Intracellular and Extracellular Peptidomes of the Model Plant, Physcomitrium patens.

Mass spectrometry Peptidomics Physcomitrium patens Size exclusion chromatography Solid phase extraction

Journal

Methods in molecular biology (Clifton, N.J.)

ISSN: 1940-6029

Titre abrégé: Methods Mol Biol

Pays: United States

ID NLM: 9214969

Informations de publication

Date de publication:
2024

Historique:

medline: 29 3 2024

pubmed: 29 3 2024

entrez: 29 3 2024

Statut: ppublish

Résumé

Here, we report our approach to peptidomic analysis of the plant model Physcomitrium patens. Intracellular and extracellular peptides were extracted under conditions preventing proteolytic digestion by endogenous proteases. The extracts were fractionated on size exclusion columns to isolate intracellular peptides and on reversed-phase cartridges to isolate extracellular peptides, with the isolated peptides subjected to LC-MS/MS analysis. Mass spectrometry data were analyzed for the presence of peptides derived from the known proteins or microproteins encoded by small open reading frames (<100 aa, smORFs) predicted in the moss genome. Experimental details are provided for each step.

Identifiants

DOI: 10.1007/978-1-0716-3646-6_20 PMID: 38549025

pubmed: 38549025

doi: 10.1007/978-1-0716-3646-6_20

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

375-385

Informations de copyright

Références

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