed: 29 3 2024
entrez: 29 3 2024
Statut: epublish

Résumé

Cathepsin K (CatK), an essential collagenase in osteoclasts (OCs), is a potential therapeutic target for the treatment of osteoporosis. Using live-cell imaging, we monitored the bone resorptive behaviour of OCs during dose-dependent inhibition of CatK by an ectosteric (Tanshinone IIA sulfonate) and an active site inhibitor (odanacatib). CatK inhibition caused drastic reductions in the overall resorption speed of OCs. At IC

Identifiants

DOI: 10.1038/s41598-024-57526-9 PMID: 38548807
pubmed: 38548807
doi: 10.1038/s41598-024-57526-9
pii: 10.1038/s41598-024-57526-9
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

7358

Subventions

Organisme : CIHR
ID : CPG-158275
Pays : Canada

Informations de copyright

© 2024. The Author(s).

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Auteurs

Preety Panwar (P)

Department of Biochemistry and Molecular Biology, Faculty of Medicine, The University of British Columbia, Vancouver, BC, Canada.
Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, V6T 1Z3, Canada.
Department of Pharmaceutical Sciences, Elizabeth City State University, Elizabeth City, NC, USA.

Jacob Bastholm Olesen (JB)

Clinical Cell Biology, Pathology Research Unit, Department of Clinical Research, University of Southern Denmark, Campusvej 55, 5230, Odense M, Denmark.
Department of Pathology, Odense University Hospital, Odense, Denmark.

Galia Blum (G)

Faculty of Medicine, Campus Ein Karem, The School of Pharmacy, Institute of Drug Research, The Hebrew University of Jerusalem, Room 407, 9112001, Jerusalem, Israel.

Jean-Marie Delaisse (JM)

Clinical Cell Biology, Pathology Research Unit, Department of Clinical Research, University of Southern Denmark, Campusvej 55, 5230, Odense M, Denmark.

Kent Søe (K)

Clinical Cell Biology, Pathology Research Unit, Department of Clinical Research, University of Southern Denmark, Campusvej 55, 5230, Odense M, Denmark. kent.soee@rsyd.dk.
Department of Pathology, Odense University Hospital, Odense, Denmark. kent.soee@rsyd.dk.
Department of Molecular Medicine, University of Southern Denmark, Odense, Denmark. kent.soee@rsyd.dk.

Dieter Brömme (D)

Department of Biochemistry and Molecular Biology, Faculty of Medicine, The University of British Columbia, Vancouver, BC, Canada. dbromme@dentistry.ubc.ca.
Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, V6T 1Z3, Canada. dbromme@dentistry.ubc.ca.

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