The immunoglobulin heavy chain super enhancer controls class switch recombination in developing B cells.
IgH locus
Class switch recombination
Early B cells
Super-enhancer
Switch transcription
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
28 Mar 2024
28 Mar 2024
Historique:
received:
08
12
2023
accepted:
19
03
2024
medline:
29
3
2024
pubmed:
29
3
2024
entrez:
29
3
2024
Statut:
epublish
Résumé
Class switch recombination (CSR) plays an important role in adaptive immune response by enabling mature B cells to replace the initial IgM by another antibody class (IgG, IgE or IgA). CSR is preceded by transcription of the IgH constant genes and is controlled by the super-enhancer 3' regulatory region (3'RR) in an activation-specific manner. The 3'RR is composed of four enhancers (hs3a, hs1-2, hs3b and hs4). In mature B cells, 3'RR activity correlates with transcription of its enhancers. CSR can also occur in primary developing B cells though at low frequency, but in contrast to mature B cells, the transcriptional elements that regulate the process in developing B cells are ill-known. In particular, the role of the 3'RR in the control of constant genes' transcription and CSR has not been addressed. Here, by using a mouse line devoid of the 3'RR and a culture system that highly enriches in pro-B cells, we show that the 3'RR activity is indeed required for switch transcription and CSR, though its effect varies in an isotype-specific manner and correlates with transcription of hs4 enhancer only.
Identifiants
pubmed: 38548819
doi: 10.1038/s41598-024-57576-z
pii: 10.1038/s41598-024-57576-z
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7370Subventions
Organisme : Agence Nationale de la Recherche
ID : AID-G4-CSR, AAP CE15
Organisme : Agence Nationale de la Recherche
ID : AID-G4-CSR, AAP CE15
Organisme : Fondation ARC pour la Recherche sur le Cancer
ID : PJA 20191209515
Informations de copyright
© 2024. The Author(s).
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